Chlorella preservation method

Abstract

The invention provides a chlorella preservation method, and belongs to the technical field of aquaculture. The method includes the steps: culturing chlorella to a logarithmic phase; adding millionth-to-thousandth biological flocculants into logarithmic phase chlorella liquid without sundry fungus pollution according to volume dose; stirring mixture for 5-30 minutes; performing flocculation and sedimentation for 2-8 hours; placing acquired chlorella concentrated solution into a triangular flask; adding 10%-30% of glycerin until the concentration of the chlorella is 5*10<7>-1*10<9> pieces / mL; sealing the chlorella by the aid of a sealing film; placing the sealed chlorella into a refrigerator to refrigerate the sealed chlorella at the temperature of 4 DEG C. The method has the advantages thatthe method is simple to operate, special devices are omitted, sundry fungus pollution can be effectively avoided, preservation time is long, activity retention rate is high and the like. The method can be used for chlorella preservation of chlorella production enterprises.

Description

technical field [0001] The invention relates to the technical field of aquaculture, in particular to a method for preserving chlorella. Background technique [0002] There is no animal or plant in the world that can grow 4 times every 20 hours like chlorella, mainly due to the growth factor of chlorella. Chlorella Growth Factor (CGF), also known as chlorella essence, is a cell active substance, including amino acids, nucleic acids, polysaccharides, polypeptides, proteins, enzymes, vitamins, minerals, and is called "hormone-like". ". Chlorella growth factor has the functions of: activating lymphocytes, enhancing the immune ability of the human body, activating human cells, promoting the growth and development of children, resisting the invasion of foreign diseases, containing many nucleoproteins required for repairing human organs, and can treat poisoning such as organic substances and heavy metals; Prevention and treatment of gastric ulcer, high blood pressure and cardiova...

AI Technical Summary

Problems solved by technology

The preservation time of the solid plate preservation method is often short (usually 1-2 months), and the operation is complicated. Since the surface of the medium is easy to dry, it needs to be subcultured regularly. Frequent subculture not only increases the labor cost and the risk of pollution, but also easily leads to algae Degeneration or loss of good traits

Although the cryopreservation method has a long storage time, it requires expensive equipment, consumes a lot of energy, and has harsh storage conditions, so it is difficult to be widely used

[0004] Invention patent 201711100019.3 proposes a method of preserving chlorella using agar plates. This method requires the preparation of soil extracts and the use of 96-well plates, so it is not suitable for the preservation of chlorella cultivation enterprises

201010222005.0 proposed to pack the algae cell suspension in ampoules, pre-freeze the microalgae, then freeze it, and vacuumize the freeze-dried algae powder. This method can prolong the storage period of the microalgae, but the operation is complicated and the preservation is expensive. , not suitable for the preservation of strains in small and medium-sized breeding enterprises

[0005] Aiming at the defects of short storage time, cumbersome operation, and expensive equipment required for the existing microalgae strain preservation methods, this method proposes a method that is simple to operate, does not require expensive equipment, has good preservation effect, and is suitable for small and medium-sized enterprises to preserve strains. Methods

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