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Mutation breeding method of buckwheat

A technology for mutation breeding and buckwheat is applied in the directions of botanical equipment and methods, applications, and plant genetic improvement, to achieve the effects of shortening breeding time, increasing mutation rate, and quickly stabilizing traits.

Inactive Publication Date: 2018-09-14
QINGYANG DUNBO TECH DEV CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, the mutation breeding of buckwheat is only a kind of physical mutagenesis or chemical mutagenesis alone, and there is no combination of the two methods to quickly improve the breeding efficiency of buckwheat

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] 1) Select seeds: select seeds that are plump, mildew-free, pest-free, and non-damaged in the current season, wash the selected seeds with clean water 3 times, remove the dust on the surface of the seeds, and place the seeds in 10% H 2 o 2 Take it out after soaking for 3 hours, rinse it with sterile water for 4 times, take it out and dry it naturally for later use;

[0021] 2) Physical mutagenesis: using 60 Co-γ-ray irradiation treatment, buckwheat seeds are packed into plastic bags for irradiation treatment, the radiation dose rate is 1Gy / min, and the radiation dose is 10Gy;

[0022] 3) Sowing: the above-mentioned radiation-treated seeds were planted in a modified MS medium, and placed in an artificial climate box at 25° C. for 15-20 days;

[0023] 4) Chemical induction: the above-mentioned buckwheat seedlings were moved into a nutrient pot, and after the seedlings grew to the branching stage, a chemical inducer was injected into the buckwheat stems every day, and the...

Embodiment 2

[0027] 1) Select seeds: select seeds that are plump, mildew-free, pest-free, and non-damaged in the current season, wash the selected seeds with clean water 3 times, remove the dust on the surface of the seeds, and place the seeds in 10% H 2 o 2 Take it out after soaking for 2 hours, rinse it with sterile water for 3 times, take it out and dry it naturally for later use;

[0028] 2) Physical mutagenesis: using 60 Co-γ-ray irradiation treatment, buckwheat seeds are packed into plastic bags for irradiation treatment, the radiation dose rate is 1Gy / min, and the radiation dose is 50Gy;

[0029] 3) Sowing: the above-mentioned radiation-treated seeds were planted in the improved MS medium, and placed in an artificial climate box at 25° C. for 20 days;

[0030] 4) Chemical induction: the above-mentioned buckwheat seedlings were moved into a nutrient pot, and after the seedlings grew to the branching stage, a chemical inducer was injected into the buckwheat stems every day for conti...

Embodiment 3

[0034] 1) Select seeds: select seeds that are plump, mildew-free, pest-free, and non-damaged in the current season, wash the selected seeds with clean water 3 times, remove the dust on the surface of the seeds, and place the seeds in 10% H 2 o 2 Take it out after soaking for 3 hours, rinse it with sterile water for 4 times, take it out and dry it naturally for later use;

[0035] 2) Physical mutagenesis: using 60 Co-γ-ray irradiation treatment, buckwheat seeds are packed into plastic bags for irradiation treatment, the radiation dose rate is 1Gy / min, and the radiation dose is 100Gy;

[0036] 3) Sowing: the above-mentioned radiation-treated seeds were planted in the improved MS medium, and placed in an artificial climate box at 28° C. for 20 days;

[0037] 4) Chemical induction: the above-mentioned buckwheat seedlings were moved into a nutrient pot, and after the seedlings grew to the branching stage, a chemical inducer was injected into the buckwheat stems every day, continu...

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Abstract

The invention provides a mutation breeding method of buckwheat, and belongs to the field of buckwheat breeding. The mutation breeding method comprises the following steps: 1), selecting seeds; 2), performing physical mutation: 3), sowing; 4), performing chemical induction; 5), diversion harvesting; and 6), screening mutants. The mutation breeding method has the beneficial effects as follows: by acombined breeding method of the physical mutation and the chemical induction, the mutation rate of buckwheat plants is greatly increased, the breeding time is shortened and traits are quickly stabilized.

Description

technical field [0001] The invention belongs to the field of buckwheat breeding, in particular to a buckwheat mutation breeding method. Background technique [0002] Buckwheat belongs to the genus Buckwheat of Polygonaceae. Although it is a minor crop in the production of food crops, it has a long history of planting and is planted all over the country. China's planting area and output rank second in the world because of its short growth period and adaptability. It has a special status in the production layout of crops, and it can be used not only as a main food crop, but also as a supplementary sowing crop and a disaster relief crop. It is called by nutritionists the most promising green food in the 21st century. The whole body is a treasure, with high nutritional value and medicinal value. It contains many bioflavonoid rutin that other crops do not contain, and is beneficial to human health. Flavonoids have antioxidant properties, inhibit lipid peroxidation, maintain vaso...

Claims

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Application Information

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IPC IPC(8): A01H1/06
CPCA01H1/06
Inventor 房世平秦国鹏胡丹
Owner QINGYANG DUNBO TECH DEV CO LTD
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