A micro-hybrid chip based on chaotic flow
A micro-mixing and chip technology, applied in mixers, laboratory containers, chemical instruments and methods, etc., can solve problems such as integration difficulties and complex microstructures, and achieve the effect of improving integration
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[0084] Preparation method of chaotic flow micro-mixer based on horseshoe transformation:
[0085] 1) Use an engraving machine or a 3D printer to make the first chip liquid inlet 5, the second chip liquid inlet 6, the chip liquid outlet 7, the T-shaped pre-mixing channel 1, and the new U-shaped "bend" on the polymer base material Folding" channel 2-2, new reverse U-shaped "bending and folding" channel 3-2, chip outlet channel 11;
[0086] 2) The substrates with microstructures are sealed by using bonding methods such as organic solvent miscible immersion bonding method or organic solvent fumigation bonding method.
Embodiment
[0088] When a=200μm, according to Figure 2 to Figure 19 The T-shaped pre-mixing channel, micro-mixing unit, and micro-mixer structure are shown, and the microstructures are processed on the polymethyl methacrylate polymer base material using an ultra-precision engraving machine.
[0089] 1) According to the volume ratio V 三氯甲烷 :V 乙醇 = 1:10 prepare 110 ml of anhydrous ethanol and chloroform miscible solution. Wet the two substrates with microstructures in the above-mentioned miscible solution respectively, fix them under a microscope with a quartz glass fixture, and put the fixed chips into a petri dish filled with the miscible solution. Put the petri dish into the drying oven immediately, set the temperature at 40°C, and bond for 10 minutes. The cross-sections of the microchannels on the micromixer chip before and after bonding assembly are as follows: Figure 22 shown.
[0090] 2) Fluorescein Isothiocyanate (FITC) and Rhodamine B (Rhodamine B) with a concentration of 1 ...
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