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Method for industrially improving storage stability of transglutaminase

A technology of transglutaminase and transglutaminase enzyme, which is applied in the field of improving the storage stability of transglutaminase in industry, can solve the problems of enzyme activity and thermal stability decline, enzyme activity decline, etc. Achieve the effect of being conducive to industrial application, short time consumption, and improving storage stability

Active Publication Date: 2018-10-09
TAIXING YIMING BIOLOGICAL PRODS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The invention provides a method for improving the storage stability of transglutaminase, which solves the problem of enzyme activity and thermal stability caused by temperature changes in the production method of transglutaminase in the prior art. decline, and the problem of enzyme activity decline due to other environmental factors

Method used

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  • Method for industrially improving storage stability of transglutaminase
  • Method for industrially improving storage stability of transglutaminase

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Take 200mL of the transglutaminase fermentation broth produced by Streptomyces Maoyuan with an enzyme activity of 30U / mL, add 1:1 (v / v) ethanol precipitation fermentation broth, let stand at room temperature for 10min, centrifuge, discard The supernatant was spray-dried (inlet air temperature 160°C, feed rate 25mL / min, compressed air flow rate 200NL / min, outlet air temperature 65°C) to obtain transglutaminase enzyme preparation powder.

[0041] Measure its enzyme activity, measure its enzyme activity after standing at 37°C for one month, and calculate the degree of decrease in enzyme activity.

Embodiment 2

[0043] Compared with Example 1, add 2.5% (w / w) amino acid to the supernatant precipitate after ethanol precipitation, mix well, let stand for 30min, and then carry out spray drying; other steps and parameters are the same as those in Example 1 unanimous.

[0044] Take 200mL of the transglutaminase fermentation broth produced by Streptomyces Maoyuan with an enzyme activity of 30U / mL, add 1:1 (v / v) ethanol precipitation fermentation broth, let stand at room temperature for 10min, centrifuge, discard Supernatant, weigh the precipitate, add 2.5% (w / w) amino acid, mix well, let stand for 30min, and then carry out spray drying (inlet air temperature 160°C, feed rate 25mL / min, compressed air flow 200NL / min , air outlet temperature 65° C.), to obtain transglutaminase enzyme preparation powder.

[0045] Measure its enzyme activity, measure its enzyme activity after standing at 37°C for one month, and calculate the degree of decrease in enzyme activity.

Embodiment 3

[0047] Compared with Example 2, different exogenous additives or combinations of exogenous additives are added to the fermentation broth, stirred until completely dissolved, left to stand for a period of time, and then subsequent steps such as ethanol precipitation are performed; and other steps and parameters are the same as Example 2 is consistent. specifically:

[0048] Take 200 mL of transglutaminase fermentation broth produced by Streptomyces Maoyuan with an enzyme activity of 30 U / mL, add one or more exogenous additives, stir until completely dissolved, and let stand for 30 minutes. Add 1:1 (v / v) ethanol precipitation fermentation broth, let stand at room temperature for 10min, centrifuge, discard the supernatant, weigh the precipitate, add 2.5% (w / w) amino acid, mix well, let stand for 30min, then Spray drying (inlet air temperature 160°C, feed rate 25mL / min, compressed air flow rate 200NL / min, outlet air temperature 65°C) to obtain transglutaminase preparation powder....

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Abstract

The invention discloses a method for industrially improving the storage stability of transglutaminase, and belongs to the technical field of enzyme preparations. The method includes adding exogenous substances such as salt, carbohydrate or derivatives of the carbohydrate, proteins and alcohol in transglutaminase fermentation broth and precipitating the fermentation broth by the aid of ethyl alcohol to obtain precipitates; adding amino acid or maltodextrin into the precipitates and uniformly mixing the precipitates and the amino acid or the maltodextrin with one another; carrying out spray drying to obtain transglutaminase preparation powder. The method has the advantages that synergistic effects of the diversified exogenous substances are realized, accordingly, the enzyme activity can be effectively improved, and the yield can be effectively increased; the method is easy to operate, and effects for obviously relieving the degrees of enzyme activity decrease due to external environmental factors in transglutaminase processing procedures and transglutaminase storage procedures can be realized by the method.

Description

technical field [0001] The invention relates to a method for industrially improving the storage stability of transglutaminase, belonging to the technical field of enzyme preparations. Background technique [0002] Transglutaminase (TGase for short, EC 2.3.2.13) is a thiolase that catalyzes the formation of a protein hinge into a network by inducing the ε-(γ-glutamic acid) lysine bond between peptides. structure, thereby improving the water solubility, foamability, emulsification and thermal stability of proteins, and the special catalytic ability makes TGase widely used in food, textile, biomedical material engineering and other fields. [0003] With the general application of biotechnology and enzyme engineering technology in the industrial field, the specific active conformation of the enzyme protein, in the process of processing and storage, due to the influence of external environmental factors, when the environment changes or the interaction of some groups When weakene...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/10C12N9/96
CPCC12N9/1044C12N9/96C12Y203/02013
Inventor 史百鸣周宇益
Owner TAIXING YIMING BIOLOGICAL PRODS
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