Method for screening medium-trace neuraminidase inhibitors in honeysuckle flower

A technology of neuraminidase and screening method is applied in the field of separation of Chinese herbal medicine compounds to achieve good screening effect, enrichment and superior technical effect.

Active Publication Date: 2018-08-24
BEIJING DAWN AEROSPACE BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the limitation of the sensitivity of the method, there are certain limitations in the screening of trace active ingredients in complex matrices

Method used

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  • Method for screening medium-trace neuraminidase inhibitors in honeysuckle flower
  • Method for screening medium-trace neuraminidase inhibitors in honeysuckle flower
  • Method for screening medium-trace neuraminidase inhibitors in honeysuckle flower

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Experimental program
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Embodiment 1

[0056] 1. Sample pretreatment

[0057] Put 0.5g of honeysuckle powder and 50mL of extraction solvent into a stoppered Erlenmeyer flask, place it in an ultrasonic chamber for ultrasonic extraction at 25°C for 30min, and filter the extract with suction, then evaporate the filtrate to dryness under reduced pressure. The residue was redissolved by adding 2 mL of 50% (v / v) methanol solution, passed through a 0.22 μm microporous membrane, and stored at 4° C. for use. The four extraction solutions are water, 80% ethanol, ethyl acetate and petroleum ether.

[0058] Solution preparation: Buffer 1 consists of 33mmol / L MES and 4mmol / L CaCl 2 made of ultrapure water with a pH of 6.5. Buffer 2 consists of 33mmol / L MES, 4mmol / L CaCl 2 and 10% isopropanol in ultrapure water with a pH of 6.5. Accurately weigh 1 mg of apigenin standard substance, and dilute to 1 mg / mL apigenin standard solution in a volumetric flask as a positive control.

[0059] 2. Components separated by semi-prep-HPLC...

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Abstract

The invention provides a 2D-HPLC-AS/UF and ESI-TOF/MS method for screening and characterizing medium-trace neuraminidase inhibitors in honeysuckle flower. By adoption of the method, 44 neuraminidase inhibitory active compounds are found from a honeysuckle flower extract and are primarily identified by comparison with standards and references. The method has the advantages of high efficiency, low energy consumption and high pertinence, and provides the method and technical support for rapid screening and identification of medium-trace neuraminidase inhibitors in complex natural medicine materials.

Description

technical field [0001] The invention relates to the field of separation of Chinese herbal medicine compounds, in particular to a method for screening and characterizing trace neuraminidase inhibitors in Flos Lonicerae by combining 2D-HPLC-AS / UF and ESI-TOF / MS. Background technique [0002] Influenza is an acute respiratory infection caused by influenza virus. It is highly contagious, has a high incidence rate, and is easy to cause outbreaks or pandemics. The target of anti-influenza virus drugs is the virus's own structure, enzymes and replication mechanism, so when the active ingredients of anti-influenza virus drugs attack the virus, they often have adverse reactions to the host cells. Therefore, the research and development of anti-influenza virus drugs is very difficult. At present, the main way to prevent influenza is to inoculate influenza vaccine, but the gene mutation rate of influenza virus is high, and the existing anti-influenza vaccines are often ineffective aga...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/72G01N30/46G01N30/06G01N30/12G01N30/14G01N30/08
CPCG01N30/02G01N30/06G01N30/08G01N30/12G01N30/14G01N30/46G01N30/72G01N2030/065G01N2030/126G01N2030/146
Inventor 赵恒强张敏敏王晓崔莉闫慧娇耿岩玲
Owner BEIJING DAWN AEROSPACE BIO TECH
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