Method for raising seedlings of cherry trees

A cherry tree seedling hardening technology, which is applied in the field of cherry tree seedling cultivation, can solve the problems of low survival rate, high cost, labor and time-consuming, etc., and achieve the effect of increasing seedling production, low cost, and easy cultivation and management

Inactive Publication Date: 2018-10-12
句容市茂润苗木有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] At present, the breeding methods of cherry trees are mainly grafting or cuttings, both of

Method used

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  • Method for raising seedlings of cherry trees

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Experimental program
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Effect test

Embodiment 1

[0021] A method for raising seedlings of cherry trees, comprising the steps of:

[0022] 1) Acquisition of cherry tree stem section explants:

[0023] Cutting 5 cm long stems containing buds from the adult cherry trees is explants, after the explants are sterilized, rinsed with sterile water, and sucked dry, the branches are cut into stems with an axillary bud;

[0024] 2) induction culture of cherry tree stem explants:

[0025] The obtained explants were induced and cultivated at a temperature of 25±2°C, a light intensity of 2000-2300 Lux, 10-13 hours of light per day, and a humidity of 40%-50%. After 35-42 days, the axillary buds began to germinate and formed small buds ; The composition of the induction medium in the induction culture is: MS+BA2.0mg / L+NAA0.2mg / L+sucrose 35g / L, pH5.5~6.5.

[0026] 3) Proliferation and cultivation of cherry tree cluster buds:

[0027] Cut the cultivated new long axillary bud seedlings into stems with only one axillary bud under sterile con...

Embodiment 2

[0036] Comparative Experiment

[0037] 1. Material selection:

[0038] Cut out 5cm-long stems containing buds from 5-8-year-old cherry trees as explants, sterilize the explants, wash them with sterile water, and absorb the water, then cut the branches with an axillary bud 80 stem segments were randomly divided into 4 groups.

[0039] Group 1: adopt the method of Example 1.

[0040] Group 2: the same method as Example 1, the difference is: in the 2nd) step, the rapid propagation method of cherry trees of the present invention is not adopted, but WPM+BA0.5~1.0mg / L+NAA0.2mg / L+sucrose 35g / L, pH5.5~6.5;

[0041] Group 3: the same method as Example 1, the difference is: in the 3rd) step, the rapid propagation method of cherry trees of the present invention is not adopted, but MS+BA1.0mg / L+NAA0.2mg / L+CH200mg is adopted / L+sucrose 35g / L, pH5.5~6.5;

[0042] Group 4: same as embodiment 1 method, difference is: in the 4th) step, do not adopt the first rooting medium composition de...

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Abstract

The invention provides a method for raising seedlings of cherry trees and belongs to the field of tree breeding. The method comprises: 1) acquiring explants of the stem segments of a cherry tree, 2) inducing and cultivating the explants of the stems of the cherry tree, 3) proliferating and cultivating multiple shoots of the cherry tree, 4) carrying out rooting culture of the cherry trees through afirst stage: culturing the single bud cut from the multiple shoots through a first rooting medium and carrying out culture under 2500-2800 Lux and illumination for 20h every day for 7 days and a second stage: transferring the seedling to a second rooting medium and carrying out rooting culture for 25-30d until good roots grow, 5) hardening and transplanting tissue culture seedlings of the cherrytree through hardening seedlings at the normal temperature for 7-14d, and transferring the seedlings into the matrix and carrying out culture for 25-30d, and 6) transplanting the tissue culture seedlings in a culture field. Compared with the traditional cherry tree grafting or cutting propagation method, the method saves labor and time, realizes a low cost, is easy to operate, realizes the survival rate of cherry tree seedlings of 97%, and can realize large-scale standard production.

Description

technical field [0001] The invention relates to a method for raising seedlings of cherry trees, which belongs to the field of tree breeding. Background technique [0002] Cherry tree, a small deciduous tree plant, about 1.05-25 meters high, dark chestnut brown bark, white early cherry bark, smooth and shiny, with horizontal stripes; leaf surface is dark green, shiny, and slightly lighter on the back; flowers Each branch has three or five flowers, corymb-shaped or racemes, with white or light pink petals; the general flowering period of cherry blossoms is from March to May. [0003] Cherry blossoms are brightly colored and have great ornamental value. In Japan, people often drink cherry blossoms in water or wine, which has good health effects. The latest research results show that cherry blossoms also have a good effect of shrinking pores and balancing oil. It is rich in natural vitamins A, B, and E. The flavonoids contained in cherry leaves also have the effects of beautif...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 丁福冬
Owner 句容市茂润苗木有限公司
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