Soybean protein isolate powder as well as preparation method and use thereof
A technology of protein powder and soybean, which is applied in the field of soybean protein isolate powder and its preparation, which can solve the problems of turbidity and opacity of the solution, decrease of hydrophobic force, and influence on product properties, and achieve the effects of improving transparency, enhancing solubility, and improving taste
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[0064] Example 1
[0065] The defatted soybean meal was mixed with water in a mass ratio of 1:8, stirred at a medium speed for 1 hour at room temperature, adjusted to pH 8 with 2 mol / L NaOH, and sheared at 13,000 rpm for 3 minutes. Acidase and 0.05‰ of laccase were added, reacted at 35° C. for 30 minutes, and then centrifuged at 3000 rpm for 10 minutes to obtain alkali-extracted soybean emulsion and soybean residue. The bean dregs are subjected to shearing treatment, and process water is added according to 1:5 of the soybean meal to perform water extraction, and then centrifuged at 3000 rpm for 10 minutes to obtain water-extracted soybean emulsion and water-extracted soybean dregs. The soymilk obtained by the alkali extraction soybean emulsion and the water extraction soybean emulsion is mixed to obtain the mixed soybean milk. The mixed soybean emulsion was taken and adjusted to pH 4.5 with 2 mol / L HCL, and centrifuged at 3000 rpm for 10 min to obtain soybean protein precipit...
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[0067] Example 2
[0068] The defatted soybean meal was mixed with water in a mass ratio of 1:9, stirred at a medium speed for 1 hour at room temperature, adjusted to pH 9 with 2 mol / L NaOH, and sheared at 13,000 rpm for 3 minutes. Acidase and 0.1‰ of laccase were added, reacted at 35°C for 30 minutes, and then centrifuged at 3000 rpm for 10 minutes to obtain alkali-extracted soybean emulsion and soybean dregs. The bean dregs are subjected to shearing treatment, and process water is added according to 1:5 of the soybean meal to perform water extraction, and then centrifuged at 3000 rpm for 10 minutes to obtain water-extracted soybean emulsion and water-extracted soybean dregs. The soymilk obtained by the alkali extraction soybean emulsion and the water extraction soybean emulsion is mixed to obtain the mixed soybean milk. The mixed soybean emulsion was taken and adjusted to pH 4.5 with 2 mol / L HCL, and centrifuged at 3000 rpm for 10 min to obtain soybean protein precipitation...
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[0070] Example 3
[0071] The defatted soybean meal was mixed with water at a mass ratio of 1:10, stirred at a medium speed for 1 hour at room temperature, adjusted to pH 9.5 with 2 mol / L NaOH, and sheared at 15,000 rpm for 2 minutes. Acidase and 0.5‰ of laccase were added, reacted at 35°C for 30 minutes, and then centrifuged at 3000 rpm for 10 minutes to obtain alkali-extracted soybean emulsion and soybean dregs. The soybean dregs are subjected to shearing treatment, and process water is added according to 1:4 of the soybean meal to perform water extraction, and then centrifuged at 3000 rpm for 10 minutes to obtain water-extracted soybean emulsion and water-extracted soybean dregs. The soymilk obtained by the alkali extraction soybean emulsion and the water extraction soybean emulsion is mixed to obtain the mixed soybean milk. The mixed soybean emulsion was taken and adjusted to pH 4.5 with 2 mol / L HCL, and centrifuged at 3000 rpm for 10 min to obtain soybean protein precipi...
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