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Culture method of Lactoacillus sanfanciscensis Ls-1001 bacterial strain high in exopolysaccharides yield

A technology of Lactobacillus San Francisco and exopolysaccharides, which is applied in the field of high-yield exopolysaccharide Lactobacillus San Francisco Ls-1001 strain cultivation, can solve the problems that the strain characteristics and cultivation conditions have not yet been clearly understood, and achieve the effect of shortening the cultivation time and significantly increasing production Effect

Active Publication Date: 2018-06-01
SHANXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the industrial production of Lactobacillus San Francisco is not yet mature. The main reason is that the characteristics and culture conditions of the strain have not yet been clearly understood.

Method used

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  • Culture method of Lactoacillus sanfanciscensis Ls-1001 bacterial strain high in exopolysaccharides yield
  • Culture method of Lactoacillus sanfanciscensis Ls-1001 bacterial strain high in exopolysaccharides yield
  • Culture method of Lactoacillus sanfanciscensis Ls-1001 bacterial strain high in exopolysaccharides yield

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Experimental program
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Embodiment 1

[0027] The present embodiment has selected five kinds of culture medium to carry out experiment respectively:

[0028] ①SDB medium: 6g / L peptone, 3g / L yeast powder, 20g / L maltose, 0.03% Tween 80 (3mL10% Tween / L), 1.5% fresh yeast extract, pH5.6;

[0029] Further, the preparation method of the fresh yeast extract is as follows: 20% commercially available high-activity dry yeast is dissolved in distilled water, sterilized under high pressure at 121°C for 30 minutes, cooled and placed overnight at 4°C, centrifuged and packaged, and stored at -80°C.

[0030] ②MRS medium: 10g / L peptone, 4g / L yeast powder, 5g / L beef extract powder, 20g / L glucose, 5g / L sodium acetate, 2g / L dipotassium hydrogen phosphate, 2g / L ammonium citrate, 0.2g / L magnesium sulfate, 0.05g / L manganese sulfate, 1mL / L Tween 80, pH5.4.

[0031] ③mMRS medium: 10g / L peptone, 5g / L yeast powder, 10g / L beef extract powder, 20g / L maltose, 5g / L sodium acetate, 2g / L dipotassium hydrogen phosphate, 2g / L ammonium citrate, 0.2...

Embodiment 2

[0036] The screening of embodiment 2 growth factors

[0037] On the basis of the optimal medium mMRS, two kinds of growth factors compounded with B vitamins and fresh yeast extract were studied, and the addition amount was 1.0g / L and 15mL / L respectively. The inoculum amount of the seed solution in the fermentation medium was 1%, the culture temperature was 30°C, and the initial pH was 5.4, and it was placed in an anaerobic incubator for static culture for 36 hours, and the number of colonies in the culture medium was measured.

[0038] figure 2 Show the impact of growth factors on the production of Lactobacillus San Francisco Ls-1001 bacterial classification, by figure 2 It can be seen that the complex B vitamins have a more significant effect on the growth of the strain than the fresh yeast extract, and the number of viable bacteria is as high as 2.77×10 8 CFU / mL. Therefore, complex B vitamins were selected as the growth-promoting factor of Lactobacillus San Francisco L...

Embodiment 3

[0039] The output of bacterial classification under embodiment 3 different times

[0040] image 3 Show the variation of Lactobacillus San Francisco Ls-1001 bacterial classification yield under different time, by image 3 It can be seen that the number of colonies tends to be stable when the culture time reaches 36 h. It can be concluded that the optimal culture time of Lactobacillus San Francisco Ls-1001 is 36 hours, which is significantly shortened from the 48 hours previously reported by Christiane B et al.

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Abstract

The invention discloses a culture method of Lactoacillus sanfanciscensis Ls-1001 bacterial strain high in exopolysaccharides yield. The culture method comprises following steps: frozen Lactoacillus sanfanciscensis slant strains are diluted with sterilized normal saline until the absorbance value at 600nm A=0.5; 100ml of a liquid culture medium containing growth-promoting factors is inoculated withan obtained diluted solution at a volume fraction of 2% for 34 to 36h of anaerobic cultivation, wherein the pH value of the culture medium is controlled to be 5.0 to 5.4, the culture temperature is controlled to be 30 to 32 DEG C. The Lactoacillus sanfanciscensis Ls-1001 bacterial strain yield, and the exopolysaccharides yield are increased by 4.6 times and 1.4 times respectively, the culture time is shortened by 10 to 12h, obvious yield increasing effect is achieved, and foundation is provided for later industrialized production.

Description

technical field [0001] The invention relates to a method for cultivating a high-yield extracellular polysaccharide Lactobacillus San Francisco Ls-1001 strain, belonging to food microorganisms [0002] field. Background technique [0003] Sourdough, generally known as "old noodles", "fermentation", "face fat" etc. in China, has a long history of use. In the 1980s, instant active dry yeast was introduced into my country. Because of its convenient use, the traditional pasta starter was gradually replaced. However, until now, it is still used in many areas, especially rural areas, to prepare steamed bread and other staple foods. Because of the use of traditional pasta The quality of the steamed bread prepared by the leavening agent is better. The reason is that the complex microbial system makes the fermented steamed bread have the best flavor and aroma, which has a good impact on the sensory quality. The application of Lactobacillus San Francisco has good effects on the flavo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/38C12R1/225
CPCC12N1/20C12N1/38
Inventor 张国华张纬珍刘俊峰孙瑜嵘何国庆
Owner SHANXI UNIV