Preparation method of 4-tertiary butyl calix(4)arene modified electrode applied to electrochemical method to recognize amino acid enantiomer

A technology of tert-butyl cup and modified electrode, which is applied in the fields of electrochemical analysis and biology, and achieves the effects of environmental protection and pollution-free preparation process, simple and easy method, and efficient identification.

Inactive Publication Date: 2018-07-06
CHANGZHOU UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, in the published literature so far, the use of 4-tert-butylcalix[4]aren

Method used

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  • Preparation method of 4-tertiary butyl calix(4)arene modified electrode applied to electrochemical method to recognize amino acid enantiomer
  • Preparation method of 4-tertiary butyl calix(4)arene modified electrode applied to electrochemical method to recognize amino acid enantiomer
  • Preparation method of 4-tertiary butyl calix(4)arene modified electrode applied to electrochemical method to recognize amino acid enantiomer

Examples

Experimental program
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Example Embodiment

[0022] Example one:

[0023] The preparation of 4-tert-butylcalix[4]arene modified electrode for electrochemical recognition of tryptophan enantiomers involves the following steps:

[0024] (1) Grind 4-tert-butylcalix[4]arene into powder and ultrasonically disperse it in ultrapure water to prepare 3mM 4-tert-butylcalix[4]arene dispersion. Take 10 μL drop-coated on the surface of the glassy carbon electrode and dry at room temperature to obtain a 4-tert-butylcalix[4]arene modified electrode.

[0025] (2) The experiment uses a three-electrode system. The 4-tert-butylcalix[4]arene modified electrode is the working electrode, the platinum electrode is the counter electrode, and the saturated calomel electrode is the reference electrode. The three-electrode system is immersed in the prepared In 1.0mM L- / D-tryptophan solution, after standing for 90s, the differential pulse test is carried out in the potential range of 0.4~1.2V at a sweep rate of 8mV / s. After each measurement, the modified...

Example Embodiment

[0026] Embodiment two:

[0027] The preparation of 4-tert-butylcalix[4]arene modified electrode for electrochemical recognition of tyrosine enantiomer includes the following steps:

[0028] (1) Grind 4-tert-butylcalix[4]arene into powder and ultrasonically disperse it in ultrapure water to prepare 3mM 4-tert-butylcalix[4]arene dispersion. Take 10μL drop-coated on the surface of the glassy carbon electrode and dry at room temperature to obtain 4-tert-butylcalix[4]arene modified electrode.

[0029] (2) The experiment uses a three-electrode system. The 4-tert-butylcalix[4]arene modified electrode is the working electrode, the platinum electrode is the counter electrode, and the saturated calomel electrode is the reference electrode. The three-electrode system is immersed in the prepared In 1.0mM L- / D-tyrosine solution, after standing for 90s, the differential pulse test is performed in the potential range of 0.4~1.2V at a sweep rate of 8mV / s, and the modified electrode is immersed in p...

Example Embodiment

[0030] Embodiment three:

[0031] Different concentrations of 4-tert-butylcalix[4]arene modified electrodes to recognize tryptophan enantiomers involves the following steps:

[0032] (1) Grind 4-tert-butylcalix[4]arene into powder and ultrasonically disperse it in ultrapure water to prepare dispersions of 4-tert-butylcalix[4]arene with different concentrations. The experimental concentration is 1.0~5.0 mM. Take 10 μL of 4-tert-butylcalix[4]arene dispersions with different concentrations and apply dropwise to the surface of the glassy carbon electrode and dry at room temperature to obtain 4-tert-butylcalix[4]arene modified electrode.

[0033] (2) The experiment adopts a three-electrode system, with different concentrations of 4-tert-butylcalix[4]arene modified electrode as the working electrode, platinum electrode as the counter electrode, and saturated calomel electrode as the reference electrode. Dip into the prepared L- / D-tryptophan solution, let it stand for 90s, and perform a d...

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Abstract

The invention relates to a preparation method of a 4-tertiary butyl calix(4)arene modified electrode applied to an electrochemical method to recognize an amino acid enantiomer. The preparation methodcomprises the following steps of preparing a 4-tertiary butyl calix(4)arene modified electrode and recognizing the amino acid enantiomer through the electrochemical method. The preparation method disclosed by the invention has the beneficial effects that the 4-tertiary butyl calix(4)arene modified electrode preparation method is simple, convenient, easy and practical, a preparation process achieves environmental friendliness and has no pollution, and furthermore, the modified electrode can recognize the amino acid enantiomer in high efficiency.

Description

technical field [0001] The invention relates to a preparation method of a 4-tert-butylcalix[4]arene modified electrode used for electrochemically identifying amino acid enantiomers, and belongs to the fields of electrochemical analysis and biotechnology. technical background [0002] Amino acids are the basic units of biologically functional macromolecular proteins, and proteins are the main bearers of life activities. The molecular structures of enantiomers in different configurations are mirror images of each other but cannot be superimposed. They have the same physical properties but exhibit completely different pharmacological activities, metabolic processes and toxicological effects in vivo. Therefore, the identification and analysis of amino acid enantiomers is established. Methods play a very important role in life sciences, medicinal chemistry, and human health. At present, there are many technologies for chiral recognition of amino acids. Electrochemical methods ha...

Claims

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Application Information

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IPC IPC(8): G01N27/327
CPCG01N27/3277
Inventor 孔泳杨嘉佩谭文胜
Owner CHANGZHOU UNIV
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