Nanometer mimic enzyme based on colorimetric fluorescence dual channels and application thereof to analysis detection

Abstract

The invention discloses a carbon point-protoheme based analogue peroxide enzyme composite nanometer material, and builds a method for visually detecting hydrogen peroxide, glucose and xanthine via visual and fluorescence dual channels by using the nanometer material. According to the method, a stable red color development product is formed by catalyzing hydrogen peroxide phenol oxide and 4-amino-antipyrine by carbon point-protoheme analogue enzymes; the maximum absorption peak of the color development product is 505nm; meanwhile, the fluorescence of the reaction liquid of the carbon point-protoheme nanometer material in a position with the excitation wavelength being 480 nm and the emission spectrum being 540nm generates quenching. Therefore the light absorption degree of color developmentproduct in the 505nm position and the fluorescence quenching signals of the color development product in the 540nm emission spectrum position respectively realize the linear relationship with hydrogen peroxide, glucose and xanthine in a range of 1.7*10<-7> to 2*10<-4> M, 1.7*10<-7> to 1.3*10<-4> M and 3.3*10<-6> to 3.3*10<-5>M.

Description

technical field [0001] The invention belongs to the field of analytical chemistry technology and nanotechnology, and in particular relates to a nanozyme with visible and fluorescent dual channels for visual detection. The simulated enzyme is a composite material of carbon dots and heme, which catalyzes the oxidation of hydrogen peroxide Phenol and 4-aminoantipyrine form a stable red color product, and establish a dual-channel analytical method for the detection of hydrogen peroxide, glucose and xanthine. Background technique [0002] Natural enzymes have the advantages of high catalytic efficiency, strong substrate selection specificity, and a wide range of catalytic reactions. However, natural enzymes also have obvious defects in practical applications, such as high production costs, harsh use conditions, and expensive prices. The key factors restricting the application of natural enzymes in production practice, so the development and application of artificial enzymes have ...

AI Technical Summary

Problems solved by technology

However, most of the nano-mimetic enzymes use a single UV-visible visualization analysis method, but the single signal is easily interfered by the substrate, resulting in unreliable detection results; and the dual-channel visualization analysis method based on UV-visible and fluorescence can not only The concentration of the target substance can be determined by comparing or measuring the color depth of the colored substance solution, and the detected substance can also be identified by the change of the fluorescent signal

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