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A glycoprotein surface imprinted polymer based on team boron affinity and its preparation method and application

A technology of surface imprinting and polymers, applied in chemical instruments and methods, and other chemical processes, can solve the problems of poor adsorption and separation, easy loss of biological activity, and low selectivity, so as to maintain biological activity and avoid secondary pollution , the effect of expanding the scope of application

Active Publication Date: 2020-02-21
广州金葵生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to overcome the disadvantages of poor adsorption and separation of glycoproteins, low selectivity, and easy loss of biological activity of imprinted adsorbents in the prior art under physiological conditions, and provide a glycoprotein surface based on team boron affinity Preparation method of imprinted polymer

Method used

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  • A glycoprotein surface imprinted polymer based on team boron affinity and its preparation method and application
  • A glycoprotein surface imprinted polymer based on team boron affinity and its preparation method and application
  • A glycoprotein surface imprinted polymer based on team boron affinity and its preparation method and application

Examples

Experimental program
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Effect test

Embodiment 1

[0039] (1) Preparation of TBA-modified Fe3O4 nanoparticles:

[0040] First, for Fe 3 o 4 @PGMA nanoparticles for surface modification. First, 0.1 g of 3-aminophenylboronic acid and 0.1 g of 1,6-hexanediamine were dispersed in 20 mL of ethanol, followed by stirring at 30 °C for 30 min to form B-N coordinated team boron affinity molecules. Then add 0.025 g core-shell Fe 3 o 4 @PGMA nanoparticles and reflux at 60°C for 12h, and then dry to constant weight in a vacuum oven at 40°C to obtain TBA-modified ferric oxide nanoparticles (Fe 3 o 4 @PGMA-TBA).

[0041] (2) Preparation of imprinted polymers on the surface of glycoproteins:

[0042] First, 0.005 g template protein egg albumin and 0.025 g Fe obtained in step (1) 3 o 4 @PGMA-TBA was added to 20 mL of phosphate buffer solution with pH=7.4, ultrasonically dispersed for 5 min, and then left to stand in the dark for 0.5 h to allow egg albumin and Fe 3 o 4 The TBA molecules on the @PGMA-TBA surface are bound together by ...

Embodiment 2

[0048] (1) Preparation of TBA-modified Fe3O4 nanoparticles:

[0049] First, for Fe 3 o 4 @PGMA nanoparticles for surface modification. First, 0.167 g of 3-aminophenylboronic acid and 0.138 g of 1,6-hexanediamine were dispersed in 40 mL of ethanol, followed by stirring at 40 °C for 60 min to form B-N coordinated team boron affinity molecules. Then add 0.050 g core-shell Fe 3 o 4 @PGMA nanoparticles and reflux at 60-100°C for 12h, and then dry to constant weight in a vacuum oven at 40-60°C to obtain TBA-modified ferric oxide nanoparticles (Fe3O4@PGMA-TBA).

[0050] (2) Preparation of imprinted polymers on the surface of glycoproteins:

[0051] First, 0.010 g template protein egg albumin and 0.050 g Fe obtained in step (1) 3 o 4 @PGMA-TBA was added to 30 mL of phosphate buffer solution with pH=7.4, ultrasonically dispersed for 10 min, and then left to stand in the dark for 1 h to allow egg albumin to bind to the TBA molecules on the surface of Fe3O4@PGMA-TBA through boron ...

Embodiment 3

[0053] (1) Preparation of TBA-modified Fe3O4 nanoparticles:

[0054] First, for Fe 3 o 4 @PGMA nanoparticles for surface modification. First, 0.2 g of 3-aminophenylboronic acid and 0.15 g of 1,6-hexanediamine were dispersed in 60 mL of ethanol, followed by stirring at 60 °C for 390 min to form B-N coordinated team boron affinity molecules. Then add 0.075 g core-shell Fe 3 o 4 @PGMA nanoparticles and refluxed at 100 °C for 12 h, and then dried to constant weight in a vacuum oven at 60 °C to obtain TBA-modified ferric oxide nanoparticles (Fe3O4@PGMA-TBA).

[0055] (2) Preparation of imprinted polymers on the surface of glycoproteins:

[0056] First, 0.015 g template protein egg albumin and 0.075 g Fe obtained in step (1) 3 o 4 @PGMA-TBA was added to 40 mL of pH=7.4 phosphate buffer solution, ultrasonically dispersed for 15 min, and then left to stand in the dark for 1.5 h to allow egg albumin and Fe 3 o 4 The TBA molecules on the @PGMA-TBA surface are bound together by ...

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Abstract

The invention relates to a glycoprotein surface imprinted polymer based on team boron affinity as well as a preparation method and an application of the glycoprotein surface imprinted polymer, and belongs to the technical field of preparation of medical functional materials. The method comprises the following steps: firstly, modifying Fe3O4@PGMA nanoparticles of a core-shell structure with a teamboron affinity molecule to functionalize surface boric acid; secondly, co-culturing the modified Fe3O4@PGMA with template glycoprotein, immobilizing the template protein on the surface of the particles, performing a series of treatment after aniline polymerization initiated by oxidation to obtain an adsorbent, and adopting the adsorbent for selective recognition and separation of glycoprotein under the neutral condition. The prepared glycoprotein surface imprinted polymer based on team boron affinity is easy to prepare, can effectively avoid secondary pollution caused by pH adjustment, has good reusability and higher adsorption capacity to glycoprotein under the neutral condition, has obvious glycoprotein recognition performance, and can well maintain biological activity of glycoprotein.

Description

technical field [0001] The invention relates to a preparation method of an imprinted polymer, in particular to a glycoprotein surface imprinted polymer based on team boron affinity and its preparation method and application, belonging to the technical field of preparation of medical functional materials. Background technique [0002] Glycoproteins are widely distributed in various tissues and cells, and have important biological functions, such as participating in cell adhesion and signal transduction, immune and inflammatory responses, and sperm-egg recognition. It is of great significance to isolate and detect glycoproteins, especially glycoproteins that can be used as cancer markers. Therefore, there is a need for a method that can efficiently and selectively isolate glycoproteins while maintaining their biological activity. [0003] Adsorption is an effective method for separating glycoproteins from complex biological samples, however, the selectivity needs to be improv...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01J20/26B01J20/30
CPCB01J20/268
Inventor 朱恒佳潘建明刘金鑫夏可旭
Owner 广州金葵生物科技有限公司
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