Antigen-loaded water-soluble aminated β-1,3-d-glucan nanoparticles and its preparation method and application
A nanoparticle and dextran technology, applied in the field of biomedical materials, can solve the problems of limited particle size and disadvantages of yeast dextran, and achieve the effect of maintaining structure and immunogenicity, increasing titer and maintaining stability
Active Publication Date: 2020-07-14
SUN YAT SEN UNIV
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Embodiment 1
[0068] Example 1 Preparation of water-soluble aminated β-1,3-D-glucan nanoparticles loaded with antigen
[0069] 1. Preparation of water-soluble aminated β-1,3-D-glucan
[0070] (1) Weigh 5g of β-1,3-D-glucan with a purity of 70% to 80%, pour it into a flask, add 200mL of formic acid, stir and reflux at 90°C for 4h; Cool to room temperature and stir, add absolute ethanol according to the volume ratio of formic acid and ethanol 1:4, precipitate out, centrifuge, wash the precipitate 6 times with absolute ethanol, and then freeze-dry. Pour 2 g of the freeze-dried sample into a flask, add 200 mL of deionized water, and reflux with magnetic stirring at 90°C for 4 hours; then rotatively evaporate the deionized water to a small amount, cool to room temperature, and mix according to the volume of deionized water and ethanol 1:4 After adding absolute ethanol, a precipitate precipitated, centrifuged, and the precipitate was freeze-dried to obtain purified β-1,3-D-glucan.
[0071] (2) ...
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Abstract
The invention discloses antigen-loaded water-soluble aminated beta-1,3-D-glucan nanoparticles as well as a preparation method and application thereof. The nanoparticles are prepared from the followingraw materials in parts by weight: 3.5 to 5.5 parts of water-soluble aminated beta-1,3-D-glucan, 0.06 to 1.19 parts of an ion crosslinking agent and 0.3 to 0.7 part of antigen protein, wherein the ioncrosslinking agent is a low molecular weight anionic surfactant. The grain diameter of the antigen-loaded water-soluble aminated beta-1,3-D-glucan nanoparticles provided by the invention is relatively small and is 100 to 1200nm; the antigen-loaded water-soluble aminated beta-1,3-D-glucan nanoparticles have good water solubility and the encapsulation efficiency of the antigen protein is high and can reach 86.5 percent at maximum; the stability of a structure and immunogenicity of the antigen protein can be kept, and the antigen protein can be continuously released; the continuous releasing time of the protein exceeds 125h and the releasing rate within 5min is lower than 40 percent; the titer of IgG1 and IgG2a in animal bodies can be remarkably improved. Meanwhile, the preparation method ofthe nanoparticles is simple, reaction conditions are moderate, and the reaction efficiency is relatively high.
Description
technical field [0001] The invention relates to the field of biomedical materials, more specifically, to a water-soluble aminated β-1,3-D-glucan nanoparticle loaded with an antigen and its preparation method and application. Background technique [0002] β-1,3-D-glucan mainly exists in the cell walls of bacteria, fungi, yeast and seaweed. There are two main aspects of related research, one is the study of the immunomodulatory activity of zymosan, and the other is the study of edible mushroom Studies on the antitumor activity of polysaccharides. Although the above studies are aimed at different raw materials, with the separation of their active components, the focus is on β-1,3-D-glucan. It has been found that β-1,3-D-glucan has many physiological activities, such as: immunomodulatory activity against infectious diseases and tumors; stimulation of hematopoietic function and protective effect after radiation; protection of tissues and organs from oxidative damage; Hyperchole...
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IPC IPC(8): A61K9/51A61K47/36A61K39/39
CPCA61K9/5161A61K39/39
Inventor 容敏智金静维章明秋
Owner SUN YAT SEN UNIV