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Preparation method and application of electrochemical immunosensor based on Hemin-rGO (reduced graphene oxide)

An immunosensor and electrochemical technology, applied in the field of immune analysis and biosensing, can solve the problems of early detection and diagnosis of cancer, and achieve the effect of amplifying electrical response signals, simple operation, and efficient transmission

Inactive Publication Date: 2018-08-21
SHANDONG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The cure rate of early cancer patients can reach more than 80%, but there are certain difficulties in the early detection and diagnosis of cancer, and the detection of related cancer tumor markers is an important way to detect cancer early

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Embodiment 1 A preparation method of an electrochemical immunosensor based on Hemin-rGO, comprising the following steps:

[0046] (1) Polish a glassy carbon electrode with a diameter of 4mm to a mirror surface with alumina polishing powder, and clean it ultrasonically in absolute ethanol;

[0047] (2) Add 6.0 µL of Au NPs solution dropwise to the electrode surface, dry it at room temperature, wash the electrode surface with ultrapure water, and dry it;

[0048] (3) Continue to add 6.0 µL, 10 µg / mL tumor marker capture antibody Ab 1 Add it dropwise to the surface of the electrode, and place it in a refrigerator at 4.0 °C to dry;

[0049] (4) Add 3.0 µL, 0.5% bovine serum albumin BSA solution dropwise to the electrode surface to seal the non-specific active sites on the electrode surface, wash the electrode surface with pH=7.4 phosphate buffer, and place it at 4.0 ° C to dry in the refrigerator;

[0050] (5) Add 6.0 μL, 10fg / mL ~ 200 ng / mL of a series of tumor marker a...

Embodiment 2

[0052] Embodiment 2 A preparation method of an electrochemical immunosensor based on Hemin-rGO, comprising the following steps:

[0053] (1) Polish a glassy carbon electrode with a diameter of 4mm to a mirror surface with alumina polishing powder, and clean it ultrasonically in absolute ethanol;

[0054] (2) Add 6.0 µL of Au NPs solution dropwise to the electrode surface, dry it at room temperature, wash the electrode surface with ultrapure water, and dry it;

[0055] (3) Continue to add 6.0 µL, 15 µg / mL tumor marker capture antibody Ab 1 Add it dropwise to the surface of the electrode, and place it in a refrigerator at 4.0 °C to dry;

[0056] (4) Add 3.0 µL, 1.0% bovine serum albumin BSA solution dropwise to the electrode surface to seal the non-specific active sites on the electrode surface, wash the electrode surface with pH=7.4 phosphate buffer, and place it at 4.0 ° C to dry in the refrigerator;

[0057] (5) Add 6.0 μL, 10fg / mL ~ 200 ng / mL of a series of tumor marker a...

Embodiment 3

[0059] Embodiment 3 A preparation method of an electrochemical immunosensor based on Hemin-rGO, comprising the following steps:

[0060] (1) Polish a glassy carbon electrode with a diameter of 4mm to a mirror surface with alumina polishing powder, and clean it ultrasonically in absolute ethanol;

[0061] (2) Add 6.0 µL of Au NPs solution dropwise to the electrode surface, dry it at room temperature, wash the electrode surface with ultrapure water, and dry it;

[0062] (3) Continue to add 6.0 µL, 20 µg / mL tumor marker capture antibody Ab 1 Add it dropwise to the surface of the electrode, and place it in a refrigerator at 4.0 °C to dry;

[0063] (4) Add 3.0 µL, 1.5% bovine serum albumin BSA solution dropwise to the electrode surface to seal the non-specific active sites on the electrode surface, wash the electrode surface with pH=7.4 phosphate buffer, and place it at 4.0 ° C to dry in the refrigerator;

[0064] (5) Add 6.0 µL, 10 fg / mL ~ 200 ng / mL of a series of tumor marker ...

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Abstract

The invention belongs to the technical field of immune analysis and biosensing detection, and particularly relates to a preparation method and an application of an electrochemical immunosensor based on Hemin-rGO (reduced graphene oxide). A Hemin-rGO (reduced graphene oxide) porous composite material separated by microporous carbon spheres loaded with silver nanoparticles is used as a detection antibody marker, and the constructed sandwich electrochemical immunosensor realizes detection of tumor marker carcinoembryonic antigens, has the advantages of high sensitivity, high specificity, low cost, low detection limit and the like, and has important scientific significance and application value for early diagnosis and disease monitoring of lung cancer.

Description

technical field [0001] The invention belongs to the technical field of immune analysis and biosensing, and provides a preparation method and application of an electrochemical immunosensor based on Hemin-rGO, specifically a Hemin-rGO porous composite material separated by microporous carbon spheres loaded with silver nanoparticles. The material is used as a detection antibody marker to realize the sensitive determination of the tumor marker carcinoembryonic antigen. Background technique [0002] Cancer is a disease with high morbidity and high mortality that seriously threatens human health. According to the latest data from the World Health Organization (WHO) in February 2018, nearly one-sixth of the deaths are caused by cancer, and it is expected that the number of new cases will continue to increase in the next two decades. Cancer has become the main cause of human death. One of the killers. The cure rate of early cancer patients can reach more than 80%, but there are ce...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327G01N27/26
CPCG01N27/26G01N27/308G01N27/327
Inventor 李月云张春燕张栓贾翌雷王粤博陈志伟董云会
Owner SHANDONG UNIV OF TECH
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