Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Clostridium acetobutylicum and application thereof

A technology of Clostridium acetobutylicum and butanol, which is applied in the direction of bacteria, microorganisms, biofuels, etc., and can solve the problem of weak inhibitory ability

Active Publication Date: 2018-07-27
NANJING UNIV OF TECH
View PDF8 Cites 9 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Purpose of the invention: In order to solve the problem that the existing Clostridium acetobutylicum is weak in tolerance to acids, aldehydes and phenolic inhibitors, the present invention provides a Clostridium acetobutylicum, and the present invention further provides acetobutylic acid Application of Clostridium in Fermentative Production of Butanol

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Clostridium acetobutylicum and application thereof
  • Clostridium acetobutylicum and application thereof
  • Clostridium acetobutylicum and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1 Clostridium acetobutylicum LJ4 bacterial strain is isolated and screened in a screening plate containing furfural, 5-hydroxymethylfurfural, ferulic acid, p-coumaric acid, and vanillin each 0.5g / L

[0034] Weigh 1g of the soil sample taken from Pearl Spring soil, dilute it with normal saline, and pipette 200μL onto a plate containing 0.5g / L each of furfural, 5-hydroxymethylfurfural, ferulic acid, p-coumaric acid, and vanillin , and cultured anaerobically at 37°C for 192h. The grown colonies were streaked and purified for 5 generations, and the strain Clostridiumacetobutylicum LJ4 with strong tolerance to lignocellulose hydrolyzate inhibitors was screened out.

[0035] The medium formula of the above screening plate is NaCl 1g / L, K 2 HPO 4 0.75g / L, KH 2 PO 4 0.75g / L, yeast powder 3g / L, CaCl 2 2H 2 O 0.015g / L, FeCl 2 4H 2 O 1.5g / L, KCl 0.3g / L, furfural, 5-hydroxymethylfurfural, ferulic acid, p-coumaric acid, vanillin 0.5g / L each, adjust the pH to 4.5-6....

Embodiment 2

[0036] Example 2 Identification of Clostridium acetobutylicum LJ4 and its growth characteristics

[0037] 16S rDNA identification of LJ4:

[0038]Using primers 27F: 5'-AGAGTTTGATCCTGGCTCAG-3' and 1492R: 5'-TACCTTGTTACGACTT-3' to amplify the 16S rDNA of strain LJ4, connect it to the cloning vector pMD19T by T / A cloning, and construct the recombinant cloning vector pMD19T-16S, Transform it into the cloning host strain Escherich coli DH5α to obtain the recombinant microorganism Escherichcoli DH5α (pMD19T-16S), sequence the exogenous fragment of the obtained recombinant microorganism, compare the 16S rDNA sequence with the NCBI database, and identify the strain LJ4 at the molecular level For the genus Clostridium acetobutylicum, the nucleotide sequence of its 16SrDNA is shown in SEQ ID NO: 1 in the sequence listing.

[0039] Growth and metabolic characteristics of LJ4:

[0040] Strain LJ4 can grow well at 37°C, grow well at pH 4.5-6.5, and grow best at pH 5.5. LJ4 is in the log...

Embodiment 3

[0041] Example 3 Clostridium acetobutylicum C.acetobutylicum LJ4 growth OD and butanol production under different concentrations of furfural

[0042] Strain LJ4 can grow in the medium containing furfural, and at the furfural concentration of 1g / L, 2g / L, 3g / L, 4g / L, 5g / L, 8g / L, the growth status and fermentation of the bacteria LJ4 characteristics as an example.

[0043] Pick a single colony of strain LJ4 from the plate and inoculate it into 5mL liquid medium, at 37°C, 120r min -1 Cultivate for 48 hours, then inoculate into the fermentation medium with an inoculum size of 5% (v / v), 37°C, 120r·min -1 Shake the culture, adjust the pH to 5.5 every 24 hours, after 192 hours of fermentation, see the strain growth OD and butanol production figure 1 and figure 2 .

[0044] At 1g / L, 2g / L, 3g / L, 4g / L furfural concentrations, although the lag period of the bacterial strain LJ4 was extended, the growth condition was good, and the control butanol output without inhibitors was 12g / L. ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a clostridium acetobutylicum. The clostridium acetobutylicum has a strain number of LJ4 and is preserved in China Center for Type Culture Collection with a preservation numberof CCTCCNO: M 2017754. The invention further provides application of the clostridium acetobutylicum LJ4 to butanol fermentation production. The strain LJ4 can make good use of virus-carrying lignocellulose hydrolysate, when in absolute lignocellulose hydrolysate, can produce 13.9 g / L of butanol through fermentation, and when in 50% lignocellulose hydrolysate, can reach a maximum yield and produceup to 15.2 g / L of butanol, thereby inferring that the lignocellulose hydrolysate is complex in composition and contains substance capable improving the yield of butanol and meanwhile the concentrationof inhibitor is lower than the tolerance degree of the strain.

Description

technical field [0001] The invention relates to butanol-producing bacteria, in particular to Clostridium acetobutylicum and application thereof. Background technique [0002] Butanol is an important C4 platform compound, which has been widely used in the manufacture of various fine chemicals; and as an important new biofuel with great potential, it has been valued. The United Nations International Energy Agency has listed bio-butanol as a Second generation biofuels. As a fuel, butanol has the advantages of high energy density, direct use in internal combustion engines, and convenient transportation. In today's increasingly severe energy crisis, butanol has broad development prospects as a fuel. In 2007, the apparent consumption of butanol in my country has reached 740,000 tons, and it is estimated that the domestic demand for butanol will reach 1 million tons in 2010. Since domestic production cannot meet the demand, my country is already the world's largest importer of bu...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N1/20C12P7/16C12R1/145
CPCC12P7/16C12R2001/145C12N1/205Y02E50/10
Inventor 信丰学刘洁姜岷董维亮章文明马江锋周杰
Owner NANJING UNIV OF TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com