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A pertussis vaccine microneedle array and preparation method thereof

A microneedle array, pertussis technology, applied in pharmaceutical formulations, medical preparations containing active ingredients, antibody medical ingredients, etc., can solve the problem of inaccurate measurement, achieve excellent immune effect, avoid spillage, and operate safely. Effect

Active Publication Date: 2020-10-16
INST OF BIOMEDICAL ENG CHINESE ACAD OF MEDICAL SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, the current method of preparing microneedles for transdermal drug delivery cannot achieve accurate measurement
There is also no report on the combination of traditional pertussis vaccine with microneedle delivery technology

Method used

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  • A pertussis vaccine microneedle array and preparation method thereof
  • A pertussis vaccine microneedle array and preparation method thereof
  • A pertussis vaccine microneedle array and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] A method for preparing a pertussis vaccine microneedle array, comprising the steps of:

[0027] 1) Using a microneedle array mold, see figure 1 , the microneedle array mold includes a substrate 1, a rectangular groove 2 is arranged on the upper surface of the substrate 1, and a 10×10 array of tapered grooves 3 with downward pointed points is arranged on the bottom wall of the rectangular groove 2, and each cone The volume of the shaped groove 3 is 0.02 μL, and the total volume of the tapered groove is 2ul;

[0028] 2) Mix 1 mg / mL inactivated pertussis toxin aqueous solution and 10 mg / mL CpG adjuvant aqueous solution at a volume ratio of 1:1 to obtain solution 1, absorb 100 microliters and add it to rectangular groove 2 , stand still under vacuum conditions for 15 minutes, make the solution fill the conical groove 3, absorb excess solution other than the conical groove 3, and dry for 60 minutes;

[0029] 3) Smear a polyvinyl alcohol aqueous solution with a mass concent...

Embodiment 2

[0034] A method for preparing a pertussis vaccine microneedle array, comprising the steps of:

[0035] 1) with embodiment 1 step 1);

[0036] 2) Mix 0.1 mg / mL inactivated pertussis toxin aqueous solution and 10 mg / mL CpG adjuvant aqueous solution at a volume ratio of 1:1 to obtain solution 1, absorb 100 microliters and add it to the rectangular groove In 2, stand still under vacuum condition for 15 minutes, make the solution 1 fill the conical groove 3, absorb excess solution 1 other than the conical groove 3, and dry for 60 minutes;

[0037] 3) Same as embodiment 1 step (3).

[0038] Each pertussis vaccine microneedle array was loaded with pertussis toxin 0.1 μg and CpG adjuvant 10 μg.

Embodiment 3

[0040] A method for preparing a pertussis vaccine microneedle array, comprising the steps of:

[0041] 1) with embodiment 1 step 1);

[0042] 2) Mix 0.5 mg / mL inactivated pertussis toxin aqueous solution and 10 mg / mL CpG adjuvant aqueous solution at a volume ratio of 1:1 to obtain solution 1, absorb 100 microliters and add it to the rectangular groove In 2, stand still under vacuum condition for 15 minutes, make the solution 1 fill the conical groove 3, absorb excess solution 1 other than the conical groove 3, and dry for 60 minutes;

[0043] 3) Same as embodiment 1 step (3).

[0044] Each pertussis vaccine microneedle array was loaded with 0.5 μg of pertussis toxin and 10 μg of CpG adjuvant.

[0045] Experiment 2:

[0046] 24 female Balb / c mice, weighing 18-20g, were randomly divided into 4 groups, 6 in Example 1 group, and intradermally immunized with the pertussis vaccine microneedle array in Example 1; 6 in Example 2 group, respectively Intradermal immunization with th...

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Abstract

The invention discloses a preparation method of a pertussis vaccine micro needle array. The method comprises the following steps that (1) a micro needle array mold is used; the micro needle array moldcomprises a base plate; a rectangular groove is formed in the upper surface of the base plate; the bottom wall of the rectangular groove is provided with a conical groove with the downward array sharp end; (2) a pertussis toxin water solution and an adjuvant water solution are uniformly mixed to obtain a first solution; the first solution is added into the rectangular groove; still standing is performed under the vacuum conditions, so that the first solution is fully filled in the conical groove; the redundant first solution outside the conical groove is absorbed; drying is performed; (3) a high-molecular polymer solution is smeared in the rectangular groove; drying is performed; a mold with a conical bulge is removed; vacuum drying is performed. The pertussis vaccine micro needle array obtained by the method has the advantages that the dosage is accurate; the product specification is realized; the operation is safe.

Description

technical field [0001] The invention belongs to the technical field of vaccine administration equipment, and in particular relates to a pertussis vaccine microneedle array and a preparation method thereof. Background technique [0002] Pertussis is an acute respiratory infectious disease caused by Bordetella pertussis. It is generally caused by Bordetella pertussis (referred to as Bordetella pertussis). It can also be caused by Bordetella bronchiseptica and Bordetella parapertussis of the same genus. Bordetella pertussis is a Gram-negative bacillus that can produce some pathogenic substances, including pertussis toxin, tracheal cytotoxin, adenylyl cyclase toxin, heat-labile toxin, and endotoxin. Pertussis toxin can mobilize lymphocytes in the patient's lymphoid tissue to the surrounding blood and trachea, and the cytotoxin can specifically damage the ciliated epithelial cells of the trachea, leading to degeneration and necrosis. Pertussis patients, latent infections and car...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K39/10A61K9/00A61K39/39A61P31/04
CPCA61K9/0021A61K39/099A61K39/39A61K2039/55561A61P31/04
Inventor 张明明郭新东王晓莉孔德领朱丹丹马景景
Owner INST OF BIOMEDICAL ENG CHINESE ACAD OF MEDICAL SCI
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