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Nematicidal microbial organic fertilizer and its preparation method and application

A microbial organic fertilizer and nematicide technology, applied in the directions of microorganism-based methods, biochemical equipment and methods, and botanical equipment and methods, can solve problems such as threats to safe production of wheat, yellowing of leaves, and dwarfing of plants, etc. Inhibit vegetable root-knot nematode and wheat cyst nematode, promote growth, and improve the effect of agricultural production

Active Publication Date: 2021-01-26
INST OF PLANT PROTECTION HENAN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Wheat cyst nematode is also a new wheat soil-borne disease that has occurred in my country in recent years. The pathogenic nematode mainly damages the roots of wheat, invades the root system of wheat and reproduces in it to form bulbous root knots, thereby inhibiting the absorption of nutrients by plants , resulting in yellowing of the aboveground leaves of wheat, dwarfing of plants, resulting in reduced yield, which seriously threatens the safe production of wheat in my country

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Embodiment one: the preparation of microbial organic fertilizer

[0039] 1. Cultivation of Methylotrophic Bacillus

[0040] (1) Methylotrophic Bacillus was inoculated into beef extract peptone solid medium, and cultured at 30°C for 32 hours to obtain activated strains;

[0041] (2) Inoculate the activated methylotrophic bacillus into the first liquid culture medium, and cultivate it for 6-12 hours at a temperature of 28-35°C and a rotation speed of 150-210 rpm to obtain a first-class seed liquid;

[0042] Among them, the formula of the first liquid medium is beef extract 0.5%, peptone 1.5%, fish meal 0.3%, sodium chloride 0.6%, pH7.2;

[0043] (3) Inoculate the primary seed solution into the seed fermentation tank containing the liquid fermentation medium, the initial pH value is 7.2-7.5, and cultivate for 6-12 hours at 28-35°C and the rotation speed is 170-220 rpm. Prepare secondary seed liquid;

[0044] Among them, the formula of the liquid fermentation medium is 2...

Embodiment 2

[0048] Embodiment two: the preparation of microbial organic fertilizer

[0049]The difference from Example 1 is that peat powder, humic acid and amino oligosaccharin with a fineness of 0.1mm are taken, stirred at 40rpm for 25min; then the methylotrophic bacillus liquid concentrate is added, and stirred at 65rpm for 25min; , the weight ratio of peat powder, humic acid, amino oligosaccharin, and methylotrophic bacillus concentrate is: 88:7:0.06:4.94; at 45°C, dry to a moisture content of 7%; The number of live bacteria was measured by plate counting method, and the number of live bacteria was 650 million / g; it was pulverized by a pulverizer and passed through a 50-mesh sieve to obtain microbial organic fertilizer.

Embodiment 3

[0050] Embodiment three: the preparation of microbial organic fertilizer

[0051] The difference from Example 1 is that peat powder, humic acid and amino oligosaccharin with a fineness of 0.2mm are taken, stirred at 50rpm for 30min; then the methylotrophic bacillus liquid concentrate is added, and stirred at 80rpm for 30min; , the weight ratio of peat powder, humic acid, amino oligosaccharin, and methylotrophic bacillus concentrate is: 94:5:0.01:0.99; at 50°C, dry to a moisture content of 10 %; use the plate counting method to measure the number of viable bacteria, and the amount of viable bacteria is 100 million / g; use a pulverizer to crush, pass through a 60-mesh sieve, and obtain microbial organic fertilizer.

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Abstract

The invention discloses a nematode-killing microbial organic fertilizer, which contains methylotrophic bacillus CGMCC NO.10834 concentrated bacterial liquid, amino oligosaccharins, humic acid and peat powder; the methylotrophic bacillus The ratio by weight of the bacillus concentrated bacterial liquid, the amino oligosaccharin, the humic acid and the peat powder is: 0.1-10: 0.01-0.1: 5-10: 80-94. The preparation method of the microbial organic fertilizer is as follows: take peat powder with a fineness of 0.05-0.2mm, humic acid and amino-oligosaccharin, stir at 30-50rpm for 20-30min; Stir for 20-30 minutes; adjust the pH value to 5.5-8.0; dry at 40-50°C until the moisture content is 5-10%; pulverize with a pulverizer and pass through a 40-60 mesh sieve to obtain microbial organic fertilizer. The nematicide microbial organic fertilizer is applied in the prevention and treatment of nematode diseases and crop yield increase. The microbial organic fertilizer of the invention has dual functions of medicine and fertilizer, has the characteristics of high efficiency, greenness and safety, good stress resistance and low production cost.

Description

technical field [0001] The invention relates to the technical field of fertilizer preparation, in particular to a nematicidal microbial organic fertilizer and a preparation method and application thereof. Background technique [0002] With the adjustment of agricultural planting structure in our country, the cultivation area of ​​vegetables is expanding continuously, and the phenomenon of continuous cropping and heavy cropping is common, which provides favorable conditions for the occurrence and development of vegetable root-knot nematodes. [0003] Root-knot nematodes mainly harm the lateral roots and fibrous roots of vegetables, forming nodular root knots of different sizes. The root knots are milky white in the early stage and light brown in the later stage. When the disease is severe, the root knots are connected to each other into beads. Many fibrous roots are formed, and the roots are in the shape of a mass, and the white pear-shaped female adults can be seen when the ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C05G3/60C05G3/00C05G3/80C12N1/20A01G13/00C12R1/07
CPCA01G13/00C05C11/00C05G3/00C12N1/20C05G3/60C05G3/80C05F11/02C05F11/08
Inventor 张洁杨丽荣夏明聪全鑫薛保国孙润红武超杨艳汤蒙蒙
Owner INST OF PLANT PROTECTION HENAN ACAD OF AGRI SCI
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