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Sample crystal particle detection system and method

A particle detection and crystal technology, applied in measuring devices, instruments, fluorescence/phosphorescence, etc., to achieve uniform size, avoid light energy loss, and stable and reliable signals

Active Publication Date: 2018-10-02
MEDCAPTAIN MEDICAL TECH
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  • Abstract
  • Description
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Problems solved by technology

[0004] In addition, due to the inhibitory effect of PCR amplification products on enzyme-catalyzed reactions, the current gene variation detection method of qPCR technology is often powerless for low-abundance tumor gene variation in somatic cells

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  • Sample crystal particle detection system and method
  • Sample crystal particle detection system and method
  • Sample crystal particle detection system and method

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Embodiment Construction

[0051] The present invention will be further described below in conjunction with the accompanying drawings and specific embodiments.

[0052] Such as figure 1 , figure 2 The illustrated embodiment is a sample crystal particle detection system, including a controller, 8 sample crystal particle generators 11, a constant current and constant pressure device 302, 8 heat conduction containers 15 filled with crystal particle carrier oil, and 1 The crystal particle collection device 303 connected to the bottom of the heat conduction container, the crystal particle optical path detector 306, the six lasers 304 with successively increasing wavelengths arranged on one side of the crystal particle optical path detector, and the excitation light provided at the light outlet of each laser The filter assembly 305, the receiving light filter assembly 307 located on the other side of the crystal particle optical path detector, the photoelectric signal collector 308 connected to the receivin...

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Abstract

The invention discloses a sample crystal particle detection system and method. The system comprises a controller, M sample crystal particle generators, a constant current constant voltage device, M heat-conducting containers filled with crystal particle carrier oil, a crystal particle collection device connected with the bottom of one heat-conducting container, a crystal particle light path detector, a plurality of lasers of which wavelengths are sequentially increased, exciting light filter assemblies, a received light filter assembly, a photoelectric signal collector, a PC and a temperaturecontrol device, wherein the lasers are positioned on one side of the crystal particle light path detector and arranged in sequence; the exciting light filter assemblies are arranged at light outlets of the various lasers; the received light filter assembly is positioned on the other side of the crystal particle light path detector; the photoelectric signal collector is connected with the receivedlight filter assembly. The sample crystal particle detection system disclosed by the invention has the characteristics that an optical crosstalk phenomenon does not occur between light paths of the wave bands, the top-pressing and bottom-arranging structure is adopted, and dead space does not remain in the chip.

Description

technical field [0001] The invention relates to the technical field of gene molecular diagnostic reagent equipment, in particular to a sample crystal particle detection system and method with excellent stability and reproducibility, ultra-high sensitivity, and extreme accuracy. Background technique [0002] At present, fluorescence quantitative PCR (Fluorescence Quantitative Polymerase Chain Reaction, qPCR) has developed into a key routine technology in the field of in vitro molecular diagnosis, which has greatly promoted the development of life science in the field of health care. However, fluorescence quantitative PCR quantification is only relative quantification, and its accuracy and reproducibility still cannot meet the development needs of the current field of in vitro molecular diagnostic systems and biological genetics research. However, in many projects such as liquid biopsy and NIPT, there are some technical bottlenecks. For example, the content of tumor circulatin...

Claims

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Application Information

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IPC IPC(8): G01N21/64
CPCG01N21/6402G01N21/6486
Inventor 王亚琴
Owner MEDCAPTAIN MEDICAL TECH
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