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Haemophilus parasuis trivalent inactivated vaccine as well as production method and application thereof

A technology of Haemophilus suis and inactivated vaccines, which is applied in the field of Haemophilus parasuis trivalent inactivated vaccines, can solve the problems of low cross-protection rate and achieve high immune efficacy, long immune period and good safety

Inactive Publication Date: 2018-08-24
HENAN UNIV OF SCI & TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the domestic Haemophilus parasuis vaccine is only the bivalent (serum type 4 and 5) inactivated vaccine of Haemophilus parasuis, but various studies have shown that the cross-protection rate between the various types of Haemophilus parasuis is low

Method used

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  • Haemophilus parasuis trivalent inactivated vaccine as well as production method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] The screening and identification of embodiment 1 Haemophilus parasuis

[0034] 1. Materials and methods

[0035] 1.1 Source of disease material

[0036] From October 2008 to March 2010, a total of 137 pigs with suspected clinical symptoms of Haemophilus parasuis were collected from Henan, Shandong, Hubei, Anhui, Jiangsu, Hebei and other provinces. Affected pigs often showed fever, dyspnea, joint swelling, lameness, cyanosis of skin and mucous membranes, difficulty in standing or even paralysis, stiff pigs or death. At the same time as the lungs were collected, heart blood, lymph nodes, spleen, brain, joint fluid and other diseased tissues were collected from some pigs for the isolation and identification of bacteria.

[0037] 1.2 Medium

[0038] The preparation method of Tryptic Soy Agar (Tryptic Soy Agar, TSA) medium is as follows: Take 40g of Tryptic Soy Agar, add deionized water, dilute to 1000mL, shake well to dissolve, sterilize by high pressure steam at 121℃ fo...

Embodiment 2

[0073] The production method of embodiment 2 Haemophilus parasuis 4, 5 and 12 type trivalent inactivated vaccines

[0074] 1. Seed Preparation for Production

[0075] (1) First-class seed propagation and identification

[0076] The production strains of Haemophilus parasuis type 4 H4L1 strain, type 5 H5L3 strain and type 12 H12L3 strain were respectively inoculated on TSA plates containing newborn bovine serum (final concentration 10%) and NAD (final concentration 100 μg / mL), Cultivate at 37°C for 24-48 hours, select more than 5 typical colonies for each strain, and inoculate several branches of TSA slant containing newborn bovine serum (final concentration 10%) and NAD (final concentration 100 μg / mL), and culture at 37°C for 24-48 hours. Hours, after passing the pure inspection, it will be regarded as the first-class seed. Stored at 2-8°C, it should not exceed 7 days, and the subculture should not exceed 5 generations.

[0077] (2) Secondary seed propagation

[0078] The ...

Embodiment 3

[0098] Example 3 Haemophilus parasuis 4, 5 and 12 type trivalent inactivated vaccine finished product inspection

[0099] (1) traits

[0100] This product is a gray-white suspension, with a small amount of sediment at the bottom after standing for a long time, and it becomes a homogeneous suspension after shaking.

[0101] (2) Loading inspection

[0102] Tested according to the appendix of the current "Chinese Veterinary Pharmacopoeia", it should meet the requirements.

[0103] (3) Sterility test

[0104] Tested according to the appendix of the current "Chinese Veterinary Pharmacopoeia", it should grow aseptically.

[0105] (4) Safety inspection

[0106] Use 5 healthy susceptible piglets (see Note 4) aged 3 to 4 weeks, inject 4 mL of vaccine into each neck muscle, and observe for 14 days. There should be no local or systemic adverse reactions caused by the vaccine.

[0107] (5) Efficacy test Choose one of the following methods.

[0108] ① Guinea pig immune antibody assay...

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Abstract

The invention discloses a haemophilus parasuis trivalent inactivated vaccine as well as a production method and application thereof. The haemophilus parasuis trivalent inactivated vaccine contains a haemophilus parasuis type-4 H4L1 strain, a type-5 H5L3 strain and a type-12 H12L3 strain being inactivated by a formaldehyde solution, as well as a water-based immunologic adjuvant, wherein the haemophilus parasuis type-4 H4L1 strain, the type-5 H5L3 strain and the type-12 H12L3 strain are all collected in the China Center for Type Culture Collection on 11 January, 2018 with the collection numbersof CCTCC M 2018019, CCTCC M 2018020 and CCTCC M 2018021 respectively. The trivalent inactivated vaccine disclosed by the invention is used for preventing the haemophilus parasuis disease caused by thetype-4, type-5 and type-12 haemophilus parasuis, and has the advantages of high security, high immune efficacy, and long immunity period and the like.

Description

technical field [0001] The invention relates to the technical field of veterinary biological products, in particular to a trivalent inactivated vaccine of Haemophilus parasuis and its production method and application. Background technique [0002] Haemophilus parasuis disease, also known as Glaesser's disease, is a disease caused by Haemophilus parasuis with respiratory symptoms, lameness, pleurisy, pericarditis, peritonitis, arthritis and meningitis. Characteristic porcine respiratory infectious disease. With the development of the pig industry, Haemophilus parasuis has become a new bacterial infectious disease that seriously endangers the pig industry in recent years. It is distributed worldwide and has been confirmed to exist and prevail in most pig farms in my country. It can affect pigs aged 2 weeks to 4 months, but it mainly occurs around weaning and in piglets. It is common in pigs aged 5 to 8 weeks. The incidence is generally 10 to 15%, and the mortality rate can re...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A61K39/116A61K39/102A61P31/04C12R1/21
CPCA61K39/102A61K2039/521A61K2039/552A61K2039/70A61P31/04C12N1/20C12N1/205C12R2001/21
Inventor 赵战勤喻正军巢伟黄岚芬
Owner HENAN UNIV OF SCI & TECH
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