SERS substrate preparation and detection integrated method based on micro flow mixer
A mixer and substrate technology, used in instruments, measuring devices, scientific instruments, etc., can solve the problem of low utilization rate of "hot spots", and achieve the effect of maintaining natural activity, enhancing effect, and reducing oxidation effect.
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[0030] Example 1: Homogeneity characterization of microfluidic mixer-based SERS substrates
[0031] The silver nanoparticle colloidal solution was introduced simultaneously from the two side channels of the freshly prepared C-SAR microfluidic mixer at a flow rate of 200 μl / min; 2.2 nM R6G solution was introduced into the main channel at a flow rate of 40 μl / min; The mixing zone mixes the analyte and metal nanoparticles sufficiently and rapidly; R6G can be uniformly adsorbed on the surface of the nanoparticles. R6G-adsorbed silver nanoparticles were deposited in the detection area of the microfluidic chip. After 10 minutes, deionized water was passed through the three inlet channels to remove excess unadsorbed particles, and a uniform single-layer silver nanoparticle film was obtained. like figure 2 shown. Randomly detect 60 points in the detection area, and the obtained SERS spectrum of R6G is as follows image 3 shown, the deviation in signal strength is only 11%.
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[0032] Example 2: Long-term stability characterization of microfluidic mixer-based SERS substrates
[0033] The silver nanoparticle colloidal solution was introduced simultaneously from the two side channels of the freshly prepared C-SAR microfluidic mixer at a flow rate of 200 μl / min; 2.2 nM R6G solution was introduced into the main channel at a flow rate of 40 μl / min; The mixing zone mixes the analyte and metal nanoparticles sufficiently and rapidly; R6G can be uniformly adsorbed on the surface of the nanoparticles. The R6G-adsorbed silver nanoparticles were deposited in the detection area of the microfluidic chip. After 10 minutes, deionized water was passed through the three inlet channels to remove the excess unadsorbed particles, and a uniform single-layer silver nanoparticle film was obtained, which was obtained in the detection area. The SERS spectrum of R6G, the chip was placed in the air for 10 weeks, and the SERS spectrum of R6G in the detection area was tested on...
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[0034] Example 3: Characterization of Detection Capability of Microfluidic Mixer-Based SERS Substrates
[0035] The silver nanoparticle colloidal solution was simultaneously introduced from the two side channels of the freshly prepared C-SAR microfluidic mixer at a flow rate of 200 μl / min; 10 -7 ,5×10 -8 ,10 -8 ,5×10 -9 ,10 -9 The R6G solution of M was respectively passed through the main channel at a flow rate of 40 μl / min; the analyte and the metal nanoparticles were sufficiently and rapidly mixed in the mixing area; R6G could be uniformly adsorbed on the surface of the nanoparticles. The concentration of R6G in the detection area was 9 × 10 -9 ,4.5×10 -9 ,9×10 -10 ,4.5×10 -10 ,2×10 -10 and 9×10 -11 M, R6G-adsorbed silver nanoparticles were deposited in the detection area of the microfluidic chip. After 10 minutes, deionized water was passed through the three inlet channels to remove excess unadsorbed particles, and a series of uniform single-layer silver nanopart...
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