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Method for measuring contents of multiple components of taxillus sutchuenensis

A determination method and multi-component technology, applied in the field of chemistry, can solve the problems of differences in curative effects, inability to fully and clearly evaluate the quality of Sangjisheng medicinal materials, and achieve the effects of simple operation, shortened detection time, and improved work efficiency

Inactive Publication Date: 2018-08-17
GUANGXI UNIV OF CHINESE MEDICINE
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  • Application Information

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Problems solved by technology

[0004] At present, the quality control of Sangjizi medicinal materials is relatively simple, and only the content of quercetin and / or quercetin can not be fully and clearly evaluated for the quality control of Sangjisheng medicinal materials. Lili et al., "China Health Industry", 2015, 12(27): 126-128) disclosed that the HPLC method was used to determine the content of quercetin in Mulberry, which can only be used to distinguish Mistletoe from Mistletoe
The literature "Comparison of Extraction Methods for the Determination of Quercetin and Quercetin Content in Mulberry by RP-HPLC" (Su Benwei et al., "Guangxi Traditional Chinese Medicine", 2012,35(4):53-55) discloses the simultaneous determination of Mulberry The method of quercetin and quercetin content in the medium can only detect the two components of quercetin and quercetin, and the curative effect in clinical application is different due to the different content of agaricin in different host sources.

Method used

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  • Method for measuring contents of multiple components of taxillus sutchuenensis
  • Method for measuring contents of multiple components of taxillus sutchuenensis
  • Method for measuring contents of multiple components of taxillus sutchuenensis

Examples

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Embodiment 1

[0025] Example 1: High-performance liquid chromatography content determination of three active ingredients, quercetin, quercitrin, and galactoside contained in Mulus mulberry.

[0026] 1. Instruments and reagents

[0027] Agilent 1260 high performance liquid chromatography; Agilent C18 liquid chromatography column (150×4.6mm, 5μm); SQP electronic analytical balance from Sartorius, Germany; Millipore simplicity-185 ultrapure water device; Kunshan Ultrasonic Instrument Co., Ltd. KQ5200E ultrasonic Washer; L600 low-speed centrifuge; Hunan Xiangyi Laboratory Instrument Development Co., Ltd. TGL-20M desktop high-speed refrigerated centrifuge; DHG-9146A electric heating constant temperature blast drying oven; HWS-26 electric heating constant temperature water bath; acetonitrile (chromatographically pure, Sinopharm Group Chemical Reagent Co., Ltd.); Methanol (analytical grade, Sinopharm Group Chemical Reagent Co., Ltd.); ultrapure water; other reagents were analytically pure. There ...

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Abstract

The invention belongs to the technical field of chemistry, and specifically relates to a method for measuring the contents of multiple components of taxillus sutchuenensis. According to the method, under same chromatographic conditions, the contents of quercetin, quercitrin, and avicularin of taxillus sutchuenensis are measured by liquid chromatography at the same time. High performance liquid chromatography (HPLC) is used to simultaneously measure the contents of quercetin, quercitrin, and avicularin of taxillus sutchuenensis. The detection method is simple and accurate, the repeatability isgood, and reference is provided for comprehensive evaluation and control on the quality of taxillus sutchuenensis.

Description

【Technical field】 [0001] The invention relates to the technical field of chemistry, in particular to a multi-component content determination method of mulberry parasites. 【Background technique】 [0002] Sangji is the dry leafy stem branch of Taxillus chinensis (DC.) Danse. Harvest from winter to next spring, remove thick stems, cut into sections, dry, or steam and dry. The use of Sangjishen as medicine was first recorded in "Shen Nong's Materia Medica", named "Sangshangjishen", which was listed as top grade. "Famous Doctors Bielu" says: "A twig grows on the mulberry tree in Hongnongchuan Valley, and the stems and leaves are picked on March 3, and dried in the shade." On trees such as maple, the seeds are yellow and as big as jujubes. But there are mulberry trees in Guozhou, the seed juice is very sticky, and the core is as big as a small bean; the leaves have no yin and yang, and are thick like thin willow leaves; the late stems are thick and short. It is irrelevant to in...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 银胜高李永华陆海琳夏天李耀华李斌李红菊
Owner GUANGXI UNIV OF CHINESE MEDICINE
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