Application of isorhynchophylline to preparation of medicine with nerve protection effect

A technology of neuroprotection and isorynchophylline, applied in the field of natural medicinal chemistry, can solve problems such as reducing MAO-A activity, achieve good protective effects, expand blood vessels, and slow heart rate

Inactive Publication Date: 2018-06-22
SOUTH UNIVERSITY OF SCIENCE AND TECHNOLOGY OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, studies have shown that isorhynchophylline can significantly reduce the activity of MAO-A

Method used

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  • Application of isorhynchophylline to preparation of medicine with nerve protection effect
  • Application of isorhynchophylline to preparation of medicine with nerve protection effect
  • Application of isorhynchophylline to preparation of medicine with nerve protection effect

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] This example verifies the neuroprotective effect of isorhynchophylline through the following experimental steps

[0031] Dopaminergic neuron-specific toxin MPTP (1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine) 40 μg / ml treated zebrafish embryos 1-3 days after fertilization, 20 μg / mL Benzylmethynylamine (Deprenyl) was used as a positive control, 20 μM isorhynchophylline (indicated by Z2) was added, and the dopaminergic neuron specific marker gene dat was used as a probe for in situ hybridization to determine the results.

[0032] The result is as figure 1 (control group), figure 2 (MPTP group), image 3 (benzylmethynylamine group and MPTP mixed group) and Figure 4 (mixed group of isorhynchophylline Z2 and MPTP). From the comparison of the figures, it can be concluded that Z2 can effectively reverse the death of dopaminergic neurons induced by MPTP. It is generally believed that Parkinson's disease is caused by the death of dopaminergic neurons, and dat is the marker...

Embodiment 2

[0034] In this example, the following experiments were performed to detect dopaminergic neurons by in situ hybridization with dat probes, and to observe the changes in the number of dopaminergic neurons before and after drug administration in zebrafish embryos 3 days after fertilization.

[0035] A, adding 0.1% PTU to the embryos of the control group to remove the pigment;

[0036] B, MPTP treatment of embryos for 48 hours;

[0037] C, adding the positive control drug Deprenyl and MPTP to co-treat the embryo for 48 hours;

[0038] D. Embryos treated with MPTP and Z2.

[0039] From the experiment, the normal expression of diencephalic dopaminergic neurons in group A; the death of a large number of diencephalic dopaminergic neurons in group B; the increase of dopaminergic neurons in group C compared with group B; Both elements have recovered significantly, and the protection effect of Z2 is better.

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Abstract

The invention provides application of isorhynchophylline to preparation of a medicine with a nerve protection effect. The medicine with the nerve protection effect, provided by the invention, can effectively relieve the toxic effect of the neurotoxin MPTP to the dopaminergic neuron and has a protection effect on the dopaminergic neuron. Aiming at the common effects of the isorhynchophylline on reducing blood pressure, expanding blood vessels and slowing down the heart rate, the invention develops new application of the isorhynchophylline and provides a theoretical foundation and an experimental basis for further researching the neuropharmacology of the traditional Chinese medicinal material uncaria; furthermore, the isorhynchophylline provides a new idea and possibility for development ofmedicines for treating Parkinson's disease, medicines for resisting depression and medicines for treating Alzheimer's disease; and particularly, the isorhynchophylline has especially prominent effectsby serving as a medicine for treating Parkinson's disease.

Description

technical field [0001] The invention belongs to the field of natural medicinal chemistry, and relates to the application of isorhynchophylline in the preparation of medicines with neuroprotective effect. Background technique [0002] Uncaria is an evergreen vine of the Rubiaceae Uncaria genus, a climbing shrub, with square columnar stems and branches that are slender and hairless, papery leaves that are oval, and flower heads. Uncaria is used as medicine with hooked stems and branches. It has the functions of clearing away heat, calming the liver, relieving wind and relieving convulsions. Modern pharmacological experiments have also proved that Uncaria has antihypertensive effect, so it has high medicinal value. Previous studies have shown that Uncaria decoction, ethanol extract, total alkaloids and rhynchophylline, no matter to anesthetized animals or non-anesthetized animals, normal animals or hypertensive animals, and regardless of intravenous injection or intragastric ad...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/438A61P25/28A61P25/24A61P25/16
CPCA61K31/438
Inventor 杨选军仲寒冰
Owner SOUTH UNIVERSITY OF SCIENCE AND TECHNOLOGY OF CHINA
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