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A kind of polypeptide and its application

A polynucleotide and amino acid technology, applied in the field of polypeptides and their applications, can solve the problems of limited, unclear, and increased p53

Active Publication Date: 2021-04-02
RENJI HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, people's understanding of these proteins is still relatively limited, and it is not clear whether there are other proteins that play similar roles
It is precisely because of the above problems that there may be certain limitations in principle to increase the expression level of p53 simply by inhibiting MDM2, or to increase the acetylation of p53 by using SIRT1 inhibitors

Method used

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  • A kind of polypeptide and its application
  • A kind of polypeptide and its application
  • A kind of polypeptide and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] The basic procedure for the preparation of the polypeptide involved in the present invention is as follows: first, an amino acid protected by an Fmoc group is connected to an insoluble solid-phase carrier Wang resin, and then the protecting group of the amino group is removed, and the first amino acid is connected to the solid phase carrier Wang resin. Secondly, the carboxyl group of the second amino acid whose amino group is blocked is activated by a condensing agent, and the second amino acid whose carboxyl group is activated reacts with the amino group of the first amino acid that has been connected to the solid phase carrier to form a peptide bond, thereby A dipeptide with a protecting group is formed on the solid support. Repeat the above peptide bond formation reaction to make the peptide chain grow from the C-terminus to the N-terminus until the desired length of the peptide chain is reached. Finally, the ester bond between the peptide chain and the solid phase c...

Embodiment 2

[0039] In vitro culture of tumor cells: Human colorectal cancer tumor cells HCT116 (expressing wild-type p53, purchased from ATCC) or other cells were cultured in vitro according to conventional methods, using DMEM or RPMI1640 medium containing 10-20% calf serum, Cultivate in an incubator containing 5% carbon dioxide at 37 degrees Celsius, and subculture when the cell density reaches 80%-90%.

[0040] The administration method of Morn3(222-237) polypeptide: (1) Culture the above-mentioned cells with 6-well plates, when the cell density reaches 50-60%, add Morn3(222-237) polypeptide to the culture medium, until the final The concentration is 1 μM to 2.5 μM or other concentrations, and continue to incubate in the incubator for more than 4 hours.

[0041] Western Blot experiment was used to detect the effect of Morn3 (222-237) polypeptide on p53 protein expression and acetylation level: add 0.1 ml of RIPA lysate (containing protease inhibitors) to each well of the 6-well plate co...

Embodiment 3

[0043] RT-qPCR experiment to detect the effect of Morn3(222-237) polypeptide on the transcriptional activation of p53 downstream genes: Morn3(222-237) polypeptide (or PBS, control polypeptide) was added to HCT116 cells to a final concentration of 2.5 μM, and a total of After culturing for 48 hours, total RNA was extracted, and cDNA was obtained by reverse transcription with random primers. Use p21, BAX, PUMA and other p53 downstream gene-specific primers for qPCR amplification to detect changes in the transcriptional activation ability of p53 (see: Xu J, et al., CellRep.2013; 3(5):1526-38). After Morn3(222-237) polypeptide was co-cultured with cells, the transcriptional activation of p21, BAX, and PUMA was significantly increased, as shown in Figure 4 shown. Figure 4 The vertical axis in is the ratio of other treatment groups with PBS as 1.0. Figure 4-6 The asterisks in * represent statistical test P<0.01 (two-sided T test), and there are significant differences between t...

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Abstract

The invention relates to a polypeptide. The polypeptide has a function of adjusting a cancer suppressor protein p53 and has an amino acid sequence shown in the formula of SEQ ID NO: 1 or an amino acidsequence similar to the amino acid sequence shown in the formula of SEQ ID NO: 1. The polypeptide has functions of positively regulating p53 protein expression and positively regulating the acetylation of the p53 protein. The polypeptide provides a basis for preparation and screening of an apoptosis-inducing agent and an anti-tumor drug based on the p53 protein expression and acetylation regulation mechanism. The invention also relates to a use of the polypeptide.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a polypeptide and its application. Background technique [0002] The occurrence of malignant tumors is related to the inactivation of tumor suppressor protein p53, and reactivation of p53 in tumors is a potential strategy for tumor-targeted therapy. The cancer-associated protein p53 was found to be highly expressed in tumor cells in 1979, and was initially thought to be a cancer-promoting protein. However, later researchers found that mutant p53 was overexpressed in tumors, while wild-type p53 transcriptionally activated p21, Bax, Puma and other genes under the condition of DNA damage and oncogene activation, inhibited cell proliferation and induced apoptosis, thereby exerting The role of tumor suppressor. In tumors, inactivation of wild-type p53 is associated with its ubiquitination and deacetylation. British scholar K. Vousden found that the MDM2 gene promotes the ub...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/47C12N15/12C12N15/63A61K38/17A61P35/00
CPCA61K38/00C07K14/4746
Inventor 许杰王焕彬梁纶熙姚晗章瑶李楚舒石虎兵徐琪陈萦晅房静远
Owner RENJI HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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