Test paper for detecting progesterone of estrous female dog and preparation method of test paper

The technology of bitch progesterone and test paper is applied in the field of colloidal gold immunochromatography, which can solve the problems of complex detection method and inconvenient detection of bitch progesterone in estrus, and achieve the effects of avoiding pollution or infection, easy operation and high sensitivity.

Inactive Publication Date: 2018-06-12
NANCHANG POLICE DOG BASE MINIST OF PUBLIC SECURITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The present invention aims at the technical defects of the prior art, and provides a test paper for the detection of progesterone in estrous female dogs,

Method used

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  • Test paper for detecting progesterone of estrous female dog and preparation method of test paper
  • Test paper for detecting progesterone of estrous female dog and preparation method of test paper
  • Test paper for detecting progesterone of estrous female dog and preparation method of test paper

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Experimental program
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Effect test

Embodiment 1

[0036] 1. A test paper for progesterone detection in estrous female dogs and a preparation method thereof

[0037] 1.1 Preparation of colloidal gold

[0038] Colloidal gold is a negatively charged hydrophobic colloid solution that can be polymerized into gold particles of a certain size under the action of chloroauric acid under the action of a reducing agent, and is stable due to electrostatic interaction. The basic principle of immunocolloidal gold technology is that the surface of colloidal gold is negatively charged and electrostatically combined with the positively charged groups of protein molecules, and the complex formed will not affect the biological characteristics of the protein. The present invention selects trisodium citrate as a reducing agent to prepare colloidal gold, and the specific process is as follows: take 100mL of 0.01% chloroauric acid aqueous solution and heat it to boiling in a clean Erlenmeyer flask, quickly add 1.45mL of 1% trisodium citrate and kee...

Embodiment 2

[0080]A test strip for the detection of progesterone in female dogs in estrus, including a sample pad, a binding pad, a test line T, a quality control line C, a nitrocellulose membrane, absorbent paper and a PVC backing, wherein the nitrocellulose membrane is adhered to the PVC backing On the lining, a sample pad and a binding pad are attached to one end of the nitrocellulose membrane, and absorbent paper is attached to the other end, the detection line T and the quality control line C are located on the surface of the nitrocellulose membrane, and the sample pad, The distribution positions of binding pad, detection line T, quality control line C, and absorbent paper on the nitrocellulose membrane are sample pad, binding pad, detection line T, quality control line C, and absorbent paper. The detection line T The distance from the quality control line C is 0.7cm;

[0081] Heparin with a concentration of 15 mg / mL, sodium lycate with a concentration of 22 mg / mL, and ammonium sulfa...

Embodiment 3

[0091] A test strip for the detection of progesterone in female dogs in estrus, including a sample pad, a binding pad, a test line T, a quality control line C, a nitrocellulose membrane, absorbent paper and a PVC backing, wherein the nitrocellulose membrane is adhered to the PVC backing On the lining, a sample pad and a binding pad are attached to one end of the nitrocellulose membrane, and absorbent paper is attached to the other end, the detection line T and the quality control line C are located on the surface of the nitrocellulose membrane, and the sample pad, The distribution positions of binding pad, detection line T, quality control line C, and absorbent paper on the nitrocellulose membrane are sample pad, binding pad, detection line T, quality control line C, and absorbent paper. The detection line T The distance from the quality control line C is 0.7cm;

[0092] Heparin with a concentration of 15 mg / mL, sodium lycate with a concentration of 22 mg / mL, and ammonium sulf...

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Abstract

The invention provides test paper for detecting progesterone of an estrous female dog and a preparation method of the test paper. The technical scheme is based on a colloid gold immunochromatography and adopts a competition method, an improved progesterone antigen composition and goat (rabbit) anti-mouse IgG are fixed on a nitrocellulose membrane (NC) in a strip form, an anti-progesterone monoclonal antibody-colloid gold marker is fixed on a combination pad, when a sample to be detected is loaded to a test strip sample loading hole, the sample to be detected moves forwardly by virtue of the effect of a capillary tube, after the colloid gold marker on the combination pad is dissolved, the sample to be detected has inter-reaction and then is moved to an area for fixing an antigen or antibody, the sample to be detected is specifically combined with the colloid gold marker to be intercepted and accumulated onto a detection strip, and a developing result can be observed by eyes. The test paper is simple and rapid in detection, free from complicated operation skills and special devices and convenient to carry; and in addition, the test paper can realize semi-quantitative analysis for theprogesterone, is sensitive and accurate in result, and is more objective and effective for evaluating the estrous situation of the female dog.

Description

technical field [0001] The invention relates to the technical field of colloidal gold immunochromatography, in particular to a test paper for detecting progesterone in estrous female dogs and a preparation method thereof. Background technique [0002] In the dog breeding work, judging whether the female dog is in heat is an important prerequisite for the breeding work. The estrus identification of the bitch can effectively judge the estrus stage of the bitch, so as to determine the appropriate timing of mating, improve the conception rate and litter rate, and has important practical significance for improving the reproductive performance and breeding level of dogs. The traditional methods of estrus identification include external observation method, estrus test method, vaginal epithelial cell detection method, electrical resistance measurement method and so on. The external observation method requires inspectors to have rich theoretical and practical experience, and the acc...

Claims

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Application Information

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IPC IPC(8): G01N33/74G01N33/577G01N33/558G01N33/531
CPCG01N33/531G01N33/558G01N33/577G01N33/74G01N2333/575
Inventor 熊前李川武潘彩霞陈松昌陈文瑶吴衍叶俊华杨前勇李红王春亮余盼李强戴宗浩
Owner NANCHANG POLICE DOG BASE MINIST OF PUBLIC SECURITY
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