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Preparation method and application of sericin and gamma-polyglutamic acid self-assembled nano-micelle

A technology of sericin and polyglutamic acid, which is applied in the research and preparation of drug carriers, can solve the problems of sericin and acrylic acid toxicity, and achieve the effect of broad application prospects

Active Publication Date: 2018-09-04
ZHONGYUAN ENGINEERING COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved by the present invention is the toxic operation process of the polymerization reaction of sericin and acrylic acid (AA). At present, there is no research on self-assembly between sericin and other proteins or polymers, and a non-toxic Preparation method and application of pollution-free sericin and γ-polyglutamic acid self-assembled nanomicelle

Method used

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  • Preparation method and application of sericin and gamma-polyglutamic acid self-assembled nano-micelle

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Embodiment 1

[0021] A preparation method of sericin and γ-polyglutamic acid self-assembled nano-micelle, comprising the following steps:

[0022] 1) Selection of sericin and γ-polyglutamic acid

[0023] The molecular weight of silk fibroin is selected as 12000-24000Da, and the molecular weight of gamma-polyglutamic acid is selected as 10ku.

[0024] 2) Preparation of sericin and γ-polyglutamic acid solution

[0025] A certain mass of sericin and γ-polyglutamic acid were dissolved in deionized water respectively to prepare an aqueous solution with a mass concentration of 0.3% for use.

[0026] 3) Preparation of sericin and γ-polyglutamic acid self-assembly

[0027] Mix sericin and γ-polyglutamic acid solutions with a mass concentration of 0.3% in a mass ratio of 4:1, and add 2 times the volume of phthalic acid-hydrochloric acid buffer solution (pH2.2-3.8) (0.05 mol / L), use 0.01 mol / L hydrochloric acid to fine-tune the pH value within this range to 3.5, and put it into an ultrasonic devic...

Embodiment 2

[0030] A preparation method of sericin and γ-polyglutamic acid self-assembled nano-micelle, comprising the following steps:

[0031] 1) Selection of sericin and γ-polyglutamic acid

[0032] The molecular weight of sericin is 12000-24000Da, and the molecular weight of γ-polyglutamic acid is 70ku.

[0033] 2) Preparation of sericin and γ-polyglutamic acid solution

[0034] A certain mass of sericin and γ-polyglutamic acid were dissolved in deionized water to prepare a solution with a mass concentration of 0.9% for later use.

[0035] 3) Preparation of sericin and γ-polyglutamic acid self-assembly

[0036] Preparation process: Mix sericin and γ-polyglutamic acid solutions with the same mass concentration of 0.9% and a mass ratio of 2:1, add 3 times the volume of phthalic acid-hydrochloric acid buffer solution (pH2.2-3.8 ) (0.05mol / L), use 0.01 mol / L to fine-tune the pH value to 3.0, put it into an ultrasonic device for ultrasonication for 2 hours, power 300W, frequency 40KHZ. ...

Embodiment 3

[0039] A preparation method of sericin and γ-polyglutamic acid self-assembled nano-micelle, comprising the following steps:

[0040] 1) Selection of sericin and γ-polyglutamic acid

[0041] The molecular weight of sericin is 12000-24000Da, and the molecular weight of γ-polyglutamic acid is 120ku.

[0042] 2) Preparation of sericin and γ-polyglutamic acid solution

[0043] The preparation and application environment of self-assembled micelles should be carried out in an acidic environment (pH ≤ 7). Dissolve a certain amount of sericin and γ-polyglutamic acid in deionized water to prepare a solution with a mass concentration of 1.2% for later use. .

[0044] 3) Preparation of sericin and γ-polyglutamic acid self-assembly

[0045] Mix sericin and γ-polyglutamic acid solutions with a mass concentration of 1.2% and a mass ratio of 1:1, and add 3 times the volume of phthalic acid-hydrochloric acid buffer solution (pH2.2-3.8) (0.05mol / L), use 0.01 mol / L hydrochloric acid to fine...

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Abstract

The invention discloses a preparation method of sericin and gamma-polyglutamic acid self-assembled nano-micelle, which aims at solving the technical problem of no research on the self-assembling between the sericin and other proteins or polymers. The preparation method comprises the following steps: (1) selecting sericin with a molecular weight of 12,000 to 24,000Da and gamma-polyglutamic acid with the molecular weight of 10 to 120ku; (2) preparing a sericin solution and a gamma-polyglutamic acid solution having the same mass concentration: separately dissolving the sericin and gamma-polyglutamic acid in the deionized water, and preparing solutions having the same mass concentration in a range of 0.3 to 2 percent; and (3) preparing a sericin and gamma-polyglutamic acid self-assembled body.The gamma-polyglutamic acid and sericin nano self-assembled body is prepared by utilizing gamma-polyglutamic acid and sericin by virtue of an electrostatic assembling method for first time and can beused for the embedding of anticancer drugs and hydrophobic drugs.

Description

technical field [0001] The invention relates to the field of research and preparation of drug carriers, in particular to a self-assembled nano drug of sericin and γ-polyglutamic acid whose raw materials are relatively high molecular weight sericin and γ-polyglutamic acid Preparation method and use of slow-release micelles. Background technique [0002] Sericin is a natural macromolecular protein wrapped on the surface of silk fiber. It is the main protein that constitutes silk. In the textile industry, sericin is discarded as a by-product. There are about 50,000 tons of sericin every year. Treated as waste, wasting a lot of natural protein resources. Sericin has good biological activity, anti-tumor activity and cell adhesion, which has laid a foundation for its application in the field of biomedicine and health care products. Some people have prepared sericin and acrylic acid (AA) through polymerization. A novel mucoadhesive polymer, but this polymerization method is a tox...

Claims

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Application Information

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IPC IPC(8): A61K9/107A61K47/34A61K47/42A61K31/12A61K31/015A61P35/00
CPCA61K9/1075A61K31/015A61K31/12A61K47/42A61P35/00
Inventor 苏小舟栗蕾陈劲松
Owner ZHONGYUAN ENGINEERING COLLEGE