Composition as well as preparation method and application thereof

A technology of composition and use, applied in the field of composition and its preparation, can solve the problems of no disease prevention, diabetes drugs can not play a role in cardiovascular and cerebrovascular diseases, diabetes can not play a good role, etc.

Inactive Publication Date: 2018-08-10
陈威
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, drugs for the treatment of cardiovascular and cerebrovascular diseases cannot play a good role in the treatment of diabetes, and drugs for the treatment of diabetes cannot directly play a role in the treatment of cardiovascular and cerebrovascular diseases
[0003] Most of the drugs currently used to treat these two major diseases respectively only play a palliative role in the treatment, and cannot play a substantial therapeutic role in restoring the normal quality and normal function of organs.
And it is

Method used

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  • Composition as well as preparation method and application thereof
  • Composition as well as preparation method and application thereof
  • Composition as well as preparation method and application thereof

Examples

Experimental program
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preparation example Construction

[0032] This embodiment also provides a preparation method of the above composition, which includes:

[0033] Step S1: extracting the mixture composed of natto and germinated soybeans with a buffer solution with a pH value of 4-5 to obtain an extract.

[0034] Natto is a soy product made from soybeans fermented by Bacillus natto (Bacillus subtilis). It is sticky, smells bad, and tastes slightly sweet. During the fermentation process, it produces a variety of physiologically active substances, including nattokinase .

[0035] Germinated soybeans refer to mature soybeans that germinate under suitable conditions. The germinated soybeans are rich in polypeptides as shown in SEQ ID NO: 1-4.

[0036] Preferably, when preparing the extract, the mixture of natto and germinated soybeans is mashed or ground to increase the dissolution rate of nattokinase and the polypeptide shown in SEQ ID NO: 1-4, thereby improving the extraction efficiency.

[0037] Further, the extraction temperatur...

Embodiment 1

[0050] The present embodiment provides a kind of composition, it comprises:

[0051] (1) The polypeptide shown in SEQ ID NO:1;

[0052] (2) Nattokinase.

[0053] The preparation method of this composition comprises:

[0054] a. Mix natto and germinated soybeans at a mass ratio of 1:1, mash them, mix them with a buffer solution with a pH value of 4 at a solid-to-liquid ratio of 1:5, and stir and extract at 15°C for 10 hours to obtain an extract.

[0055] b. Filtrate the extract with a microfiltration membrane with a molecular weight cut-off greater than 3000Da to obtain the filtrate;

[0056] c. Mix the obtained filtrate and table salt according to the mass volume ratio of 320g / L, then carry out salting-out at 15°C to obtain the salt-out product, and then use gel filtration or particle exchange to remove the salt in the salt-out product, After lyophilization, the composition is obtained.

Embodiment 2

[0058] The present embodiment provides a kind of composition, it comprises:

[0059] (1) the polypeptide shown in SEQ ID NO:2 and SEQ ID NO:3;

[0060] (2) Nattokinase.

[0061] The preparation method of this composition comprises:

[0062] a. Mix natto and germinated soybeans at a mass ratio of 1:2, mash them, mix them with a buffer solution with a pH value of 4 at a solid-to-liquid ratio of 1:4, stir and extract at 8°C for 12 hours to obtain an extract.

[0063] b. Filtrate the extract with a microfiltration membrane with a molecular weight cut-off greater than 3000Da to obtain the filtrate;

[0064] c. Mix the obtained filtrate and table salt according to the mass volume ratio of 380g / L, then carry out salting-out at 8°C to obtain the salt-out product, and then use gel filtration or particle exchange to remove the salt in the salt-out product, After lyophilization, the composition is obtained.

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Abstract

The invention provides a composition as well as a preparation method and application thereof. The composition comprises at least one polypeptide as shown in SEQ ID NO: 1 to 4, and nattokinase. The preparation method comprises the following steps: extracting a mixture comprising natto and germinal soybean by a buffer solution with the pH value being 4 to 5, so as to obtain an extracting solution; then filtering the extracting solution through a micro-filtration membrane with the molecular weight cut-off being greater than 3000Da, so as to obtain a filtered fluid; performing salting-out on the filtered fluid. The composition has the functions of reducing high blood pressure and blood viscosity, thereby having the effects of preventing and treating cardiovascular and cerebrovascular diseases;meanwhile the composition has the functions of recovering damaged pancreatic tissue and promoting regeneration, thereby being capable of preventing and treating diabetes; and the composition can be applied to the preparation of medicine or health care products for treating cardiovascular diseases and diabetes.

Description

technical field [0001] The invention relates to the field of medical and health products, in particular to a composition and its preparation method and application. Background technique [0002] Cardiovascular and cerebrovascular diseases and diabetes are closely related common diseases that seriously threaten human health. At present, drugs for treating cardiovascular and cerebrovascular diseases cannot play a good role in the treatment of diabetes, and drugs for treating diabetes cannot directly play a role in treating cardiovascular and cerebrovascular diseases. [0003] The medicines that treat these two major diseases respectively at present mostly only play a relieving effect in the treatment, and cannot play a substantive therapeutic effect to restore the normal quality and normal function of organs. And it is basically found that the disease has already occurred in the body and has obvious symptoms, such as the biochemical indicators of glucose metabolism and lipid ...

Claims

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Application Information

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IPC IPC(8): A61K38/16A61K38/48A61P9/00A61P3/10A61P9/12A61P7/02C12N9/54C07K14/415C07K1/36C07K1/34C07K1/30
CPCA61K38/168A61K38/482A61P3/10A61P7/02A61P9/00A61P9/12C07K14/415C12N9/54C12Y304/21062A61K2300/00
Inventor 陈正望陈威尧晨光
Owner 陈威
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