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A method for the simultaneous determination of four natural products in health wine by uplc-q-tof/ms

A natural product, uplc-q-tof technology, applied in the field of detection, can solve the problems of inability to meet rapid analysis and detection, time-consuming, complicated steps, etc.

Active Publication Date: 2020-05-01
ANHUI RUISIWEIER TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

This method takes a long time and has complicated steps, which cannot meet the requirements of rapid analysis and detection

Method used

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  • A method for the simultaneous determination of four natural products in health wine by uplc-q-tof/ms
  • A method for the simultaneous determination of four natural products in health wine by uplc-q-tof/ms
  • A method for the simultaneous determination of four natural products in health wine by uplc-q-tof/ms

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Embodiment Construction

[0034] In order to better understand the present invention, the content of the present invention is further illustrated below in conjunction with specific examples, but the content of the present invention is not limited to the following examples, and the examples should not be regarded as limiting the protection scope of the present invention.

[0035] 1 Instruments and samples

[0036] 1.1 Instruments:

[0037] Ultra-high performance liquid chromatography system (ACQUITY Ultra Performance LC Waters Company) equipped with mass spectrometer (G2-Q Tof time-of-flight mass spectrometer Waters Company), rotary evaporator (Swiss Buchi R-210), ACQUITY UPLC BenC18Column (1.7μm, 2.1 ×50mm) chromatographic column.

[0038] 1.2 Sample: black wolfberry health wine

[0039] 2 Methods and Results

[0040] 2.1.1 Preparation of standard products: Dissolve betaine, rutin, resveratrol, and folic acid in acetonitrile respectively to prepare mother liquor standards. L, resveratrol 400mg / L, f...

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Abstract

The invention discloses a method for simultaneously measuring four natural products in health care wine by a UPLC-Q-Tof / MS. Firstly, the C18 chromatographic column of Ben is used for carrying out gradient elution separation, then, an electrospray ionization source is adopted to scan under a positive ion mode, feature ions corresponding to the four natural products are extracted, an integral peak area is obtained, and therefore, glycine betaine, rutin, resveratrol and folic acid are quickly analyzed and authenticated, By use of the method, the glycine betaine, the rutin, the resveratrol and thefolic acid in the hear care wine are quickly, accurately and simultaneously detected, and the ion features of the glycine betaine, rutin, resveratrol and folic acid in the mass spectrum can be givenso as to realize the accurate qualitative and quantitative analysis of the glycine betaine, the rutin, the resveratrol and the folic acid in health care wine.

Description

technical field [0001] The invention relates to a UPLC-Q-Tof / MS method for simultaneously measuring four natural products in health-care wine, which belongs to the technical field of detection. Background technique [0002] Studies have proved that betaine has a certain therapeutic effect on human cardiovascular, nervous, liver and homocystinuria and other diseases; resveratrol has anti-tumor, blood pressure lowering, anti-platelet aggregation, anti-bacteria and fungi Infection, affecting gastric acid secretion and other physiological activities; Rutin, as a typical representative of flavonoids, has anti-free radical activity, anti-lipid peroxidation, anti-virus, antagonism of platelet activating factor, anti-acute pancreatitis, and vasodilation And the protective effect on gastric ulcer and other physiological activities or pharmacological effects; as an indispensable nutrient for fetal growth and development, folic acid has physiological functions such as participating in ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/06G01N30/88
CPCG01N30/06G01N30/88
Inventor 马金同刘露马雷胡心行刘国英李安军周庆伍沈小梅
Owner ANHUI RUISIWEIER TECH
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