Biological aerosol real-time detection method and device based on laser induced fluorescence

Abstract

The invention discloses a biological aerosol real-time detection method and device based on laser induced fluorescence. The primary parts of the device comprise a light path, a gas circuit intersectedwith the light path, and a signal processing system connected to the light path. The light path comprises a laser emitting light path, a scattered light receiving light path and a fluorescence receiving light path; the gas circuit is used for sampling to-be-detected particles; the signal processing system is used for analyzing and processing signals and comprises a scattered light processing system and a fluorescence processing system. Based on the principle of laser induced fluorescence, grain sizes and concentration of microbial particles and non-biological particles in air can be monitoredin real time. The grain size and the biological attribute of the to-be-detected particle are judged through the scattered light and fluorescence signals generated by the single particle under irradiation of laser, and the particles in a sampled air flow are counted, so that the concentration of microorganisms in air is detected automatically. The biological aerosol real-time detection method anddevice are simple to operate, high in real-time monitoring, high in sensitivity and high in accuracy.

Description

technical field [0001] The invention belongs to the field of air microorganism and particle monitoring, and in particular relates to a bioaerosol real-time monitoring method and device thereof, which can monitor the concentration, quantity, particle size, etc. of microbial particles such as bacteria and fungi in the air. Background technique [0002] The microorganisms that can survive in the air mainly include: (1) bacteria and viruses. The main indoor bacteria and viruses are hemolytic streptococcus, viridans streptococcus, pneumococcus, influenza virus, meningococcus, Mycobacterium tuberculosis, measles virus and so on. These bacteria and viruses can be spread by attaching to dust in the air. Through activities such as talking, coughing, and sneezing, people can spray pathogenic microorganisms in the mouth, throat, trachea, and lungs into the air through droplets and spread them to others. There may be more pathogenic microorganisms in indoor air, especially in poorly v...

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Problems solved by technology

[0006] There is following shortcoming in above-mentioned existing method: (1) because the sample to be tested is not easy to disperse into single cell completely, so the bacterium colony that forms is not all single bacterium colony, has a part to grow into by more than two cells, so plate colony count The test result will be lower than the actual number of colonies

(2) Some bacteria cannot be cultured and detected, or the culture medium can only select and cultivate some strains, thus limiting the scope of application of this method

(3) The detection period of this method is long, and the automatic operation of space separation culture is difficult to realize

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