Aerobic type high-efficiency calcium mineralized bacillus and application thereof in concrete repair

A Bacillus, high-efficiency technology, applied to the high-efficiency calcium-mineralizing alkalophilic Bacillus and its application in the repair of building materials, can solve problems such as weakening of durability, damage to concrete integrity, and occurrence of micro-cracks

Active Publication Date: 2018-06-01
SHENZHEN UNIV
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Problems solved by technology

However, with the increase of the service life and various environmental conditions, such as the influence of physical or chemical erosion such as atmosphere and water, micro-cracks will begin to appear on the surface and inside of the concrete
If effective treatment measures are not taken in time, once the cracks expand, the internal steel structure will be corroded, resulting in damage to the integrity of the concrete, and the durability will be weakened (environmental erosion of reinforced concrete)

Method used

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  • Aerobic type high-efficiency calcium mineralized bacillus and application thereof in concrete repair
  • Aerobic type high-efficiency calcium mineralized bacillus and application thereof in concrete repair
  • Aerobic type high-efficiency calcium mineralized bacillus and application thereof in concrete repair

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Embodiment Construction

[0013] The acquisition process and strain characteristics of the strains involved in the present invention will be described in detail below in conjunction with the accompanying drawings.

[0014] 1. Isolation, purification and preservation of Bacillus B6.

[0015] The calcium ion mineralizing bacteria are obtained through enrichment, screening, purification and isolation, and the specific steps are as follows:

[0016] Enrichment culture: Add 1 g each of saline-alkali lake sediment and mangrove sediment soil samples to nitrogen-free LN-seawater-sodium carbonate culture solution, and culture on a shaker at 30°C and 150 rpm for 7 days.

[0017] Separation culture: draw 200 μL of enriched culture solution and add it to the surface of nitrogen-free LN-seawater-sodium carbonate solid medium plate, spread evenly with a sterile coating rod, and then use the coating rod to continue coating 3 nitrogen-free LN- The seawater-sodium carbonate solid medium plate was cultured upside down ...

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Abstract

The invention discloses an aerobic type high-efficiency calcium mineralized bacillus and application thereof in concrete repair. The Latin name of the strain of the high-efficiency calcium mineralizedbacillus is Bacillus sp.B6, and is identified as Bacillus, and is preserved in China Centre for Type Culture Collection; the preservation number is CGMCC NO.13360; the bacterium has high-efficiency calcium ion deposition activity; under the condition that calcium peroxide provides oxygen and calcium ions in a water environment, the yield of calcium carbonate formed on the wall of a glass test pipe is up to 54 mmol / L through 30 days of culture when initial cell concentration is 1*10<9> cells / ml; and the bacterium has good reproductive capacity and capacity of inducing calcium carbonate deposition in a strong alkaline environment, and can be used for repairing a concrete crack.

Description

[technical field] [0001] The invention belongs to the field of biotechnology, and in particular relates to a high-efficiency calcium-mineralizing alkalophilic bacillus and its application in repairing building materials. [Background technique] [0002] As the most widely used civil engineering material in the world, concrete is widely used in modern construction projects because of its firm structure, strong durability, abundant raw materials, and low price. However, with the increase of the service life and various environmental conditions, such as the influence of physical or chemical erosion such as atmosphere and water, micro-cracks will begin to appear on the surface and inside of the concrete. If effective treatment measures are not taken in time, once the cracks expand, the internal steel structure will be corroded, resulting in damage to the integrity of the concrete, and the durability will be weakened (environmental erosion of reinforced concrete). However, some s...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20E04G23/02C12R1/07
CPCC12N1/205C12R2001/07E04G23/02
Inventor 张金龙邓旭邢锋韩宁旭刘冰麦碧霞张婉仪董晨辉吴婉涵宋瑜君
Owner SHENZHEN UNIV
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