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A kind of composite microbial agent and its preparation method and application

A technology of compound bacterial agent and pediococcus, which is applied in silage, compound bacterial agent and its preparation, and the application field of corn cob silage, can solve the problems of low fermentation quality and dry matter loss, and achieve large lactic acid production and survival The effect of strong ability and high starch content

Active Publication Date: 2021-08-17
SHANXI AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In order to solve the problems of severe dry matter loss and low fermentation quality in corn ear silage, the present invention provides a composite bacterial agent, and further provides the application of the composite bacterial agent in corn ear silage

Method used

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  • A kind of composite microbial agent and its preparation method and application

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Rhamnosus was activated and cultured at 34 °C for 18 h, inoculated into MRS medium, and cultured statically at 37 °C for 24 hours, so that the final number of viable bacteria reached 3.5×10 9 CFU / ml, to obtain Lactobacillus rhamnosus suspension;

[0022] The ethanol-resistant Pediococcus was activated and cultured at 34 °C for 18 h, inoculated into MRS medium, and cultured statically at 33 °C for 24 hours, so that the final number of viable bacteria reached 3.5×10 9 CFU / ml, to obtain ethanol-resistant Pediococcus suspension;

[0023] Lactobacillus plantarum was activated and cultured at 37 °C for 18 h, and the activated bacterial solution was inoculated into the MRS broth enrichment medium, cultured in the dark at 37 °C, and cultured on a constant temperature shaker at 150 rpm so that the final number of viable bacteria reached 5×10 10 CFU / ml, to obtain a suspension of Lactobacillus plantarum;

[0024] Lactobacillus Brucella was activated and cultured at 37 °C for 18 ...

Embodiment 2

[0034] Rhamnosus was activated and cultured at 34 °C for 18 h, inoculated into MRS medium, and cultured statically at 37 °C for 24 hours, so that the final number of viable bacteria reached 3.5×10 9 CFU / ml, to obtain Lactobacillus rhamnosus suspension;

[0035] After the ethanol-resistant Pediococcus was activated and cultured at 34 °C for 18 h, it was inoculated into MRS medium, and cultured statically at 33 °C for 24 hours, so that the final number of viable bacteria reached 3.5×10 9 CFU / ml, to obtain ethanol-resistant Pediococcus suspension;

[0036] The obtained Lactobacillus rhamnosus suspension and the ethanol-resistant Pediococcus suspension are mixed according to a volume ratio of 1:1.5 to prepare a composite bacterial agent;

[0037] Harvest the complete ear of corn with bracts in the milk ripening stage, and use a hammer feed mill to crush the fresh samples until the grains are broken, and add the above-mentioned compound bacterial agent, the dosage is 1×10 5 CFU / g...

Embodiment 3

[0039] Rhamnosus was activated and cultured at 34 °C for 18 h, inoculated into MRS medium, and cultured statically at 37 °C for 24 hours, so that the final number of viable bacteria reached 3.5×10 9 CFU / ml, to obtain Lactobacillus rhamnosus suspension;

[0040] The ethanol-resistant Pediococcus was activated and cultured at 34 °C for 18 h, inoculated into MRS medium, and cultured statically at 33 °C for 24 hours, so that the final number of viable bacteria reached 3.5×10 9 CFU / ml, to obtain ethanol-resistant Pediococcus suspension;

[0041] The obtained Lactobacillus rhamnosus suspension and the ethanol-resistant Pediococcus suspension are mixed according to a volume ratio of 1:1 to prepare a composite bacterial agent;

[0042] Harvest the complete ear of corn with bracts in the milk ripening stage, and use a hammer feed mill to crush the fresh samples until the grains are broken, and add the above-mentioned compound bacterial agent at a dosage of 1×10 5 CFU / g, mixed evenly, p...

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Abstract

The invention discloses a compound bacterial agent and its preparation method and application, belongs to the technical field of animal husbandry, relates to silage, and solves the problems of serious dry matter loss and low fermentation quality in corn cob silage. A composite microbial agent, which is composed of Lactobacillus rhamnosus and Pediococcus ethanol-resistant at a volume ratio of 1:1-1.5, Lactobacillus rhamnosus ZXY7, preservation number: CGMCC NO.11849, preservation date: 2015 On December 9, Pediococcus ethanolidurans NO.1, preservation number: CGMCC NO.11850, preservation date: December 9, 2015. The invention can significantly reduce the production of alcohols or volatile substances, has a high dry matter preservation rate, and can be widely used in other silages with high sugar content.

Description

technical field [0001] The invention belongs to the technical field of animal husbandry and relates to silage, in particular to a compound microbial agent, a preparation method thereof and an application in corn cob silage. Background technique [0002] Silage additives are an important part of the silage production process, which determines the quality of silage, and the quality of silage determines the quality of livestock products. [0003] About 50% of the silage in the domestic dairy farming industry is used in the form of TMR, and herbivores such as beef cattle and sheep are mainly fed separately. Whole plant corn silage is widely used in various dairy and breeding enterprises in my country. In order to achieve the purpose of effective feed utilization, secondary fermentation inhibitors are usually added to corn silage, acid agents and bacterial agents are added to alfalfa silage, etc. . Yeast can cause aerobic spoilage while relatively increasing the protein of silag...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N1/04A23K30/18C12R1/225C12R1/01
CPCA23K30/18C12N1/04C12N1/20
Inventor 许庆方玉柱熊乙欧翔马菱艺高占军
Owner SHANXI AGRI UNIV
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