Application of akebia saponin D to preparation of medicines for promoting osteoarthritic cartilage repair
A technology of akebia saponin and osteoarthritis, applied in the field of biomedicine, can solve the problems of cartilage protection and effects that have not been reported, and achieve the effect of reducing articular cartilage damage
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Embodiment 1
[0024] Wistar rats were anesthetized with phenobarbital sodium (37.8 mg / kg), routinely prepared the skin to disinfect the knee joint of the left hind limb, took an incision on the medial side of the patella to expose the knee joint, dislocated the patella laterally after exposing the patella, and flexed the knee joint as far as possible before exposing For the cruciate ligament, the anterior cruciate ligament was cut under direct vision, the medial meniscus was removed, the bleeding was completely stopped, the patella was reset, and the joint cavity was closed layer by layer with No. 1 silk suture. Three days after operation, 200,000 units of penicillin was intramuscularly injected into each rat every day to prevent infection. In the sham operation control, the knee joint was entered in the same way, the patella was dislocated, the knee joint was flexed to expose the anterior cruciate ligament, and then the patella was reset to close the joint cavity. One week later, the opera...
Embodiment 2
[0028]When the mesenchymal stem cells grow to 90% confluence, rinse with PBS, digest with trypsin, take 5×10 5 Place the cells in a 15mL centrifuge tube, centrifuge at 500g for 5min, discard the supernatant, add chondrogenic differentiation medium for induction culture: DMEM, 10% FBS, 100nM dexamethasone, 50μg / mL vitamin C, 10ng / mL TGF -β3, 1% ITS (100X). In the treatment group, ASD (100 μM) was added at the same time as the induction solution, and the solution was changed every 3 days for 21 days of induction. The induced cell mass was washed with PBS, fixed with 4% paraformaldehyde for 24 hours, embedded in paraffin, 5 μm sections were dewaxed to water, and stained with 1% Alcian blue. Observe and take pictures under a light microscope, and compare the differences in cell differentiation in each group (see Figure 8 to Figure 15 ).
[0029] The result shows: normal group (see Figure 8 ): Mesenchymal stem cells are not stained with Alixinlan. induction group (see Figu...
Embodiment 3
[0032] Select 50 healthy Kunming mice, male, (20 ± 2) g, and be randomly divided into 5 groups, model group, positive drug group (aspirin tablets, 100 mg / kg), aketoside D (administration of 50, 150 mg / kg, respectively) , 450mg / kg) 3 dose groups. Each group was administered intragastrically for 6 consecutive days. 1 hour after the last administration, intraperitoneally inject 0.8% acetic acid solution, 0.1ml / 10g. Observe and record the number of times of writhing of mice in each group within 15 minutes, and compare the differences in the number of times of writhing of animals in each group (see Figure 16 ).
[0033] The results showed that the average number of writhing times in the model group was 38.86±4.33 times, the average number of writhing times in the positive drug aspirin group was 9.12±6.71 times, the average number of writhing times in the aketoside D 50mg / kg group was 32.28±6.49 times, and the average number of writhing times in the 150mg / kg group The body frequ...
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