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Method for simultaneously measuring four natural products in health care wine by UPLC-Q-Tof/MS

A natural product, uplc-q-tof technology, applied in the field of detection, can solve the problems of complex steps, inability to meet rapid analysis and detection, and time-consuming

Active Publication Date: 2018-08-03
ANHUI RUISIWEIER TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method takes a long time and has complicated steps, which cannot meet the requirements of rapid analysis and detection

Method used

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  • Method for simultaneously measuring four natural products in health care wine by UPLC-Q-Tof/MS
  • Method for simultaneously measuring four natural products in health care wine by UPLC-Q-Tof/MS
  • Method for simultaneously measuring four natural products in health care wine by UPLC-Q-Tof/MS

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Embodiment Construction

[0034] In order to better understand the present invention, the content of the present invention will be further clarified below in conjunction with specific examples, but the content of the present invention is not limited to the following examples, and the examples should not be regarded as limiting the scope of protection of the present invention.

[0035] 1 Instruments and samples

[0036] 1.1 Instrument:

[0037] Ultra performance liquid chromatography system (ACQUITY Ultra Performance LC Waters company) equipped with mass spectrometry (G2-Q Tof time-of-flight mass spectrometer Waters company), rotary evaporator (Switzerland Buchi R-210), ACQUITY UPLC BenC18Column (1.7μm, 2.1 ×50mm) chromatographic column.

[0038] 1.2 Sample: Black wolfberry health wine

[0039] 2 methods and results

[0040] 2.1.1 Preparation of standard products: dissolve betaine, rutin, resveratrol, and folic acid in acetonitrile to prepare mother liquor standards. The concentrations of mother liquor standards ...

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Abstract

The invention discloses a method for simultaneously measuring four natural products in health care wine by a UPLC-Q-Tof / MS. Firstly, the C18 chromatographic column of Ben is used for carrying out gradient elution separation, then, an electrospray ionization source is adopted to scan under a positive ion mode, feature ions corresponding to the four natural products are extracted, an integral peak area is obtained, and therefore, glycine betaine, rutin, resveratrol and folic acid are quickly analyzed and authenticated, By use of the method, the glycine betaine, the rutin, the resveratrol and thefolic acid in the hear care wine are quickly, accurately and simultaneously detected, and the ion features of the glycine betaine, rutin, resveratrol and folic acid in the mass spectrum can be givenso as to realize the accurate qualitative and quantitative analysis of the glycine betaine, the rutin, the resveratrol and the folic acid in health care wine.

Description

Technical field [0001] The invention relates to a UPLC-Q-Tof / MS method for simultaneously determining four natural products in health wine, belonging to the technical field of detection. Background technique [0002] Studies have proved that betaine has certain therapeutic effects on human cardiovascular, neurological, liver and homocysteinuria diseases; resveratrol has anti-tumor, lowering blood pressure, anti-platelet aggregation, anti-bacteria and fungus Infection, affecting gastric acid secretion and many other physiological activities; as a typical representative of flavonoids, rutin has anti-free radical activity, anti-lipid peroxidation, anti-virus, antagonistic effect of platelet activating factor, anti-acute pancreatitis, and vasodilation As well as the protection of gastric ulcer and other physiological activities or pharmacological effects; as an indispensable nutrient for fetal growth and development, folic acid participates in the metabolism of genetic material and p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/06G01N30/88
CPCG01N30/06G01N30/88
Inventor 马金同刘露马雷胡心行刘国英李安军周庆伍沈小梅
Owner ANHUI RUISIWEIER TECH
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