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A kind of drug extraction method of etomidate and fentanyl in whole blood during operation

A technology of etomidate and drugs, which is applied in the field of drug extraction of etomidate and fentanyl in intraoperative whole blood, can solve problems such as difficult component analysis, unstable components, and complexity, and achieve the effect of improving detection sensitivity

Active Publication Date: 2020-07-10
李恩有 +2
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AI Technical Summary

Problems solved by technology

[0004] Since whole blood is the direct output of human and animal individuals, it is often difficult to analyze its components due to its instability and complex components

Method used

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  • A kind of drug extraction method of etomidate and fentanyl in whole blood during operation
  • A kind of drug extraction method of etomidate and fentanyl in whole blood during operation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Such as figure 1 , extract blood sample 1ml to 5ml sample vial (select bottom glass centrifuge tube for sample vial), add 1.5ml chloroform and 1.5ml monochlorobutane to it, solvent is positioned at lower floor; The mouth is inclined upwards, so that the inclination angle of the central axis of the sample bottle is kept at 45o from the horizontal plane, and rotate along the central axis of the bottle body for 1min at a rotation speed of about 100rmp. Then use a disposable syringe treated with heparin on the inner wall to insert into the bottom of the sample bottle to extract the extraction solvent layer as the extraction solution for the next step of filtration operation, and take a syringe filter (Φ13, 0.2 um), the filtrate was used for subsequent use.

Embodiment 2

[0033] Take 50 ul of the solution to be tested prepared in Example 1, and enter the thermal analysis analyzer of the ion mobility spectrometry blood drug concentration analyzer. In the positive ion mode of photoionization ion mobility spectrometry, the detection signal of etomidate was at 5.12ms; the detection signal of sufentanil was at 5.60ms and 6.52ms. The respective signal intensities are brought into the fitted standard curve equation to analyze and calculate the drug concentrations of etomidate and fentanyl in whole blood, such as figure 2 shown.

[0034] The invention discloses a method for extracting mixed drugs from intraoperative whole blood samples used for ion mobility spectrometry analysis. The method is simple, fast and has good repeatability in preparing samples. The whole blood sample extraction method includes a whole blood sample emulsification reaction generation device and method, a mixed solvent extraction mixed medicine method and an extraction liquid ...

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Abstract

The invention discloses a mixed medicine extraction method in an intraoperative whole blood sample for ion migration spectrum analysis. The method is simple and fast; the prepared sample has good repeatability. The whole blood sample extraction method comprises a whole blood sample emulsion reaction generating device and method, a mixed solvent extraction mixed medicine method and an extraction liquid filtering method. In the solvent extraction process, an operation control method achieves the effect that medicine and whole blood are separated through solvents; by the extraction liquid filtering method, a filtering membrane is subjected to modification treatment; the continuous photoionization ion mobility spectrometry analysis is facilitated; the detection sensitivity is improved. The method has the advantage that a work foundation is laid for analyzing the blood concentration of medicine in the intraoperative whole blood sample by an ion mobility spectrometer.

Description

technical field [0001] The invention relates to a method for extracting drugs from intraoperative whole blood samples used for ion mobility spectrometry analysis. The method is simple, fast and has good reproducibility in preparing samples. The whole blood sample extraction method includes a whole blood sample emulsification reaction generation device and method, a mixed solvent extraction method and an extraction liquid filtration method. This method lays a working foundation for ion mobility spectrometer analysis of blood drug concentration in whole blood samples during operation. Background technique [0002] The whole blood after anticoagulation treatment is whole blood; the light yellow liquid obtained after centrifugation to remove blood cells is plasma. If the blood is allowed to coagulate without anticoagulation treatment, the blood will coagulate automatically under the action of a series of coagulation factors within a period of time after the blood is drawn. The...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N1/34
CPCG01N1/34
Inventor 李恩有李海洋王新
Owner 李恩有
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