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Virus standard substance for assessment of protective performance of personnel protective equipment and preparation method of virus standard substance

A standard substance and protective performance technology, applied in the field of virus standard substance and its preparation for the evaluation of the protective performance of personal protective equipment, can solve the problems of detection error, labor, material and time consumption, trivial process of configuring indicator virus suspension, etc., and achieve The effect of reducing mortality, easy to use, and easy to transport

Active Publication Date: 2018-08-07
NAT INST OF METROLOGY CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, since there is no virus standard substance for evaluation at present, in the actual field experiment of the protective performance test of personal protective equipment, the inspectors often can only configure the indicator virus suspension according to their work experience, and it is difficult to ensure that the values ​​are completely consistent, resulting in detection errors
In addition, the process of preparing the indicator virus suspension is relatively trivial, which consumes a lot of manpower, material resources and time, which greatly affects the accuracy of the evaluation results and the efficiency of the evaluation work.

Method used

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  • Virus standard substance for assessment of protective performance of personnel protective equipment and preparation method of virus standard substance
  • Virus standard substance for assessment of protective performance of personnel protective equipment and preparation method of virus standard substance
  • Virus standard substance for assessment of protective performance of personnel protective equipment and preparation method of virus standard substance

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Example 1 Preparation of virus standard substance (bacteriophage ΦX174 standard substance) for evaluation of protective performance of personal protective equipment

[0042] 1. Materials, instruments and methods

[0043] 1. Test material

[0044] Phage ΦX174 (ATCC 13706B1) and host Escherichia coli 13706 (ATCC 13706) (bacteria preservation bank of Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences); nutrient agar medium, nutrient broth medium, phosphate buffered saline (Phosphate Buffered Saline, PBS), sterile saline (0.85%) (Beijing Land Bridge Technology Co., Ltd.); trehalose, soluble starch, sodium glutamate, sucrose, glucose, sodium ascorbate, BSA (Sigma, USA); brown vials (Shenzhen Baoan District Shajing Bohai Glass Products Factory).

[0045] 2. Instruments and equipment

[0046] Beta1-8LD plus freeze dryer (Christ, Germany); CR21G high-speed refrigerated centrifuge (Hitachi, Japan); HVE-50 autoclave (Hirayama, Japan); HWS-400 cons...

Embodiment 2

[0086] The homogeneity inspection of embodiment 2 standard substance

[0087] 1. Experimental method

[0088] 1. Homogeneity test method

[0089] According to the principle of unit sample extraction, the number of selected units and the number of repeated measurements for each sample should be suitable for the statistical test requirements adopted. When the total number of units is N1000, press to calculate the number of samples taken. For samples with good uniformity, when N500, the number of extraction units can be no less than 15. Therefore, 15 bottles were randomly selected from 200 bottles of bacteriophage ΦX174 standard substance samples, and after adding 1 mL of phosphate buffered saline to rehydrate in each bottle of samples, the plaque counting method was used to check according to the operation in step 9 in Example 1. Each bottle of sample was tested 3 times. Finally, the calculation results are tested for uniformity by the F test method.

[0090] 2. Uniformit...

Embodiment 3

[0120] The stability investigation of embodiment 3 standard substance

[0121] 1. Experimental method

[0122] 1. Short-term stability inspection method

[0123] The short-term stability investigation mainly involves the stability of the standard substance during transportation, so a statistical evaluation of the short-term stability of the standard substance at different temperatures is carried out. For the short-term stability study, different temperatures (-20°C, 4°C, 25°C, and 37°C) and different time points (0, 7, 14, 21, and 28 days) were used to test the plaque counting method. Select 3 bottles of samples, repeat the measurement 3 times for each bottle (N=3, n=3), and take the average value to obtain the experimental data of the short-term stability investigation of the virus standard substance used for the evaluation of the protective performance of personal protective equipment. According to the change of the live phage concentration of the virus standard substance ...

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Abstract

The invention provides a virus standard substance for assessment of protective performance of personnel protective equipment and a preparation method of the virus standard substance. The standard substance is composed of a lyophilized substance and a stabilizer aqueous solution, wherein the lyophilized substance contains phage phiX174 and a protective agent, and the stabilizer aqueous solution isused for rehydration of the phage lyophilized substance. According to experiments, the standard substance is high in uniformity and stability and convenient to use in assessment of the protective performance of the personnel protective equipment, manpower and time can be saved, and efficiency in assessment of the protective performance of the personnel protective equipment is greatly improved. Theinvention further provides a certificating method of the standard substance, phiX174 viable bacteria concentration accuracy can be guaranteed, and accuracy in assessment of the protective performanceof the personnel protective equipment is improved.

Description

Technical field: [0001] The invention relates to a virus standard substance for evaluating the protective performance of personnel protective equipment and a preparation method thereof, belonging to the technical field of biosafety. Background technique: [0002] In the evaluation of the protective performance of personnel protective equipment and facilities, the evaluation of the protective performance of microorganisms (such as viruses, bacteria, etc.) is the most critical. [0003] Phage ΦX174 is an aerobiological model virus with a particle size of 27-30nm. Because phage has the advantages of relatively simple cultivation and counting methods, high experimental safety, and obvious counting effect. Moreover, bacteriophage ΦX174 has the commonality of viruses and is a virus that uses viruses as hosts, so it is suitable as an indicator virus for biological detection and used in the evaluation of the protective performance of personal protective equipment such as biological...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/70C12N7/00C12R1/92
CPCC12N7/00C12Q1/06G01N2333/01C12N2795/14251
Inventor 隋志伟王晶刘思章
Owner NAT INST OF METROLOGY CHINA