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Extraction process for phycocyanin, chlorophyll and spirulina polysaccharides in fresh spirulina

A technology of spirulina polysaccharides and phycocyanin, which is applied in chemical instruments and methods, decapeptides, organic chemistry, etc., can solve the problems of waste of resources, inability to fully and effectively utilize spirulina resources, single active substances, etc., and achieve simple process , to achieve the effect of comprehensive utilization and in-depth development

Inactive Publication Date: 2018-10-09
江苏海睿生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although there are some reports on the extraction of active substances from spirulina, when they are extracted and processed, they are often only aimed at a certain active substance, and cannot take into account the utilization of multiple biologically active substances, so that the existing ones cannot be fully and effectively utilized. Spirulina resources, causing waste of resources

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Centrifuge and wash 6kg of fresh spirulina once, weigh 2kg of algae mud, add 2L of water, stir evenly, freeze and thaw twice, freeze overnight at minus 20°C, dissolve at room temperature, and add 6L of water after the second dissolution, Leave it for 3 hours and centrifuge to obtain supernatant a and solid b. Adjust the pH of supernatant a to 4.2-4.5 with 6 mol / L hydrochloric acid, stir for half an hour and centrifuge at 4000 rpm for 15 minutes. Ultrafiltration once through a 6k filter membrane, followed by ultrafiltration once with 4 times distilled water, adjusted to pH 7.0, and then freeze-dried to obtain phycocyanin powder;

[0015] The solid b was extracted for 8 hours by ethanol Soxhlet extraction. The specific steps of the ethanol Soxhlet extraction were to add excess zinc sulfate in 90% ethanol, adjust the pH of hydrochloric acid to 2-3, and adjust the supernatant to 10% NaOH solution. pH 7-8, filter to remove insoluble impurities, continue to adjust the pH of t...

Embodiment 2

[0018] Centrifuge and wash 10kg of fresh spirulina once, weigh 4kg of algae mud, add 4L of water, stir evenly, freeze and thaw twice, freeze overnight at minus 20°C, dissolve at room temperature, and add 12L of water after the second dissolution, Place it for 3 hours and centrifuge to obtain supernatant a and solid b. Adjust the pH of supernatant a to 4.2-4.5 with 5 mol / L hydrochloric acid, stir for half an hour and then centrifuge. The centrifugation condition is to centrifuge at 4000 rpm for 15 minutes. The supernatant was ultrafiltered once through a 6k filter membrane, then ultrafiltered once with 4 times distilled water, adjusted to pH 7.0, and then freeze-dried to obtain phycocyanin powder;

[0019] The solid b was extracted for 10 hours by ethanol Soxhlet extraction. The specific steps of the ethanol Soxhlet extraction were 90% ethanol, adding excess zinc sulfate, and hydrochloric acid to adjust the pH to 2-3. The supernatant was washed with 10% NaOH solution Adjust the...

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PUM

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Abstract

The invention discloses an extraction process for phycocyanin, chlorophyll and spirulina polysaccharides in fresh spirulina and relates to the technical field of extraction processes for active substances in spirulina. The extraction process comprises the following steps: weighing fresh spirulina, then carrying out centrifugal washing for 1-3 times, weighing a spirulina paste, adding water which is 0.5-4 times of the weight of the spirulina paste, uniformly stirring, carrying out freeze thawing twice, freezing over night at minus 20 DEG C, dissolving at a room temperature, after secondary dissolution, adding water which is 2-8 times of the weight of the spirulina paste, placing for 2-8 hours, centrifuging so as to obtain supernate a and a solid b, extracting the solid b in an ethanol soxhlet extraction mode so as to obtain an extract and a solid c, and extracting phycocyanin from the supernate a; extracting a chlorophyll zinc sodium salt from the extract of the solid b; extracting spirulina polysaccharides from the solid c. The extraction process is simple, conventional spirulina resources can be sufficiently and effectively utilized, comprehensive utilization and deep developmentof the spirulina are achieved, and a great significance on development of the spirulina industry can be achieved.

Description

technical field [0001] The invention relates to an extraction process of phycocyanin, chlorophyll and spirulina polysaccharide in fresh spirulina, and belongs to the technical field of extraction process of active substances in spirulina. Background technique [0002] Spirulina is Cyanophyta, Cyanophyceae, Cyanophyceae, and Spirulina is a lower aquatic plant in Oscillatoriaceae. Spirulina not only has high nutritional value, but also contains a large number of physiologically active substances beneficial to the human body, and is widely used in the fields of food, medicine and bioengineering. Spirulina is rich in biologically active substances such as protein, chlorophyll, polysaccharides, β-carotene, γ-linolenic acid, and fat. The main component of spirulina is protein, which accounts for 60% -70% of the dry weight, and contains 8 kinds of amino acids and a variety of biologically active substances needed by the human body, and has special medical and health care functions...

Claims

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Application Information

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IPC IPC(8): C07K14/795C07K1/14C07D487/22C08B37/00
Inventor 陈云飞李志良
Owner 江苏海睿生物科技有限公司
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