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Application of umbilical cord blood regeneration particles and composition thereof to skin wound healing therapy

A technology for wound healing and umbilical cord blood, applied in the field of biomedicine, to prevent the formation of scars, shorten the healing time, and shorten the wound healing time

Inactive Publication Date: 2018-10-12
孔五一
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Some conditions may require additional wound healing assistance

Method used

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  • Application of umbilical cord blood regeneration particles and composition thereof to skin wound healing therapy
  • Application of umbilical cord blood regeneration particles and composition thereof to skin wound healing therapy
  • Application of umbilical cord blood regeneration particles and composition thereof to skin wound healing therapy

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Preparation of umbilical cord blood regeneration particles

[0045] In this example, the preparation of umbilical cord blood regeneration particles is exemplified.

[0046] Blood source: The umbilical cord blood is collected into a blood bag containing anticoagulant. The anticoagulant is the original sodium citrate in the blood bag, and the umbilical cord blood is transported to Preparation lab.

[0047]Conditions: The process of obtaining umbilical cord blood regeneration particles requires a complete aseptic operation.

[0048] Specific steps are as follows:

[0049] (1) The umbilical cord blood was taken and centrifuged at 200g for 10 minutes. After centrifugation, it was divided into two parts, the upper layer solution A and the lower layer precipitate A.

[0050] (2) Take the lower layer of precipitate A, add PBS at a volume ratio of 1:2-5 for slight washing, and centrifuge at 200g for 10 minutes. After centrifugation, it is divided into two parts, the upper lay...

Embodiment 2

[0057] Regeneration particles express embryonic protein-like markers

[0058] By cultivating the regenerative particles (GFP marker) obtained in Example 1 on collagen-covered glass slides, their protein expression was detected after fixing, and the staining results showed that the particle cells expressed Oct3 / 4, Nanog, and Sox2 (see figure 2 ).

Embodiment 3

[0060] Experiment of regenerative particles repairing skin wounds of diabetic mice

[0061] The mice used in the experiment were all diabetic mice, a total of 6 mice, and a 1.5cmx1.5cm skin wound was made on the back skin of each diabetic mouse. According to body weight, they were randomly divided into 2 groups with 3 animals in each group. The regeneration particles group injected the regeneration particles (GFP marker) obtained in Example 1 into the experimental diabetic mice through the tail vein, and the normal saline group injected the same volume of normal saline into the experimental diabetic mice.

[0062] At 12 days, the skin wounds of diabetic mice in all regenerative particle groups were smaller than those in the normal saline group (see image 3 ), which indicated that mice treated with regenerative particles had faster wound healing.

[0063] After the wound was repaired, the mice in the injected regenerative particle group had newly grown hairs on the wound, wh...

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Abstract

The invention discloses application of umbilical cord blood regeneration particles and a composition thereof to skin wound healing therapy. The umbilical cord blood regeneration particles have the following characteristics that the diameter range of the umbilical cord blood regeneration particles is 1-5 [mu]m; the umbilical cord blood regeneration particles contain trace of DNA components; and theumbilical cord blood regeneration particles express Oct3 / 4, Nanog and Sox2 proteins. It is found in a research that a therapeutic agent prepared from the umbilical cord blood regeneration particles and the composition thereof can remarkably promote skin wound healing, the speed rate of wound healing is obviously increased, the hemostatic effect is good, and meanwhile forming of scars can furtherbe prevented.

Description

technical field [0001] The present invention relates to the field of biomedicine, especially the fields of histocytology, regenerative medicine, disease treatment, etc., and especially relates to the application of umbilical cord blood regeneration particles and its composition in the treatment of skin wound healing. Background technique [0002] Wound healing refers to the natural process by which the body repairs damaged tissue. Most minor injuries can be healed effectively by the body itself, but some more serious wounds may require the help of stitches or other preventive measures. The healing process is a complex one, although it is impossible to see what is going on from the outside. When a wound first occurs, the body begins to tighten the damaged blood vessel, which helps restrict blood flow so that excessive bleeding does not occur. Most small wounds, this is no problem, but large wounds still bleed because the body cannot constrict blood vessels fast enough to pr...

Claims

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Application Information

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IPC IPC(8): A61K35/51A61P17/02
CPCA61K35/51A61K9/0019
Inventor 孔五一
Owner 孔五一
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