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Detection probe as well as preparation method and application thereof

A technology for detecting probes and probes, applied in the field of nanomaterials, can solve problems such as expensive instruments and equipment, complex operation processes, etc., and achieve the effects of easy operation, broad application prospects, and efficient and convenient detection.

Active Publication Date: 2018-07-06
SOUTH UNIVERSITY OF SCIENCE AND TECHNOLOGY OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the sensitivity of these methods can meet the requirements, they usually require expensive instruments and complex operating procedures, which prevents them from being widely used in daily life.

Method used

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  • Detection probe as well as preparation method and application thereof
  • Detection probe as well as preparation method and application thereof
  • Detection probe as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Mix gold nanoparticles 1 with a diameter of 10 nm and nucleotide chains 2 with sulfhydryl groups at a concentration ratio of 1:200, add 0.1M NaCl, 5mM NaHPO 4 , stand still for 16 hours, then centrifuge at 10,000 rpm for 10 minutes, and remove the supernatant;

[0052] Mix graphene quantum dots 3 with a diameter of 5 nm and nucleotide chains 4 with amino groups at a concentration ratio of 1:200, add 27 mM EDC solution, mix for 2 minutes and then stand still for 1 hour;

[0053] The thiol-modified DNA nucleotide chain is:

[0054] 5'- / 3ThioMC3-D / CGATAACTCACTATrAGGAAGAGATG-3';

[0055] The DNA nucleotide chain after amino modification is:

[0056] 3'-TGAGTGATAAAGCTGGCCGAGCCTTTTCTCTAC / 5AmMC6 / -5';

[0057] Among them, the transmission electron microscope images of nanomaterial substances (gold nanoparticles and graphene quantum dots) are as follows figure 1 shown;

[0058] Place the above-mentioned graphene quantum dots and gold nanoparticles that are combined with nuc...

Embodiment 2

[0063] Add different metal ions to the detection system in Example 1 to detect the fluorescence signal intensity, and the fluorescence intensity comparison is shown in Figure 6 shown;

[0064] Depend on Figure 6 It can be seen that the probe provided by the present invention can specifically detect the concentration of lead ions, but is insensitive to other metal ions and has no detection function.

Embodiment 3

[0066] Mix gold nanoparticles 1 with a diameter of 7nm and nucleotide chains 2 with sulfhydryl groups at a concentration ratio of 1:180, add 0.05M NaCl, 3mM NaHPO 4 , stand still for 13h, then centrifuge at 10000rpm for 10min, and remove the supernatant;

[0067] Mix graphene quantum dots 3 with a diameter of 3nm and nucleotide chains 4 with amino groups at a concentration ratio of 1:180, add 25mM EDC solution, mix well for 1min and then stand still for 0.5h; combine the above with nucleotide chains The graphene quantum dots and gold nanoparticles placed on the fluorescence spectrometer, under the irradiation of 370nm excitation light, carry out spectral signal detection combined nucleotides, and then add different concentrations of lead ions, the results are similar to those in Example 1 and Example 2 , which will not be repeated here.

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Abstract

The invention provides a detection probe as well as a preparation method and application thereof. The detection probe comprises a probe A and a probe B, wherein the probe A is combined with a graphenequantum dot; the probe B is combined with gold nanoparticles; the nucleotide sequence of the probe A is as shown in SEQ ID NO:1; the nucleotide sequence of the probe B is as shown in SEQ ID NO:2; since the graphene quantum dot has an intense fluorescent signal in the position of the wavelength of 450 nm under radiation of excited light with the wavelength of 390 nm, the graphene quantum dot and agold nano material can be adsorbed together by virtue of the interaction of a DNA (Deoxyribonucleic Acid) nucleotide chain, and then the fluorescent signal of the graphene quantum dot can be quenched; and since lead ions have a shearing function on the nucleotide chain on the graphene quantum dot, the graphene quantum dot can be separated from the gold nanoparticles, the fluorescent signal on thegraphene quantum dot can be recovered, and the concentration of the lead ions can be detected according to the intensity of the fluorescent signal. The detection probe provided by the invention can be adopted to detect the concentration of lead ions, is efficient and concise and has wide application prospects.

Description

technical field [0001] The invention relates to the technical field of nanomaterials, in particular to a detection probe and its preparation method and application. Background technique [0002] The harm of heavy metal ions to the human body and the environment has attracted more and more people's attention and research, especially lead ions, which can cause brain memory loss, emotional instability, cardiovascular disease, and muscle paralysis in young children. Lead ions not only exist in plants and animals, but also in our environment, it is a non-degradable substance. The U.S. Environmental Protection Agency points out that the maximum lead ion content in human drinking water is 15μg / L or 72nM. [0003] Quantum Dots are composed of a limited number of atoms and are quasi-zero-dimensional materials, that is, the size of the quantum dots is at the nanometer level in three dimensions, and the appearance is like a spherical or spherical object, and the movement of the electr...

Claims

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Application Information

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IPC IPC(8): G01N21/64
CPCG01N21/643
Inventor 罗丹牛小方
Owner SOUTH UNIVERSITY OF SCIENCE AND TECHNOLOGY OF CHINA
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