Molecular marker correlated to characteristics of maroon feathers of quails and application thereof

A molecular marker, quail technology, applied in the determination/inspection of microorganisms, DNA/RNA fragments, recombinant DNA technology, etc., can solve the problem of lack of identification of homozygous genotypes and heterozygous genotypes in feather color, and save breeding costs. , The effect of short time and easy operation

Active Publication Date: 2018-09-11
INST OF ANIMAL SCI & VETERINARY HUBEI ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved by the present invention is to provide a molecular marker related to the heterozygous type of chestnut plumage in order

Method used

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  • Molecular marker correlated to characteristics of maroon feathers of quails and application thereof
  • Molecular marker correlated to characteristics of maroon feathers of quails and application thereof
  • Molecular marker correlated to characteristics of maroon feathers of quails and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1 Obtaining of Quail CCDC171 Gene Fragment and Establishment of Mutation Detection Method

[0023] 1. Extraction of quail genomic DNA

[0024] The experimental quail of the present invention is "Shendan No. 1" egg quail with yellow feather and chestnut feather male quail (the sample comes from the breeding quail farm of Hubei Shendan Health Food Co., Ltd.). Quail genomic DNA was extracted using a blood genomic DNA extraction kit (operated in accordance with the kit instructions) produced by Beijing Quanshijin Biotechnology Co., Ltd. The specific steps are as follows:

[0025] (1) Take 10 μL of quail anticoagulated blood, add 20 μL of proteinase K, 500 μL of BB3, vortex for 15 s to fully mix the sample, and incubate at room temperature for 10 min;

[0026] (2) Briefly centrifuge, transfer the entire solution into a spin column, centrifuge at 12,000×g for 1 min, and discard the filtrate;

[0027] (3) Add 500 μL solution CB3, centrifuge at 12000×g for 30 s, and d...

Embodiment 2

[0052] Example 2 The application of the molecular marker prepared by the present invention in the selection of sorrel feather heterozygous male quail

[0053] Using the molecular markers prepared by the present invention, the applicant detected the distribution frequencies of the two alleles of the quail CCDC171 gene in two populations of male quails with yellow feathers and sorrel feathers. The detection results are shown in Table 1.

[0054] Table 1 Distribution of alleles at mutation site 210 of CCDC171 gene in different plumage color male quail populations

[0055]

[0056] From the results in Table 1, it can be seen that the frequency of the A allele is 100% in male yellow-feathered quails, indicating that individuals with the AA genotype are all yellow-feathered and homozygous; The frequency of the allele is 90%, and the frequency of the A allele is 10%, indicating that in the sorrel male quail population, some individuals have a heterozygous AG genotype, and most ind...

Embodiment 3

[0057] Example 3 Application of the molecular markers prepared by the present invention in quail self-sexing mating lines

[0058] From the results in Example 2, it can be seen that the genotypes of male sorrel plumage quail are GG and GA, and the GA type is heterozygous. In this example, the above-mentioned genotypes are heterozygous individuals by the method of molecular marker-assisted selection. Selected to form a new group of sorrel feather male quail, breed offspring by crossing with yellow feather female quail, and observe the feather color and sex of the offspring.

[0059] After molecular marker-assisted selection, 11 heterozygous individuals whose sorrel feather genotype was GA were selected, and 132 yellow feather female quails were selected to form 11 families (each family had 1 sorrel feather male quail and 12 yellow feather quails). feather female quail) to breed offspring, the specific process is as follows:

[0060] 1. Breeding and egg collection

[0061] The...

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Abstract

The invention discloses a molecular marker correlated to characteristics of maroon feathers of quails and application thereof, which belongs to the technical field of poultry molecular marker (geneticmarker) preparation and application. An A/G base mutation existed on a 10-393 bp sequence of an N terminal of a quail CCDC171 gene is adopted as the genetic marker correlated to the maroon feathers of the quails, and the molecular marker can be used for screening out hybrid individuals in maroon male quails. The invention also discloses a preparation method of the genetic marker, and applicationthereof in quail maroon feather hybrid subtype male quail screening and quail auto sexing matching screening. According to the method, the accuracy rate reaches to 99.6 percent or more, and the male quails eliminated in an inbred strain and female quails for laying eggs can be fully utilized as seeds, so that the utilization ratio of seed quails of the breeding enterprise is greatly improved, andthe breeding cost of the breeding enterprise is saved.

Description

technical field [0001] The invention belongs to the technical field of preparation and application of poultry molecular markers (genetic markers), in particular to a molecular marker related to quail chestnut feathers and its application. The genetic marker is cloned from the quail CCDC171 gene. Background technique [0002] Quail is a special economic poultry with high edible value and therapeutic effect. Quail eggs are rich in nutrition, high in protein and low in cholesterol. With the continuous improvement of people's living standards, quail eggs are more and more favored by consumers. Quail feather color is sex-linked inheritance, which is regulated by a gene located on sex chromosome Z. Studies have shown that yellow feathers are recessive to chestnut feathers, and follow the recessive sex-linked inheritance rule (Pang Youzhi et al. The genetic basis of the production of male and female mating lines [J]. China Poultry, 2001, 23 (6): 37-38), in the conventional breedi...

Claims

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Application Information

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IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156
Inventor 吴艳皮劲松杜金平潘爱銮申杰蒲跃进张昊梁振华孙静
Owner INST OF ANIMAL SCI & VETERINARY HUBEI ACADEMY OF AGRI SCI
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