DNA sensor, preparation method thereof, and method for detecting short chain DNA

A sensor and short-chain technology, applied in the field of biosensors, can solve the problems of low sensitivity, complexity, and long measurement time, and achieve the effects of wide detection range, low detection limit, and good selectivity

Active Publication Date: 2018-10-02
青岛言鼎生物医疗科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Its disadvantages include relatively long assay times, high assay costs, and error-prone nature that occasionally leads to "false positive" signals
FISH technology requires a complex fluorescent coloring system, its sensitivity is still not high, and it cannot be widely used clinically

Method used

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  • DNA sensor, preparation method thereof, and method for detecting short chain DNA
  • DNA sensor, preparation method thereof, and method for detecting short chain DNA
  • DNA sensor, preparation method thereof, and method for detecting short chain DNA

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] A method for detecting short-chain DNA, detecting short-chain DNA species of chronic myeloid leukemia, comprising the following steps:

[0043] 1) Preparation of polypyrrole / silver@silver chloride composite electrode: prepared by the method disclosed in 201410299007.8.

[0044]2) Preparation of DNA sensor: immerse the polypyrrole / silver@silver chloride composite electrode prepared in step 1) into 50 μL of 4 μM hairpin DNA HP1 solution and incubate at 4°C for 12 hours, take out the electrode, and wash , after drying, put the electrode into 50 μL of different concentrations of type b3a2 DNA solution for incubation, the incubation temperature is 37 ° C, and the incubation time is 2 hours, the electrode is taken out, washed and dried, and then the electrode is placed in 50 μL of 1 μM 8 -17DNAzyme and 1 μL of 20 μM Zn 2+ The solution was incubated in the mixed solution, the incubation temperature was 37° C., and the incubation time was 90 minutes. The electrode was taken ou...

Embodiment 2

[0055] Repeat Example 1, and replace the short-chain DNA with a short-chain DNA with one base difference, a short-chain DNA with three base differences, a completely different short-chain DNA and a blank sample at different positions of the target DNA, The result is as Figure 4 .

Embodiment 3

[0057] Repeat Example 1, change the concentration of 8-17zyme in the detection process, and obtain the results such as Figure 5 .

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Abstract

The invention provides a DNA sensor, a preparation method thereof, and a method for detecting short chain DNA. The DNA sensor preparation method comprises following steps: soaking a prepared polypyrrole / silver @ silver chloride composite material electrode in a hairpin DNA HP1 solution; after incubation, dipping the electrode into short chain DNA with different concentrations to carry out incubation, and then soaking the electrode into a 8-17 DNAzyme solution containing Zn<2+> to prepare the DNA sensor. Gallic acid is taken as an electric active substance, and by detecting the responding strength of light current, the short chain DNA concentration can be detected quantitatively. Compared with the prior art, the provided PEC DNA detection method has the advantages of low detection limit andwide detection range, has good selectivity on target DNA, and can be widely used to detect various DNA sequences, in particular, specific short DNA sequences. A novel method is provided for detectingtrace DNA sequences. An effective detection method is provided for the early diagnosis of various diseases.

Description

technical field [0001] The invention belongs to the technical field of biosensors, and specifically designs a DNA sensor and its preparation method, a method for detecting short-chain DNA, and uses a polypyrrole / silver@silver chloride nanowire composite electrode as an electrode material to photoelectrically detect chronic myeloid leukemia short-chain DNA species. Background technique [0002] Chronic myelogenous leukemia (CML) is a clonal myeloproliferative disorder. Patients with chronic myelogenous leukemia do not have any obvious symptoms at the initial stage, and the chronic disease course can last for 3-5 years. These phenomena bring difficulties to the diagnosis of CML. The BCR / ABL gene is the traditional gene of the disease and is present in almost all cases of CML patients. [0003] There are many types of BCR / ABL gene, and b3a2 type is one of the most common mutation types. Therefore, the detection of b3a2 gene will be helpful for early diagnosis and monitoring ...

Claims

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Application Information

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IPC IPC(8): G01N21/27G01N27/327C12Q1/6825
CPCC12Q1/6825G01N21/27G01N27/3278
Inventor 张小俊刘翠婷
Owner 青岛言鼎生物医疗科技有限公司
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