Antibacterial protein film as well as preparation method and application thereof
An antibacterial protein and soybean protein isolate technology, applied in the field of antibacterial film, can solve the problems of unstable performance and inability to distribute inorganic nano materials evenly, and achieve the effects of high performance stability, good ultraviolet shielding and good uniformity
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[0030] In addition, the present invention also provides a method for preparing the above antibacterial protein film, which specifically includes the following steps:
[0031] 1) Weighing raw materials, including soybean protein isolate, nano zinc oxide, plasticizer, dispersant and solvent water;
[0032] 2) Add nano-zinc oxide and dispersant to the solvent water, then add soybean protein isolate, heat and stir, and disperse; wherein, in order to fully mix the materials, the mixing operation of raw materials is generally carried out under magnetic stirring, and the magnetic stirring speed is generally not More than 1400r / min, and add slowly when adding soybean protein isolate; heating and stirring operation usually adopts constant temperature water bath heating under the condition of 30-100 ℃, and magnetic stirring at the same time for 10-60min, and the stirring speed generally does not exceed 1400r / min;
[0033] 3) Cool to room temperature, filter to take the filtrate, then ad...
Embodiment 1
[0038] (1) 0.01g nano-zinc oxide and 0.3g Tween are added to 97mL water to obtain a mixed solution;
[0039] (2) Add 2 g of soybean protein isolate to the mixed solution obtained in step (1), heat in a constant temperature water bath at 30° C., and magnetically stir for 10 minutes at the same time;
[0040] (3) filter and get the filtrate after being cooled to room temperature;
[0041] (4) Get 1.5g of glycerin and join in the filtrate gained in step (3), and magnetically stir for 20min to obtain a film-forming mixed solution;
[0042] (5) Pour the film-forming mixed liquid obtained in step (4) onto a plexiglass plate, and then place it in a drying oven at 70° C. for 5 hours to dry.
Embodiment 2
[0044] (1) Under magnetic stirring, 1.0 g of nano-zinc oxide and 1.5 g of Tween were added to 90 mL of water to obtain a mixed solution;
[0045] (2) Add 4 g of soybean protein isolate to the mixed solution obtained in step (1), heat in a constant temperature water bath at 50° C., and magnetically stir for 20 min at the same time;
[0046] (3) filter and get the filtrate after being cooled to room temperature;
[0047] (4) 4g glycerol is added in the filtrate gained in step (3), and magnetically stirred for 10min to obtain a film-forming mixed solution;
[0048] (5) Pour the film-forming mixture obtained in step (4) onto a plexiglass dish, and put it in a drying oven at 50°C for 4 hours to dry.
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