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Molecular marker closely linked to rice-taste-related QTLs and screening method thereof

A molecular marker, rice-flavored technology, applied in the field of genetic engineering, can solve the problem of lack of sufficient QTLs and molecular markers

Pending Publication Date: 2018-06-12
深圳盛宝联合谷物股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is still a lack of sufficient QTLs and molecular markers for rice palatability, including softness and hardness, sweetness after meals, etc.

Method used

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  • Molecular marker closely linked to rice-taste-related QTLs and screening method thereof
  • Molecular marker closely linked to rice-taste-related QTLs and screening method thereof
  • Molecular marker closely linked to rice-taste-related QTLs and screening method thereof

Examples

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Embodiment 1

[0046] Below taking embodiment 1 as example to specifically illustrate the technical scheme of the present invention:

[0047] 1. Construction of rice F4 generation segregation population

[0048] The breeding resource material SKO was selected as the male parent, and Wan 97 Xiang was selected as the female parent. SKO is an indica rice variety with a growth period of 100-105 days, a plant height of 110 cm to 120 cm, an upright plant type, light green leaves, and 12-15 leaves on the main stem. The rice quality test results are: brown rice rate 80%, polished rice rate 71%, whole polished rice rate 55%, grain length 7 mm. The amylose content is 17%, and the rice taste is very light, without sweetness. Wan 97 Xiang, this variety is an indica-type conventional rice, with a growth period of 85-90 days, a loose plant type, and light green leaves. The rice quality test shows that the brown rice rate is 78%, the polished rice rate is 68%, the whole polished rice rate is 55%, and th...

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Abstract

The invention discloses a molecular marker closely linked to rice-taste-related QTLs and a screening method thereof. A nucleotide sequence of the molecular marker is shown as a sequence list SEQ ID NO: 1. The screening method comprises the steps of constructing an F4 generation isolation group, extracting genomic DNA of male and female parents, F1 generation individuals and F4 generation individuals, constructing a genetic linkage map, using the genetic linkage map to locate the rice-taste-related QTLs, developing the molecular marker closely linked to the rice-taste-related QTLs, and preparing the molecular marker. On the basis of the technical scheme, the molecular marker closely linked to rice-taste sweet-returning genes can be screened out, the molecular marker can be used for rapidlydetecting whether or not rice contains the rice-taste sweet-returning genes, and rice breeding practice can be carried out simply and quickly and with high throughput.

Description

technical field [0001] The invention relates to the technical field of genetic engineering, in particular to a molecular marker closely linked to rice rice taste-related QTL and a screening method thereof. Background technique [0002] Rice is one of the most important food crops in my country. The planting area accounts for 30% of the area of ​​food crops, and the output accounts for 40% of the total food output. Rice is the staple food of more than 60% of the population in my country. Therefore, the improvement of rice production level has always been a major issue related to the development of agriculture and the stability of people's livelihood in our country. However, the current demand for rice production has continuously increased the quality of rice while pursuing yield in the past. Especially in my country, with the rapid economic development, people's living standards are constantly improving. Therefore, my country's rice production must further cultivate new vari...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 全志武林林林绿林李华
Owner 深圳盛宝联合谷物股份有限公司
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