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SERS substrate preparation and detection integrated method based on micro flow mixer

A mixer and substrate technology, used in instruments, measuring devices, scientific instruments, etc., can solve the problem of low utilization rate of "hot spots", and achieve the effect of maintaining natural activity, enhancing effect, and reducing oxidation effect.

Active Publication Date: 2018-07-31
SOUTHEAST UNIV
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Problems solved by technology

[0005] Technical issues: Based on the problem of low utilization of "hot spots" in traditional solid-phase SERS substrates, an integrated method for SERS substrate preparation and detection based on microfluidic mixers was proposed to achieve high sensitivity, high uniformity, good repeatability and long-term Stable SERS detection and in situ research

Method used

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  • SERS substrate preparation and detection integrated method based on micro flow mixer
  • SERS substrate preparation and detection integrated method based on micro flow mixer
  • SERS substrate preparation and detection integrated method based on micro flow mixer

Examples

Experimental program
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Effect test

Embodiment 1

[0030] Example 1: Uniformity Characterization of SERS Substrate Based on Microfluidic Mixer

[0031] The colloidal solution of silver nanoparticles was introduced simultaneously from the two side channels of the freshly prepared C-SAR microfluidic mixer at a flow rate of 200 μl / min; the R6G solution of 2.2 nM was introduced from the main channel at a flow rate of 40 μl / min; The mixing zone fully and quickly mixes the analyte and metal nanoparticles; R6G can be evenly adsorbed on the surface of the nanoparticles. The silver nanoparticles adsorbed with R6G were deposited on the detection area of ​​the microfluidic chip. After 10 minutes, deionized water was passed through the three inlet channels to remove excess unadsorbed particles, and a uniform single-layer silver nanoparticle film was obtained. Such as figure 2 shown. 60 points are randomly detected in the detection area, and the obtained SERS spectrum of R6G is as follows image 3 As shown, the deviation of the signal ...

Embodiment 2

[0032] Example 2: Characterization of long-term stability of SERS substrates based on microfluidic mixers

[0033] The colloidal solution of silver nanoparticles was introduced simultaneously from the two side channels of the freshly prepared C-SAR microfluidic mixer at a flow rate of 200 μl / min; the R6G solution of 2.2 nM was introduced from the main channel at a flow rate of 40 μl / min; The mixing zone fully and quickly mixes the analyte and metal nanoparticles; R6G can be evenly adsorbed on the surface of the nanoparticles. The silver nanoparticles adsorbed with R6G were deposited on the detection area of ​​the microfluidic chip. After 10 minutes, the three inlet channels were fed with deionized water to remove excess unadsorbed particles, and a uniform single-layer silver nanoparticle film was obtained. For the SERS spectrum of R6G, place the chip in the air for 10 weeks, and test the SERS spectrum of R6G in the detection area once a week, such as Figure 4 As shown, there...

Embodiment 3

[0034] Example 3: Characterization of the detection capability of the SERS substrate based on the microfluidic mixer

[0035] The colloidal solution of silver nanoparticles was simultaneously passed through two side channels of the freshly prepared C-SAR microfluidic mixer at a flow rate of 200 μl / min; 10 -7 ,5×10 -8 ,10 -8 ,5×10 -9 ,10 -9 The R6G solution of M was passed through the main channel at a flow rate of 40 μl / min; the analyte and metal nanoparticles were mixed fully and rapidly in the mixing zone; R6G could be evenly adsorbed on the surface of the nanoparticles. The concentration of R6G in the detection area is 9×10 -9 ,4.5×10 -9 ,9×10 -10 ,4.5×10 -10 ,2×10 -10 and 9×10 -11 M, silver nanoparticles adsorbed with R6G were deposited on the detection area of ​​the microfluidic chip, and after 10 minutes, the three inlet channels were fed with deionized water to remove excess unadsorbed particles, and a series of uniform single-layer silver nanoparticle films we...

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Abstract

The invention discloses a surface enhanced Raman scattering (SERS) substrate preparation and detection integrated method based on a micro flow mixer. The SERS substrate preparation and detection integrated method comprises following steps: the micro flow mixer is adopted for uniform and rapid mixing of analytes and metal nanometer particles; the analytes are absorbed by metal nanometer particle surfaces uniformly; compact deposition of the metal nanometer particles with absorbed analytes onto a detection zone of the micro flow mixer is realized so as to form a high sensitivity solid phase SERSsubstrate; SERS detection is carried out; and the micro flow mixer is used for flexible changing of micro environment in a channel, and in-suit SERS research on the analytes is carried out. Accordingto the SERS substrate preparation and detection integrated method, integration of all the functions onto the 1.25cm*1.85cm micro flow mixer is realized, experiment time is shortened greatly, errors caused by each step in separated experiments are reduced, detection reliability and long term stability of detection are increased. The SERS substrate prepared in the SERS substrate preparation and detection integrated method is capable of realizing coupling of the analytes to a hot spot area, so that extremely high SERS enhancing effect is achieved; and in addition, uniform and rapid mixing is capable of realizing analyte uniform absorption, so that uniform SERS signals are achieved.

Description

technical field [0001] The invention relates to a high-efficiency detection technology, which belongs to the technical field of functionalized microfluidic devices, and in particular proposes a method for preparing a SERS substrate that utilizes an ultrafast microfluidic mixer to achieve a high utilization rate of "hot spots". Background technique [0002] Surface-enhanced Raman scattering (SERS) detection technology has been applied in various fields, including environmental monitoring, food safety, homeland security, biomedicine, etc. The characteristics of its fingerprint spectrum are conducive to a better understanding of the structure of biological macromolecules and the study of the change process of biological macromolecules. Microfluidic devices can observe the process of biological and chemical reactions in real time in a flowing state. Therefore, SERS functionalized microfluidic devices (LoC-SERS) are of great significance in current research applications. [0003...

Claims

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Application Information

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IPC IPC(8): G01N21/01G01N21/65
CPCG01N21/01G01N21/658
Inventor 朱利陆辉张若虎崔一平
Owner SOUTHEAST UNIV
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