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Microfluidic chip surface hydrophilic modification method

A technology of microfluidic chip and modification method, which is applied in the field of analysis and detection, and can solve the problems of easy damage of the chip, non-specific adsorption, and short retention time of hydrophilicity, so as to achieve reduced adsorption, strong hydrophilicity, and wide application range Effect

Inactive Publication Date: 2018-06-08
SHENZHEN MERRIME NANOPORE TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] For this reason, the technical problem to be solved by the present invention is that the surface modification method of the existing microfluidic chip has a short hydrophilic retention time, the chip is easily damaged during the modification process, and the microfluidic chip is easy to non-specifically adsorb the target detection molecule. Therefore, a method for surface hydrophilic modification of microfluidic chips with high modification efficiency, long-lasting hydrophilicity after modification, no damage to the chip and no adsorption to target detection molecules is proposed.

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  • Microfluidic chip surface hydrophilic modification method

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Embodiment 1

[0029] This embodiment provides a method for hydrophilic modification of the surface of a Su8 microfluidic chip with Ag / AgCl thin film electrodes, which includes the following steps:

[0030] S1, prepare dopamine solution, weigh 50mg dopamine powder, dissolve it in 50mL Tris·HCl buffer solution, the concentration of said dopamine is 1mg / mL, the pH=8.5 of said Tris·HCl buffer solution, its concentration is 50mM, obtain Dopamine solution.

[0031] S2. Dopamine-modified microfluidic chip, preparing a Su8 microfluidic chip with Ag / AgCl thin film electrodes as a chip with a size of 1cm*1cm, and placing it in the dopamine solution prepared in step S1, and a container for containing the dopamine solution Open, at 25°C, isolate the magnetic stirring reaction at a stirring speed of 400r / min for 4h. The isolated magnetic stirring refers to using a porous sieve-like PTFE sheet to isolate the magnetons from the microfluidic chip and perform magnetic stirring. After the reaction, the micr...

Embodiment 2

[0034] This embodiment provides a method for hydrophilic modification of the surface of a Su8 microfluidic chip with Ag / AgCl thin film electrodes, which includes the following steps:

[0035] S1, prepare dopamine solution, weigh 50mg dopamine powder, dissolve it in 50mL Tris·HCl buffer solution, the concentration of said dopamine is 1mg / mL, the pH=8.5 of said Tris·HCl buffer solution, its concentration is 50mM, obtain Dopamine solution.

[0036] S2. Dopamine-modified microfluidic chip, preparing a Su8 microfluidic chip with Ag / AgCl thin film electrodes as a chip with a size of 1cm*1cm, and placing it in the dopamine solution prepared in step S1, and a container for containing the dopamine solution Open, at 25°C, isolate the magnetic stirring reaction at a stirring speed of 100r / min for 24h. The isolated magnetic stirring refers to using a porous sieve-shaped PTFE sheet to isolate the magneton from the microfluidic chip and perform magnetic stirring. After the reaction, the micr...

Embodiment 3

[0040] This embodiment provides a method for hydrophilic modification of the surface of a Su8 microfluidic chip with Ag / AgCl thin film electrodes, which includes the following steps:

[0041] S1. Prepare a dopamine solution, weigh 20 mg of dopamine powder, and dissolve it in 40 mL of Tris HCl buffer solution, the dopamine concentration is 0.5 mg / mL, the pH of the Tris HCl buffer solution is 8, and its concentration is 50 mM, to obtain a dopamine solution.

[0042] S2. Dopamine-modified microfluidic chip, preparing a Su8 microfluidic chip with Ag / AgCl thin film electrodes as a chip with a size of 1cm*1cm, and placing it in the dopamine solution prepared in step S1, and a container for containing the dopamine solution Open, at 25°C, isolate the magnetic stirring reaction at a stirring speed of 500r / min for 1h. The isolated magnetic stirring refers to using a porous sieve-shaped PTFE sheet to isolate the magneton from the microfluidic chip and perform magnetic stirring. After th...

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Abstract

The invention discloses a microfluidic chip surface hydrophilic modification method. Reaction conditions are mild, the reaction can be carried out quickly in an aqueous solution under the condition ofnormal temperature, and the reaction conditions cannot cause damage to microstructures of electrodes, detectors and the like inside a chip. The adhesive power of polydopamine attached on the surfaceof the chip is strong, and hydrophilic amino groups on the polydopamine molecules can maintain the hydrophilicity of the chip surface and the channel interior for a long time. In addition, a benzoquinone structure formed after the oxidation in polydopamine quite easily undergoes a Michael addition reaction and a Schiff base reaction with molecules containing sulfydryl (-SH) and amino groups (-NH2); through the reaction, the surface of the chip can also be further modified with PEG and other functional molecules. The hydrophilicity of the chip is significantly improved and can be kept for a long time, and the adsorption of charged molecules can be reduced.

Description

technical field [0001] The invention belongs to the technical field of analysis and detection, and relates to a microfluidic chip surface treatment process, in particular to a microfluidic chip surface polymer hydrophilic modification method. Background technique [0002] Since the concept of micro-analysis system was first proposed in 1990, microfluidic technology was rated as one of the 15 most important inventions affecting the future of mankind in 2003. Microfluidic technology has developed rapidly, among which microfluidic chips As an important development frontier of current analytical science, technology plays a huge role in biology, chemistry, medicine and other fields. Microfluidic chip technology, also known as "lab on a chip", is a highly integrated biochemical automated analysis technology platform, which has huge applications in the fields of biophysical analysis, chemical detection, and clinical diagnosis of diseases. prospect. [0003] Microfluidic chips gen...

Claims

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Application Information

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IPC IPC(8): B01L3/00
Inventor 文豪
Owner SHENZHEN MERRIME NANOPORE TECH CO LTD
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