42 results about "Tos ferina" patented technology

The invention provides a kit for detecting 10 respiratory tract infection pathogens and an application method thereof. The kit comprises primers and a TaqMan probe, wherein the primers are used for amplifying the 10 respiratory tract infection pathogens which include mycoplasma pneumoniae, chlamydia pneumoniae, legionella pneumophila, bordetella pertussis, rhinovirus, respiratory tract adenovirus, influenza A virus, influenza B virus, respiratory syncytial virus and parainfluenza virus. The application method includes: mixing 2 microliters of sample DNA / RNA co-extraction template, 20 microliters of RT-PCR buffer, 1 microliter of mixed enzyme liquid and 2 microliter of primer-probe mixed liquid to perform PCR amplification reaction. The kit uses the Taqman probe fluorescent PCR technology to detect the 10 common respiratory tract infection pathogens, a detection result can be obtained within 2 hours, the kit is high in specificity, and the sensitivity of the kit can reach 100copies / microliter.

The invention relates to a multi-component antigen cell-free pertussis vaccine for preventing Bordetella pertussis infection and a preparation method thereof. The cell-free pertussis vaccine comprises multi-component cell structure antigen which is obtained by cracking, separating, extracting and purifying thalli cells of Bordetella pertussis liquid cultures, and can further comprise multi-component cell secretion-type antigen which is obtained by separating, extracting and purifying the supernatant part of the Bordetella pertussis liquid cultures. The combined vaccine is suitable for infants, children, teenagers and adults for preventing the Bordetella pertussis infection.

This invention provides a composition of at least one Bordetella antigen and an effective adjuvant amount of interleukin-12 (IL-12), and uses thereof as a vaccine against Bordetella infection. Methods for using IL-12 as an adjuvant in combination with vaccines against Bordetella are also provided.

The present application relates to immunogenic compositions comprising a mixture of Bordetella (e.g., B. pertussis) antigens and an oil in water nanoemulsion. In particular, the invention provides immunogenic compositions comprising nanoemulsion and a combination of Bordetella (e.g., B. pertussis) antigens that have different functions, for example, combinations including B. pertussis adherence factors (adhesins), B. pertussis toxins or B. pertussis virulence factors. Vaccines, methods of treatment, uses of and processes to make a pertussis or whooping cough vaccine are also described. Compositions and methods of the present invention find use in, among other things, clinical (e.g. therapeutic and preventative medicine (e.g., vaccination)) and research applications.

The invention discloses honeycomb vinegar and a preparation method thereof and relates to a vinegar seasoning product. The vinegar comprises the following components in parts by weight: 0-8 parts of myotonin, 0-8 parts of black rice, 0-2 parts of red dates and 2-12 parts of honeycomb. The honeycomb vinegar is prepared by an acetic fermentation method of liquid backflow or solid fermentation. In the invention, the honeycomb, the myotonin, the black rice and the dates are used as raw materials, thus the vinegar has effective active substances of the honeycomb, the myotonin, the black rice and the dates. The product has good curative effect in the aspects of improving the immunity and treating the bronchitis, the rhinitis, the scirrhus, pain subcutaneous ulcer pyogenic infections, the benignprostatic hyperplasia, the arthritis, the hepatitis B, obstetrical and gynecological diseases, the impotence, the enuresis, the bone tuberculosis, stomach diseases, the pertussis and foot ringworms and has no toxic and side effects. Compared with the traditional table vinegar, the honeycomb vinegar has the advantages of gentle vinegar smell, pleasant fragrance and clear brownish red color. With the honeycomb vinegar, people can enjoy delicious food and also achieve the effect of medical health care.

Serogroup B meningococcus antigens can successfully be combined with diphtheria, tetanus and pertussis toxoids (“DTP”) to provide effective combination vaccines for protecting against multiple pathogens. These combinations are effective with a range of different adjuvants, and with both pediatric-type and booster-type DTP ratios. The adjuvant can improve the immune response which the composition elicits; alternatively, by including an adjuvant it is possible for the compositions to have a relatively lower amount of antigen while nevertheless having immunogenicity which is comparable to unadjuvanted combination vaccines.

PCT No. PCT / JP95 / 00830 Sec. 371 Date Oct. 13, 1995 Sec. 102(e) Date Oct. 13, 1995 PCT Filed Apr. 26, 1995 PCT Pub. No. WO95 / 29934 PCT Pub. Date Nov. 9, 1995To provide a method of efficiently separate protective components of Bordetella pertussis. On the basis of differences in adsorbability to calcium phosphate gel formed by adding calcium ions to a Bordetella pertussis culture in the presence of excess phosphate ions, protective components of Bordetella pertussis are separated from the Bordetella pertussis culture. Traditionally, protective components of Bordetella pertussis have been separated using different purification methods for the respective components. According to the present invention, the use of the same means of purification for all subject components makes it possible to purify each component with high efficiency and high recovery rate, an aspect very advantageous for industrial production. It is also possible to efficiently produce an improved purified pertussis component vaccine comprising an effective combination of pertussis filamentous hemagglutinin (FHA), pertactin (PRN, 69K-OMP), pertussis fimbriae (FIM) and pertussis toxin (PT).

The invention discloses a kit and a method for fluorescent quantitative detection of Bordetella pertussis, and provides a fluorescent quantitative reagent for detecting the Bordetella pertussis. The fluorescent quantitative reagent comprises a primer 1 for detecting the Bordetella pertussis, a primer 2 for detecting the Bordetella pertussis and a probe 1 for detecting the Bordetella pertussis. Experimental results prove that the kit and the detection method is based on Taqman fluorescent quantitative detection technique, pathogens of infection can be rapidly defined, detection expense is decreased, use efficiency of instruments is improved, and the method solves the problem of flux of clinical respiratory fungus infection pathogen detection and has important guide significance for clinical early diagnosis and treatment.

The invention relates to a herbal oral care preparation, and relates to the field of oral care. The herbal oral care preparation is prepared from the following raw materials: herba sarcandrae, cortexmoutan radicis, licorice root, fructus momordicae, radix zanthoxyli, herba portulacae, semen sterculiae lychnophorae, a fermented material, folium eriobotryae, fructus cnidii, herba rabdosiae rubescentis, flos jasmini nudiflori, flos osmanthi fragrans, glossy privet leaves, radix sophorae flavescentis, marigold root, flos lonicerae, fructus hyperici chinense, radix isatidis, cortex pericarpii granati, akebia stem, flos rosae rugosae, jasminum officinale root, and radix scutellariae. The herbal oral care preparation provided by the invention has the beneficial effects that the above herbal plants which are non-toxic and suitable for oral care are used as raw materials for efficacy, is purely natural, does not contain various additives and added materials, does not contain chemical components, can clear heat, detoxify, reduce swelling, reduce inflammation, slake thirst and help produce saliva, remove heat from the lung to relieve cough, remove heat to coll blood, harmonize the stomach and promote diuresis, diminish inflammation and relieve pain, is favorable for treating acute laryngitis, acute tonsillitis, tumor, diminishing inflammation, treating bronchitis, whooping cough, stomatitis, and dental ulcers, and relieving toothache.

The invention provides adsorbed acellular DTP-Sabin strain polio vaccine and a preparation method thereof. Every 100 ml of polio vaccine contains the following components: 3000-6000ugPN of whooping cough toxin, 3000-6000ugPN of filamentous hemagglutinin,1000-2000ugPN of pertactin, 1000-3000Lf of tetanus toxoid, 3000-6000Lf of diphtheria toxoid, 126-154mg of Al(OH)3, 3000-6000DU of sIPVI type, 5700-7100DU of sIPVII type, 4500-9000DU of sIPVIII type, 765-935mg of NaCl, 0-600mg of -2 phenoxyethanol and the balance of H2O. The vaccine can be used for preventing various target diseases, reducing the vaccination frequency, simplifying the immune procedure, improving the inoculation rate and reducing the risk of cross infection.

The present disclosure provides methods for determining whether a patient exhibiting pertussis-like symptoms will benefit from treatment, with therapeutic agents that inhibit Bordetella holmesii. These methods are based on detecting Bordetella pertussis, Bordetella parapertussis, and Bordetella holmesii in a biological sample by assaying for the presence of the IS481, IS 1001, and hIS1001 target repeat elements, respectively. Kits for use in practicing the methods are also provided.

The invention provides a screening method and content measurement method for bordetella pertussis tracheal cytotoxin in pertussis or diphtheria-pertussis-tetanus vaccine. The content detection methodcomprises the following steps of (1) drawing a standard working curve, particularly weighting an appropriate amount of bordetella pertussis tracheal cytotoxin reference substance, preparing a standardworking solution, analyzing the standard working solution by high performance liquid chromatography tandem mass spectrometry, obtaining a chromatogram of the reference substance, and obtaining the standard working curve according to a relation between solution concentration and the corresponding chromatographic area; and detecting a sample, particularly taking and adding a to-be-measured sample to acetonitrile with the same volume, performing centrifuging after uniform mixing, taking a supernatant, analyzing and detecting the supernatant by the high performance liquid chromatography tandem mass spectrometry, and obtaining content of the bordetella pertussis tracheal cytotoxin of a to-be-measured constituent according to the response peak area of the to-be-measured constituent and the standard working curve. The method has ultrahigh sensitivity, and the limitation of the method is lower than the limitation required by European Pharmacopoeia by 1,409 times.

The invention discloses a traditional Chinese medicine preparation for treating pertussis by reducing phlegm and stopping coughing and a preparation method of the traditional Chinese medicine preparation. The traditional Chinese medicine preparation is prepared from the following main raw materials in parts by weight: 9-15 parts of radix astragali, 11-16 parts of acalypha australis, 7-12 parts of herba andrographitis, 9-14 parts of morinda parvifolia, 11-15 parts of uncaria rhynchophylla, 8-13 parts of polytrichum commune, 9-15 parts of root of white flowered bitterness, 6-10 parts of root of orixa, 4-9 parts of radix stemonae, 9-12 parts of radix asteris, 11-17 parts of white orchid, 8-16 parts of faber bauhinia branchlet root, 12-15 parts of caulis bambusae in taeniam, 10-17 parts of centipeda minima, 8-14 parts of rhizoma phragmitis, 5-8 parts of garlic, 10-14 parts of aleuritopteris argentea and 8-12 parts of arisaema japonicum. The traditional Chinese medicine preparation is mild in medicine property, free of side effects, easily acceptable to infants with pertussis, free of adverse effects on growth and development of the infants with pertussis and applicable to treatment on the infants with pertussis.

The invention provides a pertussis PRN protein monoclonal antibody and an application thereof, which belong to the biological product field. The monoclonal antibody is prepared by taking PRN protein in bordetella pertussis as immunogen immune mice, the obtained two monoclonal antibodies are respectively as, an amino acid sequence of a light-chain variable region is shown in a SEQ ID No.1, the amino acid sequence of a heavy-chain variable region is shown in a SEQ ID No.2, the amino acid sequence of the light-chain variable region is shown in a SEQ ID No. 3, and the amino acid sequence of the heavy-chain variable region is shown in a SEQ ID No.4. The provided monoclonal antibody enables no cross reaction with bordetella pertussis other protein and other antigen and pathogen, has the advantages of high specificity and high sensitivity for detecting the pertussis PRN components, can accurately detect PRN content of pertussis vaccine in a sample, and can be widely used in vaccine and clinical detection.

The invention provides a separation and purification method for a pertussis antigen. The separation and purification method comprises the steps of with diatomite and hydroxylapatite as column chromatography materials, after concentrating, regulating and conducting a bordetella pertussis fermentation solution, enriching the pertussis antigen by using the diatomite, and then, purifying by using the hydroxylapatite to obtain pertussis toxin (PT) and filamentous hemagglutinin (FHA). Proven through SDS-PAGE electrophoresis detection, the purities of both a PT protein and an FHA protein are higher than 95%. The method provided by the invention is simple in operation and capable of obtaining a high-purity antigen, not only greatly reducing the production cost, but also remarkably improving the quality of a product and increasing the yield of the product so as to be the simple and rapid separation and purification method for the pertussis antigen; in addition, the economic benefit is high, and the market application prospect is wide.

The invention discloses a traditional Chinese medicine for treating pertussis. The traditional Chinese medicine is prepared by the following raw medicinal materials in parts by weight: 9 to 12 parts of baical skullcap root, 11 to 14 parts of amur honeysuckle, 9 to 15 parts of common nepenthes, 10 to 16 parts of henry sarmentose fig, 12 to 17 parts of root of hairy asiabell, 9 to 13 parts of acmella ciliate, 7 to 12 parts of fourleaf peperomia herb, 10 to 15 parts of common cyanotis root, 11 to 14 parts of dwarf lilyturf tuber, 9 to 17 parts of common bluebeard herb, 5 to 9 parts of pimpinella, 9 to 14 parts of Chinese cinquefoil herb, 11 to 16 parts of wintersweet flower, 7 to 12 parts of smallflower milkwort herb with root, 9 to 16 parts of purslane herb, 11 to 15 parts of common coltsfoot flower, 10 to 17 parts of parnassia palustris, and 8 to 16 parts of loquat leaf. The traditional Chinese medicine has the beneficial effects that the effects of clearing lungs and eliminating phlegm, relieving cough and asthma, invigorating qi and tonifying kidneys, and dispersing livers and promoting blood circulation are realized; the materials are traditional Chinese medicinal materials, so any toxic and side effect is avoided; the obtaining of medicinal materials is easy, the cost of medicinal materials is low, and the burden of a patient is reduced.

The invention belongs to the field of medical services, and relates to a cloud computing technology, in particular to a cloud computing medical system based on an air purifier, which comprises a cloud platform; the cloud platform is in communication connection with a processor, and the processor is in communication connection with an air purification module, a medicine management module, a parking planning module, an appointment registration module, a storage module and a controller; the air purification module is used for detecting and analyzing hospital air quality. The specific detection and analysis process comprises the following steps: the content of mycobacterium tuberculosis and the content of bordetella pertussis in air are obtained; a tubercle bacillus content threshold value and a bordetella pertussis content threshold value are obtained through a storage module, and the tubercle bacillus content and the bordetella pertussis content are compared with the tubercle bacillus content threshold value and the bordetella pertussis content threshold value respectively. According to the invention, the hospital air quality can be detected and analyzed, and the situation that a large number of harmful bacterial colonies contained in the hospital air are inhaled by the human body, and harm and injury are caused to the human body is avoided.

The invention relates to a production method of semen lepidii and fructus jujubae lung purging granules, in particular to production of semen lepidii and fructus jujubae lung purging granules from twobulk medicine materials of fructus jujubae and semen lepidii through technologies of pretreatment, decoction, decotion with stir-fried semen lepidii wrapped, filtering, concentration, drying, granulation and the like, and belongs to the field of traditional Chinese medicine preparations. The obtained semen lepidii and fructus jujubae lung purging granules have the efficacy of purging the lung, inducing diuresis, descending qi and dissolving phlegm, and are used for treating respiratory system disease of pulmonary heart disease, emphysema, wet pleurisy, lobar pneumonia, bronchial asthma, chronic bronchitis, pertussis and the like, the recipe is simple, however, the semen lepidii and fructus jujubae lung purging granules have significantly-curative and excellent effects. According to production of the semen lepidii and fructus jujubae lung purging granules from the two bulk medicine materials of the fructus jujubae and the semen lepidii through the technologies of pretreatment, decoction, decotion with the stir-fried semen lepidii wrapped, filtering, concentration, drying, granulation and the like, by conducting dosage form reform and innovation on traditional Chinese medicine decotion, the problems that production, taking and carrying are inconvenient, and the like can be effectively solved, the production method of the semen lepidii and fructus jujubae lung purging granules isscientific and reasonable, the materials for efficacy are kept furthest, and large-scale production is easy, so that the production method of the semen lepidii and fructus jujubae lung purging granules has significant economic benefits and application prospects.

The invention relates to a liquid vacuum composition which comprises diphtheria toxoid, tetanus toxoid, pertussis toxins, filamentous hemagglutinin, pertactin, inactivated poliovirus, type-b haemophilus influenzae capsular polysaccharide-protein conjugate and meningococcus capsular polysaccharide-protein conjugate, wherein the inactivated poliovirus comprises type-I, type-II and type-III poliovirus Sabin strains. By adopting the liquid vacuum composition provided by the invention, security risks caused by freeze-drying processes and limits of production capacities can be avoided, and meanwhile, a tedious redissolution process needed before immunization of a freeze-dried vacuum is avoided. The liquid vacuum composition provided by the invention is capable of preventing six infectious diseases simultaneously, so that the workload of inoculation and the burden of children can be greatly reduced.

The invention relates to a method for preparing an acellular pertussis vaccine, comparing: ultrafiltrating and condensing bordetella pertussis culturing supernatant, to obtain a concentrated solution; carrying out cation exchange column chromatography on the concentrated solution, to separate out pertussis toxin (PT) and filamentous hemagglutinin (FHA); optionally, carrying out multi-combination anion exchange chromatography on a flowing-through liquid obtained in a step (2), to separate out pertactin (Prn); PT is individually used to prepare the acellular pertussis vaccine, or PT is mixed with FHA and / or Prn to prepare the acellular pertussis vaccine.

The invention discloses a respiratory tract infection treating drop for pediatric nursing. The drop is mainly prepared from, by weight, 3-9 parts of mint, 15-30 parts of honeysuckle, 10-30 parts of radix stemonae (preferentially, 10.5-29.5 parts of radix stemonae), 3-9 parts of dried tangerine peel, 3-10 parts of herba centellae, 15-30 parts of cortex pseudolaricis, 15-30 parts of common jujube bark, 10-20 parts of lalang grass rhizome, 10-20 parts of acorus calamus, 5-9 parts of Polygonum cynanchoides Hemsl. and 5-10 parts of pear juice. The drop has a remarkable effect on treating pediatric pertussis, pneumonia and upper respiratory infection and moistening lung to arrest cough, has functions of clearing away heat and removing phlegm, and killing bacteria and eliminating inflammation, and has an effect on treating rhinitis. Local frequently-seen medicine materials are selected for use, price is low, and economic burdens of patients are reduced.

The invention discloses a traditional Chinese medicinal composition for treating pertussis. The traditional Chinese medicinal composition comprises, by weight, 10-30 parts of Momordica Grosvenori, 10-20 parts of Solidago Decurrens, 5-15 parts of Radix Asteris, 10-20 parts of Rhizoma Imperatae, 10-20 parts of Cortex Mori, 5-15 parts of Picris Hieracioides, 10-20 parts of Scaphium Scaphigerum, 10-20 parts of Codonopsis Pilosula, 5-15 parts of Parnassia, 10-20 parts of Pyrrosia Lingua, 10-20 parts of Herba Aletridis, 10-20 parts of lilies, 5-15 parts of Fritillaria Cirrhosa, 10-20 parts of Herba Andrographitis, 10-20 parts of Radix Pseudostellariae, 10-20 parts of cotton rose hibiscus leaves, 5-15 parts of Herba Polygalae Japonicae, 10-20 parts of Rhynchelytrum Repens, 10-20 parts of Piper Betle, 10-20 parts of Crotalaria Ferruginea and 5-15 parts of liquorice. The traditional Chinese medicinal composition has the advantages that the composition has the effect of clearing away heat and toxic materials, expelling flatulence, reducing phlegm, ventilating lung, relieving cough, promoting blood circulation and promoting secretion of saliva, the curative effect of treating the pertussis is remarkable and reliable, medicinal herb resources are extensive, and the traditional Chinese medicinal composition is simple and convenient to prepare and free of toxic and side effect.

A method of reducing or preventing the development of airway inflammation in a subject comprising the step of infecting the subject's respiratory tract with an amount of a composition comprising a pharmaceutically acceptable carrier and sufficient to colonize the subject Respiratory attenuated B. pertussis adhesin-deficient live bacteria of the genus Bordetella. The step of infecting the subject with the live attenuated pertactin-deficient Bordetella bacterium results in reducing or preventing the development of airway inflammation in the subject.

The invention discloses a traditional Chinese medicament for treating pertussis. The traditional Chinese medicament is prepared from the following main raw materials in parts by weight: 13-17 parts of Codonopsis pilosula, 10-14 parts of Polygonum chinense, 11-15 parts of potentilla chinensis, 7-12 parts of concretio silicea bambusae, 9-15 parts of fourleaf peperomia herb, 11-16 parts of radix curcumae, 6-9 parts of airpotato yam rhizome, 10-15 parts of Acmella ciliate, 12-16 parts of radix ophiopogonis, 7-15 parts of Chinese milkwort, 10-17 parts of chimonanthus praecox flower, 8-15 parts of Scutellaria baicalensis, 11-14 parts of Parnassia palustris, 9-13 parts of herba portulacae, 12-16 parts of common bluebeard herb, 6-9 parts of herb of diversifolious pimpinella, 10-14 parts of Cyanotis arachnoidea and 8-13 parts of aster fastigiatus fisch. After differentiated compatibility of various raw materials, the traditional Chinese medicament has the efficacies of relieving cough and reducing sputum, dispelling wind and subsiding swelling, clearing away heat and toxic materials as well as diffusing the lung and calming adverse qi and has a remarkable curative effect on treatment of the pertussis.

A method of reducing or preventing the development of airway inflammation in a subject includes the step of infecting the respiratory tract of a subject an amount of a composition including a pharmaceutically acceptable carrier and live attenuated pertactin-deficient Bordetella bacteria sufficient to colonize the respiratory tract of the subject. The step of infecting the subject with the live attenuated pertactin-deficient Bordetella bacteria results in reduction or prevention of the development of airway inflammation in the subject.

The invention relates to a suspension microbead array system for detecting common pathogens of the respiratory tract infection. The system detects influenza A virus, influenza B virus, parainfluenza virus, human metapneumovirus, rhinovirus, bocavirus, adenovirus, respiratory syncytial virus, mycoplasma pneumoniae, moraxella catarrhalis, haemophilus influenza, streptococcus pneumoniae and bordetella pertussis in one experiment. The suspension microbead array system finishes the detection of 8 viruses, 4 bacteria and 1 mycoplasma in one experiment, prevents leak detection to the greatest extent, and is beneficial for the targeted clinical treatment; the cost of reagents, if divided on the detection of each index, is greatly lowered, so that the medical cost is reduced.

The invention discloses honeycomb vinegar and a preparation method thereof and relates to a vinegar seasoning product. The vinegar comprises the following components in parts by weight: 0-8 parts of myotonin, 0-8 parts of black rice, 0-2 parts of red dates and 2-12 parts of honeycomb. The honeycomb vinegar is prepared by an acetic fermentation method of liquid backflow or solid fermentation. In the invention, the honeycomb, the myotonin, the black rice and the dates are used as raw materials, thus the vinegar has effective active substances of the honeycomb, the myotonin, the black rice and the dates. The product has good curative effect in the aspects of improving the immunity and treating the bronchitis, the rhinitis, the scirrhus, pain subcutaneous ulcer pyogenic infections, the benignprostatic hyperplasia, the arthritis, the hepatitis B, obstetrical and gynecological diseases, the impotence, the enuresis, the bone tuberculosis, stomach diseases, the pertussis and foot ringworms and has no toxic and side effects. Compared with the traditional table vinegar, the honeycomb vinegar has the advantages of gentle vinegar smell, pleasant fragrance and clear brownish red color. With the honeycomb vinegar, people can enjoy delicious food and also achieve the effect of medical health care.

The invention discloses a healthcare decoction capable of relieving pertussis in the period of spasmodic cough, and relates to the technical field of healthcare traditional Chinese medicine preparations. The healthcare decoction capable of relieving the pertussis in the period of the spasmodic cough is prepared from the following crude drugs in parts by weight: 10-20 parts of stiff silkworms, 12-25 parts of cicada slough, 8-15% of linden leave extract, 2-5 parts of bistort extract, 15-30 parts of indigo naturalis, 8-15 parts of radix asteris, 6-15 parts of centella asiatica and 3-10 parts of liquorice. The healthcare decoction capable of relieving the pertussis in the period of the spasmodic cough disclosed by the invention has the advantages of reasonable combination of the components, and capability of clearing away heat, dispersing lung, realizing spasmolysis, preventing phlegm from forming, stopping coughing, relieving discomfort of an infant in the period of the spasmodic cough, and calming and soothing nerves, and has obvious effects on clinical application to the pertussis of the infant in the period of the spasmodic cough.