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Method and apparatus for isotope ratio mass spectrometry

A technology of isotope ratio mass spectrometry and isotope ratio, which is applied in the field of mass spectrometry and can solve the problems of not reaching the precision and accuracy of isotope ratio measurement, and the complexity of the system.

Active Publication Date: 2022-02-18
THERMO FISHER SCI BREMEN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the step of removing the sample from the main stream into the loop and then filling the mass spectrometer has not achieved the level of precision and accuracy typically required for isotope ratio measurements
In addition, the system is complex and requires an additional sample loop and a second pump system

Method used

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  • Method and apparatus for isotope ratio mass spectrometry
  • Method and apparatus for isotope ratio mass spectrometry
  • Method and apparatus for isotope ratio mass spectrometry

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0124] Example 1 - Injection Measurement (Comparative Example)

[0125] The samples listed in Table 1 listed in 10 μg / ml were prepared in the sample solution in methanol / water 50:50 (V: V) and directly injected to Standard Saimo Technology from the Saiman Fortress. Track well quality spectrometer. Schematic shown in instrument Figure 4 In, the instrument is a preferred mass spectrometer for use in the present invention. The mass spectrometer 200 includes an electrospray ion source 202 that generates ions, the ions to enter the RF lens 204 before guiding the ions 208 by the active beam guide 208. The mass isolation window can be set to the downstream ion trap (C well) 210 by the material of the material, wherein the ion is injected into the orbital well quality analyzer 212 can accumulate before quality analysis. If desired, ion emission can be transmitted through the C-well 210 to downstream, and at a higher energy collision decomposition unit, the ion can be degraded before...

example 2

[0138] Example 2 - LC / MS measurement without flow (comparative example)

[0139] Samples listed in Table 1 of 10 μg / mL (+ / - 0.02 μg / mL) were used in experiments in the solution of methanol / water 20:80. Such as figure 2 The LC / MS system schematically showed is used with the following LC settings:

[0140] - Saimi Fitt Technology with automatic sampler and tetram pump system

[0141] -10μL injection (corresponding to 100 ng injection)

[0142] - Saimo Fei Hotel AccuCore AQ column; 2.6mm particle size

[0143] - Solvent A: Water, 0.1% formic acid (FA), 2 mM acetate

[0144] - Solvent B: methanol, 0.1% Fa, 2 mM acetate

[0145] - Solvent gradient is 100% A (0 to 0.5 min); 50% A / 50% B (0.5 to 3.0 minutes); 20% A / 80% B (3.0 to 3.3 minutes); 100% A (3.3 to 5 minutes) . Figure 8 Show the solvent joint gradient for the experiment.

[0146] Use the following parameters to operate the mass spectrometer:

[0147] - Quality scan / isolation range 190 to 200m / z

[0148] - Diso...

example 3

[0161] Example 3: LC / MS measurement with low flow

[0162] Samples listed in Table 1 listed in Table 1 of 10 μg / ml (+ / - 0.02 μg) were used in experiments in the sample solution of Table 1 of methanol / water 20:80. Such as image 3 The LC / MS system schematically showed is used with the following LC settings:

[0163] - Saimi Fitt Technology with automatic sampler and tetram pump

[0164] -10μL injection (corresponding to 100 ng injection)

[0165] - Saimo Fei Hotel AccuCore AQ column; 2.6MM ×, 2.6mm particle size

[0166] - Solvent A: Water, 0.1% Fa, 2MM ammonium acetate

[0167] - Solvent B: methanol, 0.1% Fa, 2 mM acetate

[0168] - Gradient 100% A (0 to 0.5 minutes); 50% A / 50% B (0.5 to 8.5 minutes); 20% A / 80% B (8.5 to 9.0 minutes); 100% A (9.0 to 11.0 minutes) Cascade gradient)

[0169] - Split valve in t = 2.75 minutes and T = 8.00 minutes

[0170] Figure 11 Show the solvent joint gradient for the experiment. In this experiment, a reduced flow setting is used. Su...

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Abstract

The present application relates to methods and apparatus for isotope ratio mass spectrometry. Isotope ratio mass spectrometry comprising: flowing through a separation device a liquid mobile phase containing a sample comprising at least one molecular species having an isotope ratio to be determined; Set or reduce the flow rate of the liquid mobile phase flowing through the separation device over time to achieve the desired isotope ratio accuracy; at least for the at least one molecular species that has emerged from the separation device while setting or reducing the flow rate performing mass analysis; determining at least one isotope ratio of said at least one molecular species from mass peak intensities of at least two isotopologues of said at least one molecular species according to said mass analysis, said mass analysis passing being sufficiently high to resolve between The mass resolution of the two most abundant mass peaks of the nominal mass of at least one of the isotopologues is performed.

Description

Technical field [0001] The present invention relates to the field of mass spectrometry. The invention relates particularly to isotope specific mass spectrometry (Irms), and more particularly to IRMs coupled to liquid chromatography. The present invention provides both methods and equipment. Background technique [0002] Conventionally, accurate and precision isotope measurements are carried out on a magnetic sector mass spectrometer, particularly on a magnetic sector mass spectrometer that uses a multi-collector while detecting isotope. Prior to analysis, the samples generally undergo oxidation, pyrolysis, and / or also generate gaseous molecules at high temperatures, such as CO. x NO x , N 2 H 2 O and SO 2 One or more of the middle (x = 1 or 2). The gas is then introduced into the isotope ratio in mass spectrometer for isotope analysis. In the isotope than the mass spectrometer, the gas is ionized and, for example, by comparing the output of different collectors to measure the r...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/62G01N30/72G01N30/86H01J49/00H01J49/04H01J49/28H01J49/38H01J49/40
CPCG01N27/62G01N30/8658G01N2030/324H01J49/0031G01N30/7233H01J49/0036H01J49/38H01J49/40H01J49/282H01J49/0459
Inventor J·格力普-拉敏
Owner THERMO FISHER SCI BREMEN
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