Unlock instant, AI-driven research and patent intelligence for your innovation.

Optimized biomembrane living bacteria counting method

A technology for counting live bacteria and biofilm, which is applied in the field of optimized biofilm counting method, can solve the problems of decreased sensitivity of antibacterial agents, large differences among tested individuals, unfavorable screening work, etc., and achieves obvious dispersion effect and repeatability Strong, improve the effect of live bacteria count results

Active Publication Date: 2018-06-15
GUANGZHOU LIBY
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] (3) Strong tolerance and decreased sensitivity to antibacterial agents;
Clinical trials are a more realistic and objective way to evaluate the antibacterial efficacy of products against biofilms. The results are relatively reliable and representative, but there are also some obvious disadvantages: (1) It takes a long time, about 1 week (2) High cost; (3) The individual differences in the test are large; (4) High cooperation of the test individual is required
Preliminary formulation research of oral care products requires the screening of a large number of antibacterial raw materials with different concentrations, and clinical trials are not conducive to the screening of a large number of formulations of oral care products in the early stage

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Optimized biomembrane living bacteria counting method
  • Optimized biomembrane living bacteria counting method
  • Optimized biomembrane living bacteria counting method

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0091] Preparation method of dental plaque biofilm

[0092]The preparation mode of dental plaque biofilm involved in the present invention is as follows:

[0093] (1) Streak culture for each oral pathogenic bacteria on a TSA-supplemented medium plate in an oxygen-free atmosphere at a constant temperature of 36°C for 2 to 6 days, until a large number of bacterial lawns appear on the plate;

[0094] (2) Inoculate each cultured oral pathogenic bacteria into BHI-enriched medium, and culture them in an oxygen-free atmosphere at a constant temperature of 36°C for 1 to 3 days until the medium is cloudy, indicating that the culture of the bacterial suspension is mature ;

[0095] (3) Mix the mature bacterial suspension into the BHI enriched medium added with 0.2% adhesion promoter according to the volume ratio of 2% to 8%, and inoculate the TC in an oxygen-free atmosphere at a constant temperature of 36°C. In the small wells of the treated cell culture plate (24-well plate), a den...

Embodiment 1

[0218] Specifically include the following steps:

[0219] 1) Culture of dental plaque biofilm

[0220] According to the steps in the preparation example of the dental plaque biofilm model, the dental plaque biofilm was cultivated in the TC-treated cell culture plate. Calcium carbonate suspension was added as an adhesion promoter, and the incubation time was 16 hours.

[0221] 2) Antibacterial testing of oral care products using dental plaque biofilm

[0222] Draw TC to handle the bacterial suspension in the two small holes of the cell culture plate, and discard it; add 0.2mL~1mL oral care product samples of a certain concentration along the hole wall (the test sample solution in this embodiment is the test sample solution of the toothpaste sample) , keep for 3 minutes, draw the sample, and discard it; immediately add an equal volume of BHI-enriched medium to the small well along the wall of the hole, absorb the BHI-enriched medium, and discard it; then add it twice along the...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to the technical field of oral cavity nursing product antibacterial efficiency evaluation, and more specifically discloses an optimized biomembrane living bacteria counting method. According to the optimized biomembrane living bacteria counting method, a pancreatic enzyme solution is adopted for digestion dispersion of a dental plaque biomembrane model, an obtained biomembrane suspension liquid is subjected to ultrasonic dispersion at the same time; a living bacteria counting method is adopted for counting of bacteria number. The bacteria colony dispersion effect is obvious, the accuracy of living bacteria counting results is increased, and the obtained living bacteria number is more close to practical living bacteria number on biomembrane. In addition, the culture conditions of the dental plaque biomembrane model are optimized, the reliable dental plaque biomembrane model capable of increasing oral cavity nursing product antibacterial efficiency evaluation accuracy is obtained through culturing at certain culture conditions; a certain amount of a calcium carbonate suspending liquid is added as an adhesion promoter, so that the culture time of the dental plaque biomembrane model is shortened effectively without influencing the evaluation accuracy of subsequent oral cavity nursing product evaluation.

Description

technical field [0001] The invention relates to the technical field of live bacteria counting, in particular to an optimized method for counting live bacteria in biofilms. Background technique [0002] There are a variety of microorganisms living in the oral cavity, including bacteria, fungi, viruses, mycoplasma, protozoa, etc., and their existence forms include free state and aggregated state. Free state microorganisms are called planktonic microorganisms, and aggregated state microorganisms are mainly dental plaque biofilms. present in the human oral cavity. Compared with the free state, the microbial population in the form of biofilm has the following characteristics: [0003] (1) Microorganisms accumulate and colonize each other, with spatial and environmental diversity; [0004] (2) strong activity, able to survive harsh environments; [0005] (3) Strong tolerance and decreased sensitivity to antibacterial agents; [0006] (4) It can express a phenotype different fr...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12Q1/06C12N1/20C12R1/46C12R1/04C12R1/225C12R1/01
CPCC12N1/20C12Q1/06
Inventor 李淑钰江丹钟美邱振名黄亮张利萍
Owner GUANGZHOU LIBY