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Reagent for detecting urine microalbumin and application of reagent

A microalbumin and urine technology, applied in the field of medical testing, can solve the problems of high false positives, inability to give content, insufficient precision and accuracy of test results, etc., to achieve high accuracy and precision, improve precision and the effects of accuracy, compensating for lack of accuracy and precision

Active Publication Date: 2018-06-05
吉林省汇酉生物技术股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the test paper for detecting microalbumin in the market is relatively well-known in South Korea, but it is a semi-quantitative detection method, which can only give an approximate content range, but not an accurate content, and the false positives are high.
[0005] Chinese patent CN103901034A discloses a reagent strip for detecting urinary microalbumin, which can perform semi-quantitative detection and quantitative detection, but the precision and accuracy of the detection results are all insufficient due to inappropriate reagent selection

Method used

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  • Reagent for detecting urine microalbumin and application of reagent
  • Reagent for detecting urine microalbumin and application of reagent
  • Reagent for detecting urine microalbumin and application of reagent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Example 1: Reagents and test strips of the present invention

[0040] 1. Reagent composition

[0041] Liquid A is: 133 g of sodium oxalate, 21 g of sodium molybdate (2.1%), the volume of purified water is adjusted to 1000 mL, and the pH value is 3.2.

[0042] Liquid B is: 10 g citric acid (1.0%), 30 mL polypropylene glycol (3.0%), 400 mg phenolphthalein dye (0.04%), and make up to 1000 mL with tetrahydrofuran and toluene (volume ratio 1:1).

[0043] 2. Preparation of test strips

[0044] Dip the filter paper in solution A, absorb the excess solution, and dry it in a drying oven at a drying temperature of 100 °C and a drying time of 30 minutes;

[0045]Dip the dried filter paper soaked in solution A in solution B, absorb excess solution, and dry in a drying oven at a drying temperature of 60°C and a drying time of 30 minutes.

[0046] The dried filter paper is cut into strips, pasted on the PVC sheet, and then cut into suitable strips.

Embodiment 2

[0047] Example 2: Reagents and test strips of the present invention

[0048] 1. Reagent composition

[0049] Liquid A is: 133g sodium oxalate, 12g sucrose (1.2%), purified water to 1000mL, pH value 3.2.

[0050] Liquid B is: 12g citric acid (1.2%), 40mL polypropylene glycol (4.0%), 600mg phenolphthalein dye (0.06%), make up to 1000mL with tetrahydrofuran and toluene (volume ratio 1:1.5).

[0051] 2. Preparation of test strips

[0052] Dip the filter paper in solution A, absorb the excess solution, and dry it in a drying oven at a drying temperature of 100 °C and a drying time of 30 minutes;

[0053] Dip the dried filter paper soaked in solution A in solution B, absorb excess solution, and dry in a drying oven at a drying temperature of 60°C and a drying time of 30 minutes.

[0054] The dried filter paper is cut into strips, pasted on the PVC sheet, and then cut into suitable strips.

Embodiment 3

[0055] Example 3: Reagents and test strips of the present invention

[0056] 1. Reagent composition

[0057] Liquid A is: 133g sodium oxalate, 23g sodium molybdate (2.3%), 13g sucrose (1.3%), purified water to 1000mL, pH 3.2.

[0058] Liquid B is: 13g citric acid (1.3%), 50mL polypropylene glycol (5.0%), 800mg phenolphthalein dye (0.08%), and make up to 1000mL with tetrahydrofuran and toluene (1:1.5 by volume).

[0059] 2. Preparation of test strips

[0060] Dip the filter paper in solution A, absorb the excess solution, and dry it in a drying oven at a drying temperature of 100 °C and a drying time of 30 minutes;

[0061] Dip the dried filter paper soaked in solution A in solution B, absorb excess solution, and dry in a drying oven at a drying temperature of 60°C and a drying time of 30 minutes.

[0062] The dried filter paper is cut into strips, pasted on the PVC sheet, and then cut into suitable strips.

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Abstract

The invention relates to the technical field of medical detection and discloses a reagent for detecting urine microalbumin and application of the reagent. The reagent comprises a solution A and a solution B, wherein the solution A is a sodium oxalate buffer solution containing sodium oxalate and at least one or two out of sodium molybdate and saccharose; and the solution B adopts furan and methylbenzene as solvents and contains citric acid, polypropylene glycol and a phenothalin dye. By adjusting and optimizing the components in the reagent, the influences caused by each interference factor ina urine sample to the detection are reduced, relatively high accuracy and precision are achieved, the linear relation is good in a large range, and the reliability is high, so that the defects that existing similar products are poor in accuracy and precision are remedied.

Description

technical field [0001] The invention relates to the technical field of medical detection, in particular to a reagent for detecting urine microalbumin and its application. Background technique [0002] Urinary microalbumin is a type of microalbumin that occurs in urine and is normally present in very small amounts in urine. When kidney disease occurs, it changes the ability of the kidney to filter albumin, causing albumin to leak into the urine. Therefore, urinary microalbumin is the gold standard for early kidney damage. [0003] At present, the detection methods of urine microalbumin include: wet chemical quantitative detection, dry chemical qualitative detection and dry chemical semi-quantitative detection, etc. Among them, wet chemical quantitative detection takes a long time according to the detection time of different automatic biochemical analyzers. ; Dry chemical semi-quantitative detection is more suitable for rough screening. [0004] At present, the most well-kno...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78
CPCG01N21/78
Inventor 朱小艾韩丹丹沈宣辛董理
Owner 吉林省汇酉生物技术股份有限公司
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