Preparation method of bamboo bird's nest mannan

Abstract

The invention relates to a preparation method of bamboo bird's nest mannan. The preparation method comprises the following steps of a, raw material enzymolysis: crushing bamboo bird's nest; then, adding 10 to 30 times of quantity of water for soaking so that powder fully absorbs water for expansion; the pH value is regulated to be 5.5 to 6.5; adding cellulase according to the standard that 1100 to4500 thousand U of cellulase is added into per kg of bamboo bird's nest; performing enzymolysis for 1 to 4h at 35 to 40 DEG C to obtain an enzymolysis solution; b, hot water extraction and concentration: adding 2 to 6kg of hot water into per kg of enzymolysis solution; performing extraction for 1 to 3h at the temperature of 90 to 100 DEG C; then, lowering the temperature to 35 to 45 DEG C; performing extraction treatment; c, ethanol precipitation: after the enzymolysis solution is subjected to concentration treatment, adding ethyl alcohol, so that the ethyl alcohol concentration reaches 40 to80 percent; the pH value is regulated to be 9 to 10; performing ultrasonic treatment at the frequency being 20 to 100KHz at 40 to 80 DEG C; separating out insoluble substances after the ultrasonic treatment for 2 to 4h; d, removing protein by an ultrafiltration method, and obtaining the bamboo bird's nest mannan after the drying. The preparation method has the characteristics that the preparationprocess is simple; the active polysaccharide mannan can be obtained from the bamboo bird's nest through separation, so that the immunoregulatory activity is developed, and the like.

Description

technical field [0001] The invention relates to a preparation method of bamboo bird's nest mannan with immune regulation function, and belongs to the technical field of biomedicine. Background technique [0002] Bamboo bird's nest (English name: Bamboo bird's nest), also known as bamboo fungus, bamboo flower, bamboo mushroom, bamboo mat, is a very famous and precious fungal food. [0003] Bamboo bird's nest is a special kind of young bamboo parasites that eat bamboo juice. The remaining bamboo juice is a fungus that grows at a suitable temperature and water. At the same time, it absorbs the bamboo juice and the nutrition of the bamboo insects. Its shape and taste are like bird's nest, with smooth and refreshing taste, which is fresh, tender, crisp and refreshing. Its nutritional value is comparable to bird's nest, so it is called plant bird's nest. Bamboo bird's nest mainly contains protein, fat and 6 kinds of amino acids necessary for the human body, and also contains vita...

AI Technical Summary

Problems solved by technology

Because bamboo bird's nest cannot be planted artificially, and it only grows in mid-autumn, it is very valuable. However, there are few publications about the utilization and artificial cultivation of bamboo bird's nest.