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Method for identifying high-fructose-syrup adulterated honey

A technology of high fructose syrup and honey, applied in the field of detection, can solve the problems of high misjudgment rate and poor scientific method.

Active Publication Date: 2018-07-27
BEE RES INST CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, there have been articles and some laboratory methods that have attempted to identify multiple high fructose syrup at the same time, but because there is no clear target or reference material as support, the method is not scientific and the misjudgment rate is high

Method used

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  • Method for identifying high-fructose-syrup adulterated honey
  • Method for identifying high-fructose-syrup adulterated honey
  • Method for identifying high-fructose-syrup adulterated honey

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] 1. Common high-fructose syrup samples of experimental samples, including 11 samples of rice high-fructose syrup, coded DM1-DM11; 23 samples of corn high-fructose syrup, coded YM1-YM23; 9 samples of beet high-fructose syrup, coded TC1-TC9; 14 sucrose high fructose syrup, numbered ZT1-ZT14; 6 cassava high fructose syrup.

[0030] 2. Real honey samples for experiments: 32 pieces of rapeseed honey, numbered YC1-YC32; 33 pieces of acacia honey, numbered YH1-YH33; 15 pieces of jujube honey, numbered ZH1-ZH 15; 12 pieces of linden honey, numbered DS1-DS 12; Vitex honey 30, coded JT1-JT 30; lychee honey, coded LZ1-LZ 5; longan honey 3, coded LY1-LY 3; buckwheat honey 4, coded QM1-QM4; sunflower honey 5, No. XRK1-XRK5; 7 pieces of Manuka honey, numbered MNK1-MNK 7; 4 pieces of clover honey, numbered SYC1-SYC 3; 32 pieces of multifloral mixed honey, numbered DHZ1-DHZ 32; 13 pieces of medium honey, numbered ZF1 -ZF13.

[0031] 3. Sample extraction and derivatization (in the thre...

Embodiment 2

[0059] Sample source: Apiculture Central Station of Shandong Province, 20 suspected high fructose syrup adulterated samples were submitted for inspection, 15 of which were determined by the stable carbon isotope ratio method of GB / T18932.1-2002 Determination of Carbon-4 Plant Sugar Content in Honey, confirmed Adulterated corn high fructose syrup. Other suspected 2 samples were considered to be adulterated samples of beet high fructose syrup, 2 samples were adulterated samples of rice high fructose syrup, and 1 sample was adulterated sample of malt high fructose syrup.

[0060] Sample treatment: Weigh 2 g of the sample, add 2 mL of pH=6.0 phosphate buffer solution to dissolve, add 1 mL of 1% o-phenylenediamine solution, mix for 1 min, react overnight in the dark for 12 h, filter at 0.22 μm, and use ultra-high performance liquid chromatography Tandem mass spectrometry.

[0061] Liquid chromatography tandem mass spectrometry conditions: Agilent 6495 tandem mass spectrometer, col...

Embodiment 3

[0076] Honey sample is with embodiment 1. Step 3: Weigh 2 g of high fructose syrup or honey sample, dissolve in 2 mL of pH=5.0 phosphate buffer solution, add 1 mL of 1% 5,6-diamino-2,4-dihydroxypyrimidine aqueous solution, and react in the dark at 100°C After 1 h, the ice cubes were cooled.

[0077] Other operations are the same as in Example 1.

[0078] The derivative structures obtained when diffracting with different derivatizing agents are as follows:

[0079] The 5,6-diamino-2,4-dihydroxypyrimidine derivatives of 1,2-diketo-pentoses are:

[0080]

[0081] The 5,6-diamino-2,4-dihydroxypyrimidine derivatives of 2,3-diketo-pentose sugars are:

[0082]

[0083] The 5,6-diamino-2,4-dihydroxypyrimidine derivative of 3-deoxy-1,2-diketo-pentose is:

[0084]

[0085] The 5,6-diamino-2,4-dihydroxypyrimidine derivatives of 1-deoxy-2,3-diketo-pentose are:

[0086]

[0087] Derivatizing agent 5,6-diamino-2,4-dihydroxypyrimidine, the typical mass spectrum information (...

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Abstract

The invention provides a method for identifying high-fructose-syrup adulterated honey. The method takes an alpha-dicarbonyl compound as a target substance to detect a honey sample containing the target substance, i.e., the high-fructose-syrup adulterated honey. The alpha-dicarbonyl compound with an indication effect comprises one or more of 1,2-diketone-pentose, 2,3-diketone-pentose, 3-deoxy-1,2-diketone-pentose and 1-deoxy-2,3-diketone-pentose. According to the method provided by the invention, four detected and screened alpha-dicarbonyl compounds are used as a target compound for identifyingvarious types of high-fructose-syrup adulteration. The method is simple and effective and the main high-fructose-syrup adulterated honey at present can be covered through one time of analysis.

Description

technical field [0001] The invention belongs to the technical field of detection, and in particular relates to a detection method for honey mixed with high fructose syrup. Background technique [0002] Honey is a natural sweet substance that bees collect from the nectar, secretion or honeydew of plants, combine with their own secretions, and fully brew. Because honey has important nutritional value and health care function, it has been loved by people for a long time. Honey is an extremely easy food to be adulterated. Driven by profit, in recent years, some self-employed and manufacturers have added sugar, invert sugar, fructose, glucose, glucose high fructose syrup, fructose, etc. to honey to make adulterated honey. Alternative raw materials such as high fructose syrup and honey essence are used to manufacture honey, which is put on the market in the form of natural honey, defrauding consumers for huge profits, and damaging the reputation of natural honey in my country. E...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06G01N2030/042G01N2030/067
Inventor 薛晓锋孙明辉吴黎明王凯赵玲玲
Owner BEE RES INST CHINESE ACAD OF AGRI SCI
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