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Weed suppressing fungus screened from seaweeds and extract and application thereof

A technology of herb-inhibiting fungi and extracts, applied in the fields of application, fungi, microorganisms, etc., can solve the problem of no secondary metabolites to prevent and control weeds, report, etc., achieve excellent herbicidal performance, high preparation efficiency, and good effect

Active Publication Date: 2018-07-31
ZUNYI TOBACCO OF GUIZHOU TOBACCO CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is still little research on the application of fungal herbicides in seaweeds, and there is no report on the control of weeds by the secondary metabolites of fungi in seaweeds

Method used

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  • Weed suppressing fungus screened from seaweeds and extract and application thereof
  • Weed suppressing fungus screened from seaweeds and extract and application thereof
  • Weed suppressing fungus screened from seaweeds and extract and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Embodiment 1 screens the herbaceous fungus Aspergillus versicolor D5 from seaweed

[0035] Rinse the seaweed with tap water, weigh 5g, soak in 75% alcohol for 1min under sterile conditions, rinse with sterile water, cut the sample into small pieces, grind the sample with a sterile mortar, add appropriate amount of sterile water Afterwards, 100 microliters were drawn, spread evenly on the PDA medium with a coating rod, and cultivated in a 28° C. incubator for 72 hours.

[0036] Name different strains according to the characteristics of colony color, mycelium length, etc., and re-inoculate them on a new PDA plate for purification. The purified strains are genetically identified, and the weed inhibition of the strains is tested during seed germination, rhizome growth inhibition, and stem and leaf damage. activity, screen out the herbaceous fungus Aspergillus versicolor D5.

Embodiment 2

[0037] The cultivation and fermentation of embodiment 2 herbaceous fungus Aspergillus versicolor D5

[0038] (1) Potato dextrose agar medium is used for the strain culture of the herbaceous fungus Aspergillus versicolor D5, containing 5-6g / L of potato, 15-20g / L of glucose, 18-20g / L of agar, and 25-30g / L of coarse sea salt, The rest is water, which is made into plate medium when used, and the strains are cultivated at 28°C for 7-10 days;

[0039] (2) The fermentation culture of the herbaceous fungus Aspergillus versicolor D5 adopts potato glucose liquid medium, and in a 1000mL Erlenmeyer flask, add potato extract powder 5-6g / L, glucose 15-20g / L, coarse sea salt 25-30g / L, water 350-400mL, inoculated with fungi, fermented at 28°C for 30-40 days.

[0040] The obtained fermentation broth was extracted with ethyl acetate and concentrated; the fermented cells were soaked in a solution of methanol:dichloromethane at a ratio of 1:1, concentrated under reduced pressure, and the remaini...

Embodiment 3

[0041] The herbicidal activity test of embodiment 3 herbicidal fungus extract

[0042] Seed Germination Method:

[0043] Sterilize Amaranthus reflexae seeds with 3%-5% sodium hypochlorite (NaClO) solution for 5-15min, rinse several times with sterile water to remove sodium hypochlorite. Experiments were performed using 24-well plates.

[0044] The herbastatic fungus extract of Example 2 was dissolved in 100% methanol to prepare a 1.0 mg / mL solution.

[0045] Put filter paper in a 24-well plate, add 200 μL or 400 μL extract solution to each well, after the solvent evaporates, add 200 μL or 400 μL sterile water to each well, seal and place in a light incubator at 28°C, 12h light, 12h dark culture . Count germination rate and root (bud) inhibition rate after 4 days, see Table 1. Glyphosate was used as a positive control.

[0046] Root (bud) inhibition rate (%) = [(control group root (bud) length - treatment group root (bud) length) ÷ control group root (bud) length] × 100

...

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Abstract

The invention provides a weed suppressing fungus screened from seaweeds and an extract and application thereof and belongs to the field of fungal extracts. The weed suppressing fungus screened from the seaweeds has weeding activity, can be applied to plowland as a highly-potent herbicide and particularly has high weeding activity to seeds, stems and leaves of dicotyledonous weeds represented by Amaranthus retroflexus. The weed suppressing fungus screened from the seaweeds is Aspergillus versicolor D5, is collected in CGMCC and has a collection number of CGMCC 15386.

Description

technical field [0001] The invention relates to the field of fungal extracts, in particular to a herbaceous fungus screened from seaweed, its extract and application. Background technique [0002] At present, there are more than 30,000 species of weeds widely distributed all over the world, of which more than 1,800 species are harmful to the growth of crops. If these weeds are not controlled, the world's grain production can be reduced by 10%-15%, and in severe cases, the production can even be reduced by more than 50%. Weeds not only compete with crops for the nutrients needed for normal growth, but also many weeds are intermediate hosts for the spread of diseases and insect pests. Most of the perennial and perennial weeds are overwintering places for overwintering diseases and insect pests, which greatly affects the yield of crops and quality. [0003] In the past half century, chemical herbicides have developed rapidly due to their rapid action and convenient use. They ...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12P1/02A01N63/04A01P13/02C12R1/66
CPCA01N63/30C12P1/02C12N1/145C12R2001/66
Inventor 韩小斌芶剑渝赵建赵栋霖刘明宏张成省彭玉龙李义强刘京张之矾马国勇祝乾湘
Owner ZUNYI TOBACCO OF GUIZHOU TOBACCO CORP
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