Evaluation method of microorganism on influencing intestinal flora, and construction method of animal models with stable intestinal floras

An animal model, gut flora technology, applied in the biological field, can solve problems such as poor accuracy

Inactive Publication Date: 2018-06-12
深圳市百澳飞生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the evaluation of the influence of these microorganisms on the intestinal flora is mainly to use the reactor to simulate the intestinal environment, add microorganisms to the reactor and cultivate them, so as to detect t

Method used

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  • Evaluation method of microorganism on influencing intestinal flora, and construction method of animal models with stable intestinal floras
  • Evaluation method of microorganism on influencing intestinal flora, and construction method of animal models with stable intestinal floras
  • Evaluation method of microorganism on influencing intestinal flora, and construction method of animal models with stable intestinal floras

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0096] The construction process of the animal model of the present embodiment is as follows:

[0097] Ten 4-week-old C57BL / 6J germ-free mice were fed in a sterile isolator at 20°C, with a humidity of 70%, 12 hours of light and 12 hours of darkness, and fed with feces suspension at a concentration of 0.2g / mL liquid, and 0.5mL of feces suspension per kg of sterile mice was gavaged once a day for a total of 14 days; meanwhile, the sterile mice were fed with sterile feed every day, and each kg of Bacteria mice were fed with 2g of sterile feed each time, twice a day, for 14 days in total. Among them, the sterile feed was wheat, goat milk powder, soybean meal, peanut oil, vitamins, dry yeast, salt, calcium hydrogen phosphate and Citric acid, and the mass ratio of wheat, goat milk powder, soybean meal, peanut oil, vitamins, dry yeast, salt, calcium hydrogen phosphate and citric acid is 51:19.5:11:9.75:0.001:3.5:0.875:0.5:0.001. The feces of the sterile mice were collected on the 7th...

Embodiment 2

[0099] The construction process of the animal model of the present embodiment is as follows:

[0100] Ten 4-week-old C57BL / 6J germ-free mice were fed in a sterile isolator at 24°C, 40% humidity, 12 hours of light and 12 hours of darkness, and fed with feces suspension at a concentration of 0.5g / mL liquid, and 0.3mL of feces suspension per kg of sterile mice was administered orally once a day for a total of 14 days; at the same time, sterile feed was fed to the sterile mice every day, and no Bacteria mice were fed 5g of sterile feed each time, once a day, for a total of 14 days. Among them, the sterile feed was wheat, milk powder, soybean meal, corn oil, vitamins, yeast powder, salt, calcium hydrogen phosphate and citric acid, and the mass ratio of wheat, milk powder, soybean meal, corn oil, vitamins, yeast powder, salt, calcium hydrogen phosphate and citric acid is 52:20.5:12:10.75:1:4.5:1.875:1.5:0.525. The feces of the sterile mice were collected on the 7th day of gavage, t...

Embodiment 3

[0102] The construction process of the animal model of the present embodiment is as follows:

[0103] Ten 4-week-old C57BL / 6J germ-free mice were fed in a sterile isolator at 22°C, 55% humidity, 12 hours of light and 12 hours of darkness, and fed with feces suspension at a concentration of 0.5g / mL liquid, and 0.5mL of feces suspension per kg of sterile mice each time, twice a day for a total of 14 days; at the same time, the sterile mice were fed with sterile feed every Bacteria mice were fed 2g of sterile feed each time, twice a day, for 14 days in total, wherein the sterile feed was wheat, skimmed milk powder, soybean meal, soybean oil, vitamins, yeast powder, salt, calcium hydrogen phosphate and citric acid, and the mass ratio of wheat, skim milk powder, soybean meal, soybean oil, vitamins, yeast powder, salt, calcium hydrogen phosphate and citric acid is 51.5:20.0:11.5:10.25:0.5:4.0:1.375:1.0:0.125. The feces of the sterile mice were collected on the 7th day of gavage, th...

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Abstract

The invention relates to an evaluation method of a microorganism on influencing an intestinal flora, and a construction method of animal models with stable intestinal floras. The evaluation method comprises the steps of building the animal models with the stable intestinal floras; respectively feeding a sterile feed containing a microorganism to be tested and a sterile feed without the microorganism to be tested to the two groups of animal models, collecting faeces of the two groups of animal models after feeding to obtain a first faece and a second faece, determining and comparing contents ofthe intestinal floras in the first faece and the second faece; if a ratio of the logarithm sum of contents of bifidobacterium and bacterium lacticum to the logarithm sum of contents of enteric bacilli and enterococcus in the first faece is larger than a ratio of the logarithm sum of contents of bifidobacterium and bacterium lacticum to the logarithm sum of contents of enteric bacilli and enterococcus in the second faece, determining that the microorganism to be tested plays a role in promoting the intestinal flora. The evaluation method can be used for truly and accurately evaluating the influence of the microorganism on the intestinal flora.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for evaluating the influence of microorganisms on intestinal flora and a method for constructing an animal model with stable intestinal flora. Background technique [0002] Gut flora plays a vital role in the physiology, immunity, nutrition and digestion of organisms. Intestinal flora includes beneficial flora and harmful flora. Among them, beneficial bacteria can synthesize vitamins necessary for the growth and development of various organisms, participate in the metabolism of carbohydrates and proteins, and promote the absorption of mineral elements such as iron, magnesium, and zinc. Under normal circumstances, the beneficial bacteria and harmful bacteria in the intestinal tract of organisms are in a relatively balanced state. Once the intestinal flora is out of balance and harmful bacteria dominate, the harmful bacteria can convert the ingested nutrients into harmful sub...

Claims

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Application Information

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IPC IPC(8): C12Q1/689C12Q1/686C12Q1/04C12Q1/10A01K67/027C12R1/01C12R1/225
CPCA01K67/027A01K2227/105A01K2267/03C12Q1/686C12Q1/689
Inventor 向永生魏泓刘建华王玲玲
Owner 深圳市百澳飞生物技术有限公司
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