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Tissue culture medium of monstera deliciosa seeds

A technology of tissue culture medium and monstera, which is applied in horticulture, botany equipment and methods, gardening tools/equipment, etc. It can solve the problems of increasing the thickness of buds, shortening the time of seedling cultivation, and low reproduction coefficient, so as to promote the health of seedlings Strong, improve the survival rate, promote the effect of plant growth

Inactive Publication Date: 2018-07-20
平南县德湖种养农民专业合作社
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the natural state, Monstera mainly relies on seeds for breeding, but the reproduction coefficient is low. In addition, it is obviously difficult to artificially raise seedlings by taking seeds. Therefore, it is necessary to provide a method that can make Monstera seeds as provenance, shorten the seedling time, and ensure It is imminent to cultivate the survival rate of buds after budding, increase the thickness of buds, and ensure the dark green color of buds after budding

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] A kind of monstera seed tissue culture medium provided in this embodiment is formed by mixing the basal culture solution and the mother solution, and their volume ratio is 4:0.05; wherein, each liter of the mother solution mainly contains: 10 mg of hymexazol, 10 mg of Sodium nitrophenolate, 20mg of sodium α-naphthalene acetate, 0.3mg of niacin, 6mg of microcystin, 0.2mg of potassium iodide and 20mg of diethyl hexanoate; each liter of basic culture medium mainly contains: 25mg of hydrolyzed potato dregs solution, 5mg of glucose, 10mg of agar, 5mg of coconut milk, 4mg of tomato juice, 5mg of hydrolyzed milk protein, 1mg of corn endosperm, 50mg of inositol and 1mg of gibberellin; the potato dregs hydrolyzate is mainly obtained through the following processes : a. Drying and pulverizing the potato residues, then adding ionic liquid until the mass concentration of the potato residues is 5%, then stirring for 60 minutes at 90°C and 80r / min to obtain a potato residues solution,...

Embodiment 2

[0021] A kind of monstera seed tissue culture medium provided in this embodiment is formed by mixing the basic culture solution and the mother solution, and their volume ratio is 5:0.07; wherein, each liter of the mother solution mainly contains: 12 mg of hymexazol, 15 mg of Sodium nitrophenolate, 35mg of sodium α-naphthalene acetate, 0.4mg of niacin, 9mg of microcystin, 0.3mg of potassium iodide and 35mg of diethyl hexanoate; each liter of basic culture medium mainly contains: 30mg of hydrolyzed potato dregs solution, 7mg of glucose, 15mg of agar, 7mg of coconut juice, 6mg of tomato juice, 7mg of hydrolyzed milk protein, 3mg of corn endosperm, 65mg of inositol and 2mg of gibberellin; the hydrolyzate of potato dregs is mainly obtained through the following processes : a. Dry and pulverize potato residues, then add ionic liquid until the mass concentration of potato residues is 7%, then stir for 90 minutes at 100°C and 100r / min to obtain a potato residues solution, then add pota...

Embodiment 3

[0024]A kind of monstera seed tissue culture medium provided in this embodiment is formed by mixing the basic culture solution and the mother solution, and their volume ratio is 6:0.07; wherein, each liter of the mother solution mainly contains: 15 mg of hymexazol, 20 mg of Sodium nitrophenolate, 50mg of sodium α-naphthalene acetate, 0.5mg of niacin, 12mg of microcystin, 0.4mg of potassium iodide and 50mg of diethyl hexanoate; each liter of basic culture medium mainly contains: 38mg of hydrolyzed potato dregs liquid, 10mg of glucose, 20mg of agar, 10mg of coconut juice, 8mg of tomato juice, 8mg of hydrolyzed milk protein, 4mg of corn endosperm, 50-80mg of inositol and 3mg of gibberellin; the main process of potato dregs hydrolyzate is as follows Process to obtain: a. Dry and pulverize potato residues, then add ionic liquid until the mass concentration of potato residues is 9%, and then stir for 120 minutes under the conditions of 120°C and 120r / min to obtain a solution of potat...

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Abstract

The invention provides a tissue culture medium of monstera deliciosa seeds and relates to the technical field of tissue culture. The culture medium provided by the invention is prepared by mixing a basic culture solution and a mother solution; the volume ratio of the basic culture solution to the mother solution is (4 to 6) : (0.05 to 0.09). According to the culture medium provided by the invention, potato dreg is utilized in the basic culture solution so that the dosage of culture medium raw materials including sucrose and the like in a traditional technology is reduced and the preparation cost of the culture medium is reduced; furthermore, a mother solution formula is added, so that seedling strengthening and rooting can be rapidly promoted, and the rooting rate reaches 90 percent or more; the average root number of the plants subjected to the seedling strengthening and rooting reaches 20 to 25.

Description

【Technical field】 [0001] The invention relates to the technical field of tissue culture, in particular to a monstera seed tissue culture medium. 【Background technique】 [0002] Monstera, (scientific name: Monstera deliciosa). Also known as "Penglai banana", "wire grass", evergreen vines. The stem is strong; the young leaves are heart-shaped without holes, and become broadly ovate and pinnately parted when they grow up; there are oval perforations between the veins, and the leaves are long-stalked and dark green. Spatha inflorescence, spathe boat-shaped, blooms in November, pale yellow. There are brown aerial roots on the stem, which are shaped like electric wires, so it is named "wire grass"; the leaves are oval, and there are many oblong holes and deep cracks scattered between the feathery veins in the shape of tortoise shells. The tortoise shell pattern, the stem is knotted like a bamboo stem, hence the name "Monstera". Spadix, the whole flower shape is like a "table l...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001
Inventor 覃伟杰
Owner 平南县德湖种养农民专业合作社
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